畜牧与饲料科学 ›› 2023, Vol. 44 ›› Issue (3): 96-102.doi: 10.12160/j.issn.1672-5190.2023.03.014

• 草业科学 • 上一篇    下一篇

硒引发时间对紫花苜蓿幼苗不同部位抗氧化性能的影响

阴禹舟,王勃,夏方山,王聪聪,陈奕霖,赵萍   

  1. 山西农业大学草业学院,山西 太谷 030801
  • 收稿日期:2023-03-08 出版日期:2023-05-30 发布日期:2023-07-12
  • 通讯作者: 夏方山(1983—),男,副教授,博士,主要研究方向为草类植物育种与种子科学。
  • 作者简介:阴禹舟(1997—),男,硕士研究生,主要研究方向为草类植物育种与种子科学。
  • 基金资助:
    内蒙古大学“省部共建草原家畜生殖调控与繁育”国家重点实验室开放课题(2021KF0303);山西农业大学“十四五”生物育种工程项目(YZGC134);山西省高等学校科技创新项目(2021L106);山西省基础研究计划青年科学基金项目(20210302124607);晋中市科技重点研发计划项目(Y202012)

Effects of Selenium Priming Duration on Antioxidant Capacity of Different Parts of Alfalfa Seedlings

YIN Yuzhou,WANG Bo,XIA Fangshan,WANG Congcong,CHEN Yilin,ZHAO Ping   

  1. College of Grassland Science,Shanxi Agricultural University,Taigu 030801,China
  • Received:2023-03-08 Online:2023-05-30 Published:2023-07-12

摘要:

[目的]探讨紫花苜蓿不同部位抗氧化性能对外源硒引发时间的响应,为采用种子引发技术开展富硒苜蓿草产品生产提供参考。[方法]用浓度为0.5 mmol/L的亚硒酸钠溶液对紫花苜蓿种子引发0、3、6、9、12 h后,分析不同引发时间处理下幼苗整株、子叶及胚根的超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、抗坏血酸过氧化物酶(ascorbate peroxidase,APX)、谷胱甘肽还原酶(glutathione reductase,GR)活性以及丙二醛(malondialdehyde,MDA)含量。[结果]紫花苜蓿幼苗整株、子叶及胚根的SOD、CAT、APX、GR活性均随引发时间的增加呈升高趋势,MDA含量均随引发时间的增加呈先升高后降低的趋势。整株、子叶及胚根的SOD、CAT活性均在引发0 h时显著(P<0.05)低于其他引发时间,在引发12 h时显著(P<0.05)高于其他引发时间。子叶的APX活性在引发0、3 h时显著(P<0.05)低于其他引发时间,在引发12 h时显著(P<0.05)高于其他引发时间;胚根和整株的APX活性在引发0 h时显著(P<0.05)低于其他引发时间,在引发12 h时显著(P<0.05)高于其他引发时间。子叶、胚根和整株的GR活性均在引发0 h时显著(P<0.05)低于其他引发时间,在引发12 h时最高,其中,整株GR活性在引发12 h时显著(P<0.05)高于其他引发时间。子叶的MDA含量在引发12 h时最低,显著(P<0.05)低于引发0、3 h处理;整株的MDA含量在引发3 h时升至最高,在引发6 h时下降,在引发12 h时降至最低,显著(P<0.05)低于引发0、3、6 h处理;胚根的MDA含量在引发3 h时升至最高,在引发6 h时下降,在引发12 h时降至最低,显著(P<0.05)低于其他引发时间。在相同引发时间处理下,紫花苜蓿幼苗胚根的SOD、CAT、APX、GR活性以及MDA含量均显著(P<0.05)高于子叶和整株,各指标的大小顺序均为胚根>整株>子叶。[结论]硒引发对紫花苜蓿幼苗抗氧化能力的影响与其部位和引发时间有关。紫花苜蓿幼苗整株、子叶及胚根的抗氧化酶活性随着硒引发时间的增加而上升,而MDA含量的变化趋势相反。硒引发对紫花苜蓿幼苗子叶的影响最小,对幼苗胚根的影响最大。硒引发浓度为0.5 mmol/L、引发时间为12 h时效果最佳。

关键词: 硒, 种子引发, 引发时间, 紫花苜蓿, 抗氧化酶

Abstract:

[Objective] The aim of the present study was to explore the responses of antioxidant capacity of different parts of alfalfa to the exogenous selenium priming duration, and to provide references for the production of selenium-rich alfalfa grass products by seed priming technology. [Method] Alfalfa seeds were subjected to priming treatment with 0.5 mmol/L sodium selenite solution for 0, 3, 6, 9, and 12 h, respectively. The activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) as well as the content of malondialdehyde (MDA) in the whole plant, cotyledon, and radicle of the alfalfa seedlings treated for different priming durations were determined and statistically compared. [Result] With the prolongation of selenium priming duration, the activities of SOD, CAT, APX, and GR of the whole plant, cotyledon, and radicle of the alfalfa seedlings all increased, whereas their content of MDA increased first and then decreased. For the whole plant, cotyledon, and radicle, significantly (P<0.05) lower and significantly (P<0.05) higher activities of both SOD and CAT were observed at 0 h and 12 h priming compared with the other priming durations, respectively. The APX activity of cotyledon was significantly (P<0.05) reduced at both 0 h and 3 h priming, and was significantly (P<0.05) elevated at 12 h priming. The significantly (P<0.05) lowest and the significantly (P<0.05) highest APX activity of both radicle and whole plant were observed at 0 h and 12 h priming, respectively. The whole plant, cotyledon, and radicle had the significantly (P<0.05) lowest GR activity at 0 h priming. Their GR activity all reached to the peak at 12 h priming, with a statistically significant (P<0.05) difference observed in the whole plant. The lowest MDA content of cotyledon was observed at 12 h priming, which exhibited statistically significant (P<0.05) differences compared with that at 0 h and 3 h priming. The MDA content of the whole plant peaked at 3 h priming, declined at 6 h priming, and decreased to the lowest level at 12 h priming, showing statistically significant (P<0.05) differences compared with that at 0 h, 3 h, and 6 h priming. The MDA content of the radicle peaked at 3 h priming, declined at 6 h priming, and decreased to the lowest level at 12 h priming, showing statistically significant (P<0.05) differences compared with the other priming durations. Under the same priming duration, the activities of SOD, CAT, APX, GR and the content of MDA in the radicle were all significantly (P<0.05) higher than those in the cotyledon and whole plant, with radicle being the highest followed by the whole plant and cotyledon. [Conclusion] The effects of selenium priming on the antioxidant capacity depended on the parts of the alfalfa seedlings and priming duration. The antioxidant enzyme activities of the whole plant, cotyledon and radicle of the alfalfa seedlings increased with the prolongation of selenium priming duration, while the change trend of MDA content was the opposite. Selenium priming had limited affects on the cotyledon and had great affects on the radicle. For the alfalfa seedlings, selenium priming concentration at 0.5 mmol/L (sodium selenite solution) and priming for 12 h were the optimal conditions.

Key words: selenium, seed priming, priming duration, alfalfa, antioxidant enzyme

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