畜牧与饲料科学 ›› 2020, Vol. 41 ›› Issue (1): 73-76.doi: 10.12160/j.issn.1672-5190.2020.01.013

• 兽医药物科学 • 上一篇    下一篇

超声辅助提取法对黄芩总黄酮含量的影响

阿木古楞, 裴乐, 李锋, 耿万恒, 侯勇跃, 卢岩, 李子健, 刘秀丽   

  1. 内蒙古自治区农牧业科学院,内蒙古 呼和浩特 010031
  • 收稿日期:2019-12-05 出版日期:2020-01-30 发布日期:2020-03-05
  • 通讯作者: 刘秀丽(1978—),女,副研究员,博士,主要研究方向为新兽药与生物制品研究。
  • 作者简介:阿木古楞(1988—),男,硕士,主要研究方向为兽医药理学。

Effect of Ultrasound-assisted Extraction on Total Flavonoids Content in Radix scutellariae

Amuguleng, PEI Le, LI Feng, GENG Wan-heng, HOU Yong-yue, LU Yan, LI Zi-jian, LIU Xiu-li   

  1. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China
  • Received:2019-12-05 Online:2020-01-30 Published:2020-03-05

摘要: 为从不同超声条件中,筛选出黄芩总黄酮的最佳提取工艺。用超声波辅助技术提取黄芩总黄酮,用亚硝酸钠—硝酸铝比色法测定总黄酮含量。通过单因素和正交试验,确定了黄芩总黄酮的最佳提取工艺为乙醇浓度为50%,超声处理时间为25 min,料液比1∶25,超声处理温度为70 ℃。在该条件下,总黄酮提取量为15.15 mg/g。

关键词: 黄芩, 总黄酮, 超声辅助, 正交试验

Abstract: The objective of this study was to determine the optimum ultrasound-assisted extraction condition for total flavonoids from Radix scutellariae. Extraction of total flavonoids from Radix scutellariae were carried out with ultrasonic-assisted technology, and the total flavonoids content was determined using sodium nitrite-aluminum nitrate colorimetric method. Via single factor and orthogonal experiments, the optimum extraction process of total flavonoids from Radix scutellariae was determined as followed: ethanol concentration was 50%, sonication time was 25 min, material-liquid ratio was 1∶25, and sonication temperature was 70 ℃. Under these conditions, the obtained total flavonoid extraction quantity was 15.15 mg/g.

Key words: Radix scutellariae, total flavonoids, ultrasound assistance, orthogonal test

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