巴音布鲁克草原放牧焉耆马隐孢子虫和十二指肠贾第虫感染情况调查

doi:10.12160/j.issn.1672-5190.2025.06.014

畜牧与饲料科学 ›› 2025, Vol. 46 ›› Issue (6): 101-106.doi: 10.12160/j.issn.1672-5190.2025.06.014

巴音布鲁克草原放牧焉耆马隐孢子虫和十二指肠贾第虫感染情况调查

赵辰昊¹, 赫永强¹, 王圆梦¹, 范瑞瑞¹, 徐春艳², 齐萌¹

1.塔里木大学动物科学与技术学院,新疆阿拉尔 843300;
2.塔里木大学医学院,新疆阿拉尔 843300

收稿日期:2025-10-14 出版日期:2025-11-30 发布日期:2026-01-26
通讯作者: 齐萌（1985—）,男,教授,博士,博士生导师,主要研究方向为人兽共患病病原生物学。徐春艳（1988—）,女,副教授,硕士,主要研究方向为流行病学与统计学。
作者简介:赵辰昊（1996—）,男,硕士研究生,主要研究方向为动物群发病防控。
基金资助:
国家自然科学基金项目（32260910）

Investigation of Cryptosporidium spp. and Giardia duodenalis Infections in Grazing Yanqi Horses on the Bayinbuluke Grassland

ZHAO Chenhao¹, HE Yongqiang¹, WANG Yuanmeng¹, FAN Ruirui¹, XU Chunyan², QI Meng¹

1. College of Animal Science and Technology, Tarim University, Alaer 843300, China;
2. College of Medicine, Tarim University, Alaer 843300, China

Received:2025-10-14 Online:2025-11-30 Published:2026-01-26

摘要/Abstract

摘要： [目的] 了解巴音布鲁克草原放牧焉耆马群中隐孢子虫和十二指肠贾第虫的感染情况。[方法] 随机采集放牧焉耆马无腹泻粪便样本103份,采用巢氏PCR技术结合基因测序方法,对样本中隐孢子虫和十二指肠贾第虫进行准确鉴定和分型,并构建系统发育树解析病原遗传进化关系。[结果] 基于隐孢子虫SSU rRNA基因片段的PCR检测结果显示,焉耆马群中隐孢子虫的阳性率为2.91%（3/103）,经鉴定分别为人隐孢子虫（n=2）和牛隐孢子虫（n=1）;进一步对gp60基因片段的序列分析显示,所检测到的人隐孢子虫均为IkA16G1基因亚型（n=2）。针对十二指肠贾第虫SSU rRNA基因片段进行PCR检测,发现焉耆马群中十二指肠贾第虫阳性率为1.94%（2/103）,且经鉴定均为集聚体B（n=2）。系统发育分析显示,研究获取的隐孢子虫及其亚型序列与多种宿主来源的已知序列处于同一进化支,未表现出明显遗传隔离特征;而十二指肠贾第虫集聚体B的序列与马源和人源序列共同构成一个单系群,提示该虫株具有潜在的人兽共患风险。[结论] 在巴音布鲁克草原放牧焉耆马群中,隐孢子虫和十二指肠贾第虫感染率处于较低水平,今后应推进系统性和持续性的监测工作。

关键词: 焉耆马, 隐孢子虫, 十二指肠贾第虫, 巴音布鲁克草原

Abstract: [Objective] To investigate the infection status of Cryptosporidium spp. and Giardia duodenalis in grazing Yanqi horse populations in the Bayinbuluke Grassland. [Methods] A total of 103 non-diarrheic fecal samples were randomly collected from grazing Yanqi horses. Nested PCR combined with gene sequencing was used for accurate identification and genotyping of Cryptosporidium spp. and G.duodenalis, and phylogenetic trees were constructed to analyze the genetic evolutionary relationships of the pathogens. [Results] Based on PCR detection of the Cryptosporidium spp. SSU rRNA gene fragment, the positive rate of Cryptosporidium spp. in Yanqi horses was 2.91% （3/103）, which were identified as Cryptosporidium hominis （n=2） and Cryptosporidium bovis （n=1）. Further sequence analysis of the gp60 gene fragment showed that the detected C. hominis isolates were all of the IkA16G1 subtype （n=2）. PCR detection targeting the G. duodenalis SSU rRNA gene fragment revealed a positive rate of 1.94% （2/103） in Yanqi horse population, and all were identified as Assemblage B （n=2）. Phylogenetic analysis showed that the Cryptosporidium spp. and subtype sequences obtained in this study clustered with known sequences from multiple hosts, without obvious genetic isolation characteristics; meanwhile, the G. duodenalis Assemblage B sequences formed a monophyletic group with equine- and human-derived sequences, suggesting potential zoonotic risk. [Conclusion] The infection rates of Cryptosporidium spp. and G. duodenalis in grazing Yanqi horses in the Bayinbuluke Grassland were at relatively low levels, and systematic and continuous monitoring should be promoted in the future.

Key words: Yanqi horse, Cryptosporidium spp., Giardia duodenalis, Bayinbuluke grassland

中图分类号:

S858.212.72

赵辰昊, 赫永强, 王圆梦, 范瑞瑞, 徐春艳, 齐萌. 巴音布鲁克草原放牧焉耆马隐孢子虫和十二指肠贾第虫感染情况调查[J]. 畜牧与饲料科学, 2025, 46(6): 101-106.

ZHAO Chenhao¹, HE Yongqiang¹, WANG Yuanmeng¹, FAN Ruirui¹, XU Chunyan², QI Meng¹. Investigation of Cryptosporidium spp. and Giardia duodenalis Infections in Grazing Yanqi Horses on the Bayinbuluke Grassland[J]. Animal Husbandry and Feed Science, 2025, 46(6): 101-106.

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巴音布鲁克草原放牧焉耆马隐孢子虫和十二指肠贾第虫感染情况调查

Investigation of Cryptosporidium spp. and Giardia duodenalis Infections in Grazing Yanqi Horses on the Bayinbuluke Grassland

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