基于反溶剂法的玉米醇溶蛋白-白藜芦醇纳米体系的制备、表征与抗氧化性能研究

doi:10.12160/j.issn.1672-5190.2025.06.001

摘要/Abstract

摘要： [目的] 研究基于反溶剂法的玉米醇溶蛋白-白藜芦醇纳米体系的制备、表征与抗氧化性能。[方法] 采用反溶剂法制备玉米醇溶蛋白-白藜芦醇纳米颗粒（Res-ZeinNPs）,通过正交设计优化配方,对Res-ZeinNPs进行系统表征,动态检测其在不同pH条件下的释放特性和抗氧化性。[结果] Zein质量、乙醇体积、Res质量和超纯水体积4个影响因素均对包封率有极显著（P<0.01）影响。制备Res-ZeinNPs的最优条件为：Zein质量75 mg、70%乙醇体积75 mL、Res质量2.5 mg、超纯水体积200 mL。所得颗粒包封率为98.13%,载药量为20.9%。透射电镜观察结果显示,Res-ZeinNPs颗粒呈圆球形,粒径众数150 nm左右,带负电荷。体外释放试验表明,Res-ZeinNPs具有pH响应特性,在pH 1.2和pH 7.4时,24 h累积释放率分别为38.80%和45.24%,均极显著（P<0.01）低于Res原粉。DPPH自由基清除试验表明,Res-ZeinNPs对DPPH的清除率显著（P<0.05）高于Res原粉。[结论] Res-ZeinNPs兼具较高包封率、良好缓释性能及较强的抗氧化活性,可提升白藜芦醇的稳定性与生物利用度。

关键词: 白藜芦醇, 玉米醇溶蛋白, 纳米颗粒, 反溶剂法, 正交设计, 包封率

Abstract: [Objective] To study the preparation, characterization, and antioxidant activity of resveratrol-zein nanoparticle （Res-ZeinNPs） systems based on the anti-solvent method. [Methods] Res-ZeinNPs were prepared using the anti-solvent method, and the formulation was optimized via orthogonal design. The Res-ZeinNPs were systematically characterized, and their release profiles under different pH conditions and antioxidant activity were dynamically evaluated. [Results] Four factors-zein mass, ethanol volume, Res mass, and ultrapure water volume-showed highly significant effects on encapsulation efficiency （P<0.01）. The optimal conditions for preparing Res-ZeinNPs were: zein mass 75 mg, 70% ethanol 75 mL, Res mass 2.5 mg, and ultrapure water 200 mL. The resulting particles achieved an encapsulation efficiency of 98.13% and a drug loading capacity of 20.9%. Transmission electron microscopy observations revealed that Res-ZeinNPs were spherical, with a modal particle size of approximately 150 nm and a negative charge. In vitro release studies indicated that Res-ZeinNPs exhibited pH-responsive release characteristics; at pH 1.2 and 7.4, the 24 h cumulative release rates were 38.80% and 45.24%, respectively, both highly significantly lower （P<0.01） than raw Res powder. DPPH radical scavenging assays demonstrated that Res-ZeinNPs had a significantly higher （P<0.05） DPPH scavenging rate than raw Res powder. [Conclusion] Res-ZeinNPs exhibit high encapsulation efficiency, favorable sustained-release properties, and enhanced antioxidant activity, which can improve the stability and bioavailability of resveratrol.

Key words: resveratrol, zein, nanoparticles, anti-solvent method, orthogonal design, encapsulation efficiency

中图分类号:

闫婧怡, 黄进, 高梓博, 蒋浩举, 朱恒甲. 基于反溶剂法的玉米醇溶蛋白-白藜芦醇纳米体系的制备、表征与抗氧化性能研究[J]. 畜牧与饲料科学, 2025, 46(6): 1-8.

YAN Jingyi, HUANG Jin, GAO Zibo, JIANG Haoju, ZHU Hengjia. Study on Preparation, Characterization, and Antioxidant Activity of Resveratrol-Zein Nanoparticle Systems Based on the Anti-Solvent Method[J]. Animal Husbandry and Feed Science, 2025, 46(6): 1-8.

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