畜牧与饲料科学 ›› 2020, Vol. 41 ›› Issue (5): 63-66.doi: 10.12160/j.issn.1672-5190.2020.05.012

• 动物遗传与繁育 • 上一篇    下一篇

无量山乌骨鸡脂联素基因的多态性分析

唐甜甜1, 丁相红2, 陈粉粉2   

  1. 1.西南林业大学生物多样性保护学院, 云南 昆明 650224;
    2.西南林业大学生命科学学院, 云南 昆明 650224
  • 收稿日期:2020-05-12 发布日期:2020-10-21
  • 通讯作者: 陈粉粉(1976—), 女, 高级实验师, 博士, 主要研究方向为生物技术与家畜育种。
  • 作者简介:唐甜甜(1980—), 女, 讲师, 硕士, 主要研究方向为生物多样性保护。

Polymorphic Analysis of Adiponectin Gene in Wuliangshan Black-bone Chicken

TANG Tian-tian1, DING Xiang-hong2, CHEN Fen-fen2   

  1. 1. College of Biodiversity Conservation, Southwest Forestry University, Kunming 650224, China;
    2.College of Life Science, Southwest Forestry University, Kunming 650224, China
  • Received:2020-05-12 Published:2020-10-21

摘要: 采用PCR-RFLP(聚合酶链式反应—限制性片段长度多态)方法对无量山乌骨鸡208个个体的脂联素基因(NC_006096)第1外显子上的Hsp92 Ⅱ酶切位点的c.A99G突变、第2外显子上的Mva Ⅰ酶切位点c.C565G突变进行检测。结果显示, 在c.A99G 位点有AA、AB和BB 3种基因型, c.C565G位点有CC、CD和DD 3种基因型。对2个位点群体遗传学特性分析的结果表明, c.A99G位点多肽信息含量为中度多态, 而在c.C565G 位点多肽信息含量为低度多态。经χ2适合性检验表明群体在2个位点均处于平衡状态。

关键词: 无量山乌骨鸡, 脂联素基因, PCR-RFLP

Abstract: In this study, a total of 208 individuals of Wuliangshan black-bone chicken were chosen to screen the mutations of c.A99G locus in exonⅠdigested by Hsp92Ⅱand the mutations of c.C565G locus in exonⅡdigested by MvaⅠin the adiponectin gene (accession number NC_006096) via polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). There were three genotypes (AA, AB and BB) in c.A99G locus and three genotypes (CC, CD and DD) in c.C565G locus. Analysis of the population genetic characteristics showed that the information content of the peptides at the c.A99G locus was at moderate-polymorphic level, while that of the peptides at the c.C565G locus was at low-polymorphic level. The Chi-square (χ2) fitness test demonstrated that both loci of c.A99G and c.C565G of the population were in equilibrium state.

Key words: Wuliangshan black-bone chicken, adiponectin gene, PCR-RFLP

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