畜牧与饲料科学 ›› 2025, Vol. 46 ›› Issue (6): 53-63.doi: 10.12160/j.issn.1672-5190.2025.06.008

• 草业科学 • 上一篇    下一篇

人工老化条件下不同燕麦品种种子活力鉴定与耐贮藏性评价

海霞1, 于嘉俊1, 姜超1, 杨栋2, 唐志鹏3, 肖燕子1, 李俊伟4   

  1. 1.呼伦贝尔学院农学院,内蒙古 海拉尔 021008;
    2.内蒙古自治区农牧业技术推广中心,内蒙古 呼和浩特 010010;
    3.鄂温克旗阳波畜牧业发展服务有限公司,内蒙古 鄂温克旗 021115;
    4.白城市农业科学院,吉林 白城 137099
  • 收稿日期:2025-08-12 出版日期:2025-11-30 发布日期:2026-01-26
  • 通讯作者: 李俊伟(1992—),男,博士,主要研究方向为麦类作物抗逆性。
  • 作者简介:海霞(1993—),女,讲师,博士,主要研究方向为作物抗逆性及杂交育种。
  • 基金资助:
    呼伦贝尔学院博士基金项目(2023BSJJ06);内蒙古自治区科技计划项目(2022YFDZ0088);呼伦贝尔市科技计划项目(NC2023019)

Identification of Seed Vigor and Evaluation of Storage Tolerance among Different Oat Varieties under Artificial Aging Conditions

HAI Xia1, YU Jiajun1, JIANG Chao1, YANG Dong2, TANG Zhipeng3, XIAO Yanzi1, LI Junwei4   

  1. 1. College of Agriculture, Hulunbuir University, Hailar 021008, China;
    2. Inner Mongolia Autonomous Region Agricultural and Animal Husbandry Technology Promotion Center, Hohhot 010010, China;
    3. Ewenki Banner Yangbo Animal Husbandry Development Service Co., Ltd., Ewenki Banner 021115, China;
    4. Baicheng Academy of Agricultural Sciences, Baicheng 137099, China
  • Received:2025-08-12 Online:2025-11-30 Published:2026-01-26

摘要: [目的] 探究人工老化条件下不同燕麦品种的种子活力差异及其耐贮藏性。[方法] 选取30份燕麦品种作为供试材料,采用人工老化技术模拟高温高湿贮藏条件(温度恒定45 ℃、相对湿度95%)进行老化处理(老化4 d),以未老化处理为对照(CK),测定发芽势、发芽率、发芽指数等种子活力指标,以及根冠比、根长、苗长等幼苗生长指标,通过单项测定指标的耐老化系数、综合耐老化系数、主成分分析、隶属函数和系统聚类分析法对参试的燕麦种子耐贮藏性进行综合评价。[结果] 与对照相比,人工老化处理后,各供试燕麦品种种子的发芽势、发芽率、发芽指数均显著(P<0.05)降低,其中29个品种发芽势、28个品种发芽指数、22个品种发芽率降幅超60%;平均发芽时间显著(P<0.05)延长,28个品种延长1~3 d;根冠比、苗长及根长总体呈下降趋势(仅少数品种例外)。为综合评价其耐贮藏性,通过主成分分析将7个萌发指标降维为4个相互独立的综合指标(累计贡献率达96.99%),用隶属函数计算耐贮藏性综合评价值(D值),并利用聚类分析法将供试燕麦种子的贮藏性划分为3个类型:强耐贮藏型3个(定燕2号、林纳、蒙燕1号),其D值≥0.657;中耐贮藏型15个(白燕7号、坝燕6号、福星、福瑞至、张燕4号、白燕19号、俄罗斯大白、草莜1号、青引1号、青引2号、福燕60、牧王、白燕12号、白燕17号、艾斯克),0.657>D≥0.401;弱耐贮藏型12个(加燕2号、楷模、甜燕70、贝勒Ⅱ、牧乐斯、青海444、福燕1号、甜燕1号、青燕1号、福燕2号、白燕11号、甜燕3号),0.401>D≥0.156。[结论] 筛选出的定燕2号、林纳、蒙燕1号为强耐贮藏优异种质资源;建立的“主成分分析+隶属函数+聚类分析”综合评价体系,为燕麦耐贮藏性精准评价提供了标准化方法,对指导燕麦种子高效贮藏及产业高质量发展具有重要应用价值。

关键词: 燕麦, 人工老化, 种子活力, 耐贮藏性

Abstract: [Objective] To investigate the differences in seed vigor and storage tolerance among different oat varieties under artificial aging conditions. [Methods] Thirty oat varieties were selected as experimental materials. Artificial aging technology was used to simulate high-temperature and high-humidity storage conditions (constant temperature of 45 ℃ and relative humidity of 95%) for an aging treatment of 4 days, with non-aged seeds serving as the control (CK). Seed vigor indicators, such as germination potential, germination percentage, and germination index, as well as seedling growth indicators such as root-to-shoot ratio, root length, and seedling length, were measured. The storage tolerance of the tested oat seeds was comprehensively evaluated using aging tolerance coefficients of individual indicators, comprehensive aging tolerance coefficients, principal component analysis, membership function analysis, and systematic clustering analysis. [Results] Compared with the control, the germination potential, germination rate, and germination index of all tested oat varieties significantly decreased (P<0.05) after artificial aging treatment. Specifically, the germination potential of 29 varieties, the germination index of 28 varieties, and the germination rate of 22 varieties decreased by more than 60%. The mean germination time was significantly prolonged (P<0.05), with 28 varieties increasing by 1-3 days. The root-to-shoot ratio, seedling length, and root length generally showed a downward trend (with a few varieties as exceptions). For a comprehensive evaluation of storage tolerance, seven germination indicators were reduced to four independent comprehensive indicators by principal component analysis (cumulative contribution rate of 96.99%), the membership function was used to calculate the comprehensive evaluation value for storage tolerance (D), and systematic clustering classified the storage tolerance of the tested oat seeds into three types:3 strong storage-tolerant type (Dingyan 2, Linna, Mengyan 1) with D≥0.657, 15 moderate storage-tolerant type (Baiyan 7, Bayan 6, Fuxing, Furui Zhi, Zhangyan 4, Baiyan 19, Russian White, Caoyou 1, Qingyin 1, Qingyin 2, Fuyan 60, Muwang, Baiyan 12, Baiyan 17, Aiske) with 0.657>D≥0.401, and 12 weak storage-tolerant type (Jiayan 2, Kaimo, Tianyan 70, Beile II, Mulesi, Qinghai 444, Fuyan 1, Tianyan 1, Qingyan 1, Fuyan 2, Baiyan 11, Tianyan 3) with 0.401>D≥0.156. [Conclusion] Dingyan 2, Linna, and Mengyan 1 were identified as strong storage-tolerant elite germplasm resources. The established comprehensive evaluation system combining "principal component analysis+membership function+clustering analysis" provides a standardized method for precise evaluation of oat storage tolerance, offering significant practical value for guiding efficient oat seed storage and the high-quality development of the industry.

Key words: oat, artificial aging, seed vigor, storage tolerance

中图分类号: