9株绵羊肺炎支原体内蒙古分离株P113基因克隆及蛋白序列分析

doi:10.12160/j.issn.1672-5190.2022.06.001

畜牧与饲料科学 ›› 2022, Vol. 43 ›› Issue (6): 1-5.doi: 10.12160/j.issn.1672-5190.2022.06.001

• 基础研究 • 下一篇

9株绵羊肺炎支原体内蒙古分离株P113基因克隆及蛋白序列分析

戴伶俐¹,王娜¹,白帆¹,张帆¹,宋越¹,张月梅¹,达来宝力格¹,李晓艳²,王根云²,赵世华¹

1.内蒙古自治区农牧业科学院，内蒙古呼和浩特 010031
2.呼和浩特市动物疫病防控中心，内蒙古呼和浩特 010020

收稿日期:2022-09-05 出版日期:2022-11-30 发布日期:2022-12-19
通讯作者: 赵世华（1962—），男，研究员，主要研究方向为动物传染病防控。王根云（1962—），男，高级兽医师，主要研究方向为动物疫病防控。
作者简介:戴伶俐（1983—），女，助理研究员，博士，主要研究方向为动物传染病。
基金资助:
呼和浩特市科技计划项目(2019-农-10);内蒙古自治区科技重大专项(2021ZD0023);内蒙古自治区科技重大专项(2021ZD0024);内蒙古自治区科技计划项目(2020GG0041);内蒙古农牧业创新基金项目(2020CXJJM06);内蒙古农牧业创新基金项目(2022CXJJM06)

P113 Gene Cloning and Protein Sequence Analysis of 9 Isolates of Mycoplasma ovipneumoniae from Inner Mongolia

DAI Ling-li¹,WANG Na¹,BAI Fan¹,ZHANG Fan¹,SONG Yue¹,ZHANG Yue-mei¹,Dalaibaolige ¹,LI Xiao-yan²,WANG Gen-yun²,ZHAO Shi-hua¹

1. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences，Hohhot 010031，China
2. Hohhot Municipal Prevention and Control Center for Animal Epidemic Disease，Hohhot 010020，China

Received:2022-09-05 Online:2022-11-30 Published:2022-12-19

摘要/Abstract

摘要：

[目的]克隆绵羊肺炎支原体内蒙古分离株P113基因，比对、分析不同菌株P113蛋白序列，为研究绵羊肺炎支原体P113蛋白的生物学功能提供参考。[方法]设计绵羊肺炎支原体P113基因特异性引物，采用PCR方法扩增9株绵羊肺炎支原体内蒙古分离株的P113基因片段；对获得的9株绵羊肺炎支原体P113基因片段进行测序分析，将DNA序列翻译为氨基酸序列，对不同菌株的P113氨基酸序列进行比对。[结果]不同分离株P113基因片段扩增产物大小不同。氨基酸序列分析显示，C末端序列重复区域长度存在差异，以KKAEGA（S）QNQG为主要重复序列单元。不同菌株重复序列单元数量不同，NM01-MO株和CK-MO株的重复序列单元数量最多，为16个；LK-MO株重复序列单元数量最少，为3个；多数菌株之间重复序列单元数量差异较大。[结论]绵羊肺炎支原体内蒙古分离株P113氨基酸序列的C末端重复序列单元数量不同，这些不同数量的重复序列影响P113蛋白结构，进而可能影响其生物学功能。

关键词: 绵羊肺炎支原体, P113基因, P113蛋白, 重复序列, 生物学功能

Abstract:

[Objective] The P113 genes of Mycoplasma ovipneumoniae isolates from Inner Mongolia were cloned, and the P113 protein sequences of various isolates were bioinformatically compared and analyzed, so as to provide references for clarifying the biological function of Mycoplasma ovipneumoniae P113 protein. [Method] A set of specific primers were designed to amplify the P113 gene fragments of 9 isolates of Mycoplasma ovipneumoniae from Inner Mongolia. The obtained P113 gene fragments of various isolates were sequenced. The DNA sequences were then translated into amino acid sequences, and the differences in amino acid sequences among various isolates were bioinformatically compared. [Result] The PCR amplified products of P113 gene of various isolates varied in size. According to amino acid sequence analysis, the lengths of C-terminal repetitive elements were variable. KKAEGA (S) QNQG was the dominantly observed repetitive element. The number of repetitive elements of the isolates varied. The most repetitive elements were found in the NM01-MO isolate and CK-MO isolate, both of which were 16. The least repetitive elements were found in the LK-MO isolate, which were 3. Most isolates had large differences in the number of repetitive elements. [Conclusion] The number of C-terminal repetitive elements in the amino acid sequence of P113 protein of Mycoplasma ovipneumoniae isolates from Inner Mongolia was variable. These repetitive elements had an impact on P113 protein structure, which could then have an impact on its biological activity.

Key words: Mycoplasma ovipneumoniae, P113 gene, P113 protein, repetitive elements, biological function

中图分类号:

S858.262.4

戴伶俐, 王娜, 白帆, 张帆, 宋越, 张月梅, 达来宝力格, 李晓艳, 王根云, 赵世华. 9株绵羊肺炎支原体内蒙古分离株P113基因克隆及蛋白序列分析[J]. 畜牧与饲料科学, 2022, 43(6): 1-5.

DAI Ling-li, WANG Na, BAI Fan, ZHANG Fan, SONG Yue, ZHANG Yue-mei, Dalaibaolige , LI Xiao-yan, WANG Gen-yun, ZHAO Shi-hua. P113 Gene Cloning and Protein Sequence Analysis of 9 Isolates of Mycoplasma ovipneumoniae from Inner Mongolia[J]. Animal Husbandry and Feed Science, 2022, 43(6): 1-5.

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9株绵羊肺炎支原体内蒙古分离株P113基因克隆及蛋白序列分析

P113 Gene Cloning and Protein Sequence Analysis of 9 Isolates of Mycoplasma ovipneumoniae from Inner Mongolia

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