腹泻仔猪与健康仔猪肠道微生物多样性比较

doi:10.12160/j.issn.1672-5190.2020.04.021

畜牧与饲料科学 ›› 2020, Vol. 41 ›› Issue (4): 107-110.doi: 10.12160/j.issn.1672-5190.2020.04.021

腹泻仔猪与健康仔猪肠道微生物多样性比较

特日格勒^1,2, 白雪^1,2, 段仕^1,2, 刘娜娜^1,2, 孙燕勇^1,2, 付绍印^2,3, 刘永斌³, 邬锐军⁴, 赵培厅⁴, 张文广^1,2

1.内蒙古农业大学动物科学学院,内蒙古呼和浩特 010018;
2.动物遗传育种与繁殖内蒙古自治区重点实验室,内蒙古呼和浩特 010018;
3.内蒙古自治区农牧业科学院,内蒙古呼和浩特 010031;
4.内蒙古正大有限公司,内蒙古呼和浩特 010070

收稿日期:2020-04-29 出版日期:2020-07-30 发布日期:2020-08-19
通讯作者: 张文广（1973—）,男,教授,博士,博士生导师,主要研究方向为数量基因组学与性状遗传机理。
作者简介:特日格勒（1994—）,女,硕士研究生,主要研究方向为数量基因组学与性状遗传机理。
基金资助:
2015年度内蒙古自治区草原英才团队项目

Comparison of Intestinal Microbial Diversity Between Piglets with or without Diarrhea

Terigele^1,2, BAI Xue^1,2, DUAN Shi^1,2, LIU Na-na^1,2, SUN Yan-yong^1,2, FU Shao-yin^2,3, LIU Yong-bin³, WU Rui-jun⁴, ZHAO Pei-ting⁴, ZHANG Wen-guang^1,2

1. College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China;
2. Inner Mongolia Key Laboratory of Animal Genetics,Breeding and Reproduction,Hohhot 010018,China;
3. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;
4. Inner Mongolia Zhengda Co. ,Ltd. ,Hohhot 010070,China

Received:2020-04-29 Online:2020-07-30 Published:2020-08-19

摘要/Abstract

摘要： 目的通过研究对比哺乳期腹泻仔猪与健康仔猪粪便菌群,探讨腹泻对哺乳期仔猪肠道微生物的影响。方法通过高通量16S rDNA测序技术对腹泻组仔猪（n=6）和健康组仔猪（n=3）粪便样本进行测序,比较两组仔猪肠道微生物群落的组成和结构。结果腹泻组仔猪和健康组仔猪粪便菌群差异显著（P<0.05）,腹泻组仔猪的肠道菌群多样性显著（P<0.05）低于健康组仔猪。与健康组仔猪相比,在门水平上,腹泻组仔猪的梭杆菌门和变形菌门的相对丰度显著（P<0.05）增加,而厚壁菌门的相对丰度显著（P<0.05）下降;在属水平上,腹泻组仔猪的梭杆菌属（Fusobacterium）、普雷沃菌属（Prevotella）、埃希菌属（Escherichia）、乳杆菌属（Lactobacillus）、厌氧弧菌属（Anaerovibrio）、拟普雷沃菌属（Alloprevotella）和Fusobacteriaceae_unclassified的相对丰度显著（P<0.05）增加,而大部分厚壁菌门菌属的相对丰度显著（P<0.05）降低。结论通过分析对比腹泻仔猪与健康仔猪肠道微生物多样性,为预防和治疗哺乳期仔猪腹泻提供了理论依据。

关键词: 仔猪, 肠道微生物, 腹泻, 16S rDNA

Abstract: [Objective] To assess the influence of diarrhea on intestinal microbial diversity of nursery piglets by comparing the difference in fecal flora between piglets with or without diarrhea. [Method] The fecal samples of piglets in the diarrhea group （n=6） and healthy group （n=3） were sequenced by using 16S rDNA high-throughput sequencing technology, and the composition and structure of the intestinal microbial community of the two groups were compared. [Result] The fecal flora between the diarrhea group and the healthy group was significantly （P<0.05） different, and the intestinal flora diversity of the diarrhea group was significantly （P<0.05） lower than that of the healthy group. Compared with the healthy group, at the phylum level, the relative abundance of Fusobacteria and Proteobacteria of the diarrhea group was increased significantly （P<0.05）, while that of Firmicutes was decreased significantly （P<0.05）; at the genus level, the relative abundance of Fusobacterium, Prevotella, Escherichia, Lactobacillus, Anaerovibrio, Alloprevotella and Fusobacteriaceae_unclassified of the diarrhea group was increased significantly （P<0.05）, while that of most genus belonging to Firmicutes was decreased significantly （P<0.05）. [Conclusion] The analysis and comparison of the intestinal microbial diversity between diarrhea piglets and healthy piglets provide a theoretical basis for the prevention and treatment of diarrhea in nursery piglets.

Key words: piglets, intestinal flora, diarrhea, 16S rDNA

中图分类号:

S858.287.3

特日格勒, 白雪, 段仕, 刘娜娜, 孙燕勇, 付绍印, 刘永斌, 邬锐军, 赵培厅, 张文广. 腹泻仔猪与健康仔猪肠道微生物多样性比较[J]. 畜牧与饲料科学, 2020, 41(4): 107-110.

Terigele, BAI Xue, DUAN Shi, LIU Na-na, SUN Yan-yong, FU Shao-yin, LIU Yong-bin, WU Rui-jun, ZHAO Pei-ting, ZHANG Wen-guang. Comparison of Intestinal Microbial Diversity Between Piglets with or without Diarrhea[J]. Animal Husbandry and Feed Science, 2020, 41(4): 107-110.

参考文献 21

[1]	KUBASOVA T,DAVIDOVA-GERZOVA L,MERLOT E,et al.Housing systems influence gut microbiota composition of sows but not of their piglets[J].PLoS ONE,2017,12(1):e0170051.
[2]	KONSTANTINOV S R,AWATI A A,WILLIAMS B A,et al.Post-natal development of the porcine microbiota composition and activities[J].Environmental Microbiology,2006,8(7):1191-1199.
[3]	项云,章啸君,屠平光,等.仔猪肠道微生物宏基因组的研究分析[J].浙江畜牧兽医,2016,41(5):1-4.
[4]	LALLÈS,JEAN-PAUL,BOSI P,et al. Nutritional management of gut health in pigs around weaning[J].Proceedings of the Nutrition Society,2007,66(2):260-268.
[5]	蔡亚波. 猪的消化道疾病的类型和特点[J].农民致富之友,2015(12):288.
[6]	白马伟,李芳,张英,等.云南省蒙自市仔猪腹泻致病性细菌病原分析[J].上海畜牧兽医通讯,2016(2):25-27.
[7]	华升,梅书棋,曾德芳,等.猪场仔猪腹泻的病因及综合防治[J]. 中国畜牧兽医,2007,34(3):115-117.
[8]	KIM H B,BOREWICZ K,WHITE B A,et al.Longitudinal investigation of the age-related bacterial diversity in the feces of commercial pigs[J].Veterinary Microbiology,2011,153(1/2):124-133.
[9]	CHEN L,XU Y,CHEN X,et al.The maturing development of gut microbiota in commercial piglets during the weaning transition[J].Frontiers in Microbiology,2017(8):1688.
[10]	王一冰,张小平,黄怡,等.运用454焦磷酸测序技术对断奶前后仔猪肠道菌群的分析[J].动物营养学报,2013,25(10):2440-2446.
[11]	杨巧丽. 腹泻仔猪粪便微生物结构和功能及SLA-DQA基因与粪便菌群的相关性[D].兰州:甘肃农业大学,2017.
[12]	汪群,闫鹤.腹泻仔猪与健康仔猪粪便菌群多样性的比较[J].中国畜牧杂志,2019,55(8):106-110.
[13]	韩春生. 江西地区新生仔猪肠道菌群分析及拮抗CpA乳杆菌的筛选[D].南昌:江西农业大学,2019.
[14]	STEELMAN S M,CHOWDHARY B P,DOWD S,et al.Pyrosequencing of 16S rRNA genes in fecal samples reveals high diversity of hindgut microflora in horses and potential links to chronic laminitis[J].BMC Veterinary Research,2012(8):231.
[15]	ISAACSON R,KIM H B.The intestinal microbiome of the pig[J].Animal Health Research Reviews,2012,13(1):100-109.
[16]	MAKKI K,DEEHAN E C,WALTER J,et al.The impact of dietary fiber on gut microbiota in host health and disease[J]. Cell Host Microbe,2018,23(6):705-715.
[17]	任大勇. 益生乳酸杆菌的黏附及免疫调节作用研究[D]. 长春:吉林大学,2013.
[18]	YANG Q L,HUANG X Y,ZHAO S G,et al . Structure and function of the fecal microbiota in diarrheic neonatal piglets[J].Frontiers in Microbiology,2017(8):502.
[19]	LIU H Y,ROOS S,JONSSON H,et al . Effects of Lactobacillus johnsonii and Lactobacillus reuteri on gut barrier function and heat shock proteins in intestinal porcine epithelial cells[J].Physiological Reports,2015,3(4):e12355.
[20]	ROSELLI M,FINAMORE A,BRITTI M S,et al . The novel porcine Lactobacillus sobrius strain protects intestinal cells from enterotoxigenic Escherichia coli K88 infection and prevents membrane barrier damage[J].Journal Nutrition,2007,137(12):2709-2716.
[21]	HERMANN-BANK M L,SKOVGAARD K,STOCKMARR A,et al. Characterization of the bacterial gut microbiota of piglets suffering from new neonatal porcine diarrhea[J].BMC Veterinary Research,2015,11(1):139.

腹泻仔猪与健康仔猪肠道微生物多样性比较

Comparison of Intestinal Microbial Diversity Between Piglets with or without Diarrhea

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