生物信息学软件预测猪氨基肽酶N功能与结构

doi:10.12160/j.issn.1672-5190.2021.02.001

畜牧与饲料科学 ›› 2021, Vol. 42 ›› Issue (2): 1-6.doi: 10.12160/j.issn.1672-5190.2021.02.001

• 基础研究 • 下一篇

生物信息学软件预测猪氨基肽酶N功能与结构

贾燕¹, 曹金山¹, 魏战勇²

1.内蒙古农业大学兽医学院,内蒙古呼和浩特 010018;
2.河南农业大学动物医学院,河南郑州 450046

收稿日期:2020-11-19 出版日期:2021-03-30 发布日期:2021-04-16
通讯作者: 魏战勇（1975—）,男,教授,博士,主要从事病原生物和分子病毒学研究工作。
作者简介:贾燕（1990—）,女,博士研究生,主要研究方向为病原生物和分子病毒学。
基金资助:
国家自然科学基金项目“猪δ冠状病毒受体筛选、鉴定以及S蛋白介导的细胞入侵机制研究”（3177131339）

Prediction of Function and Structure of Porcine Aminopeptidase N by Using Bioinformatic Software

JIA Yan¹, CAO Jin-shan¹, WEI Zhan-yong²

1. College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;
2. College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450046,China

Received:2020-11-19 Online:2021-03-30 Published:2021-04-16

摘要/Abstract

摘要： [目的] 研究猪δ冠状病毒（PDCoV）入侵宿主细胞的感染机制,对其特异性受体猪氨基肽酶N基因编码蛋白功能与结构进行分析。[方法] 通过多种生物信息学软件对pAPN蛋白的理化特性、亲疏水性、信号肽、跨膜区、糖基化位点、抗原表位、功能结构域及结构特征等进行预测分析。[结果] pAPN基因总长为2 892 bp,编码963个氨基酸;其中,第35~963位氨基酸为膜外蛋白区,不含信号肽但包含2个典型的功能结构域。pAPN蛋白共有23个糖基化修饰位点和42个抗原表位。pAPN蛋白胞外域空间结构为二聚体,每个单体均可分为Ⅰ~Ⅳ 4个结构域。[结论] 该研究为了解pAPN蛋白与多种猪肠道冠状病毒相互作用的机制提供了思路。

关键词: 猪氨基肽酶N, 肠道冠状病毒, 生物信息学软件, 功能, 结构

Abstract: [Objective] To investigate the underlining molecular mechanism of porcine deltacoronavirus （PDCoV） invading the host cells, the structure and function of the protein that served as a specific receptor of PDCoV and encoded by the aminopeptidase N gene （pAPN） was bioinformatically analyzed. [Method] A variety of bioinformatic software was used to predict and assess the physical and chemical properties, hydrophilicity and hydrophobicity, signal peptide, transmembrane domain, glycosylation site,antigenic epitope, functional domain, and structural characteristics of pAPN protein. [Result] The total length of pAPN gene is 2 892 bp, which encodes 963 amino acids. Among them, the 35^th~963^th amino acids are the extramembrane protein region, which do not contain signal peptide but contain two typical functional domains. pAPN protein contains 23 glycosylation modification sites and 42 antigenic epitopes. The spatial structure of extracellular domain of pAPN protein is a dimer, and each monomer can be divided into four domains of Ⅰ~Ⅳ. [Conclusion] This study provides information for understanding the molecular mechanism of interactions between pAPN protein and various porcine enteric coronaviruses.

Key words: porcine aminopeptidase N, enteric coronaviruses, bioinformatic software, function, structure

中图分类号:

贾燕, 曹金山, 魏战勇. 生物信息学软件预测猪氨基肽酶N功能与结构[J]. 畜牧与饲料科学, 2021, 42(2): 1-6.

JIA Yan, CAO Jin-shan, WEI Zhan-yong. Prediction of Function and Structure of Porcine Aminopeptidase N by Using Bioinformatic Software[J]. Animal Husbandry and Feed Science, 2021, 42(2): 1-6.

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生物信息学软件预测猪氨基肽酶N功能与结构

Prediction of Function and Structure of Porcine Aminopeptidase N by Using Bioinformatic Software

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