河南省鸡源结肠弯曲杆菌的分离鉴定及耐药性分析

doi:10.12160/j.issn.1672-5190.2021.03.021

畜牧与饲料科学 ›› 2021, Vol. 42 ›› Issue (3): 111-114.doi: 10.12160/j.issn.1672-5190.2021.03.021

河南省鸡源结肠弯曲杆菌的分离鉴定及耐药性分析

于辉¹, 李金磊², 方忠意², 巩丹²

1.河南省动物卫生监督所,河南郑州 450008;
2.河南省兽药饲料监察所,河南郑州 450008

收稿日期:2021-03-17 出版日期:2021-05-30 发布日期:2021-06-21
通讯作者: 巩丹（1983—）,女,兽医师,主要从事畜产品质量安全及微生物检测工作。
作者简介:于辉（1977—）,女,高级兽医师,硕士,主要从事动物监督与检疫工作。

Isolation,Identification and Antibiotic Resistance Analysis of Campylobacter coli from Chickens in Henan Province

YU Hui¹, LI Jin-lei², FANG Zhong-yi², GONG Dan²

1. Henan Institution of Animal Health Supervision,Zhengzhou 450008,China;
2. Henan Institute of Veterinary Drug and Feed Control,Zhengzhou 450008,China

Received:2021-03-17 Online:2021-05-30 Published:2021-06-21

摘要/Abstract

摘要： [目的]掌握河南省鸡源结肠弯曲杆菌的分布和对抗菌药物的耐药情况。[方法]从养殖场抽取鸡泄殖腔拭子460份进行结肠弯曲杆菌分离培养,采用PCR、基质辅助激光解吸电离飞行时间质谱进行鉴定,并通过微量肉汤稀释法对分离菌株进行药物敏感性分析。[结果]共分离结肠弯曲杆菌42株,分离率为9.13%;分离的结肠弯曲杆菌对环丙沙星、萘啶酸、阿奇霉素耐药严重,耐药率分别为95.24%、90.48%、71.43%。[结论]河南省鸡源结肠弯曲杆菌耐药情况较为严重,应规范使用抗菌药物。

关键词: 鸡源结肠弯曲杆菌, 分离鉴定, 耐药性分析

Abstract: [Objective] To characterize the distribution and antibiotic resistance pattern of Campylobacter coli from chickens in Henan Province. [Method] A total of 460 cloaca swab samples were collected from chicken farms to isolate the Campylobacter coli strains. The confirmative identification of the isolates was performed using PCR assay and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS）. The antimicrobial susceptibility test was carried out with broth micro-dilution method. [Result] A total of 42 Campylobacter coli strains were obtained with an isolating rate of 9.13%. High levels of resistance to ciprofloxacin, nalidixic acid and azithromycin were observed, and the resistant rate was 95.24%, 90.48% and 71.43%, respectively. [Conclusion] The antimicrobial resistant Campylobacter coli strains isolated from chickens are prevalent in Henan Province. Further attention should be given to the prudential use of veterinary antibiotics.

Key words: Campylobacter coli from chickens, isolation and identification, antibiotic resistance analysis

中图分类号:

S858.315.12

于辉, 李金磊, 方忠意, 巩丹. 河南省鸡源结肠弯曲杆菌的分离鉴定及耐药性分析[J]. 畜牧与饲料科学, 2021, 42(3): 111-114.

YU Hui, LI Jin-lei, FANG Zhong-yi, GONG Dan. Isolation,Identification and Antibiotic Resistance Analysis of Campylobacter coli from Chickens in Henan Province[J]. Animal Husbandry and Feed Science, 2021, 42(3): 111-114.

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河南省鸡源结肠弯曲杆菌的分离鉴定及耐药性分析

Isolation,Identification and Antibiotic Resistance Analysis of Campylobacter coli from Chickens in Henan Province

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