1株藏猪源解没食子酸链球菌的分离鉴定、生物学特性及致病性分析

doi:10.12160/j.issn.1672-5190.2026.02.015

畜牧与饲料科学 ›› 2026, Vol. 47 ›› Issue (2): 116-128.doi: 10.12160/j.issn.1672-5190.2026.02.015

• 动物疾病防控 • 上一篇

1株藏猪源解没食子酸链球菌的分离鉴定、生物学特性及致病性分析

吉克娜薇¹, 李悦燚², 蒋晓涛³, 石川⁴, 任海云⁵, 李雪郊⁵, 刘军⁶, 冯卫东², 饶开晴¹

1.西南民族大学,四川成都 610041;
2.甘孜州畜牧业科学研究所,四川康定 626700;
3.石渠县农牧农村局,四川石渠 627350;
4.康定市姑咱镇农牧综合站,四川康定 626001;
5.泸定县农牧农村和科技局,四川泸定 626100;
6.泸定县泸桥镇人民政府,四川泸定 626100

收稿日期:2025-11-17 出版日期:2026-03-30 发布日期:2026-06-25
通讯作者: 饶开晴（1971—）,女,副教授,博士,硕士生导师,主要研究方向为动物生长调控。冯卫东（1971—）,男,正高级畜牧师,主要研究方向为畜禽养殖。
作者简介:吉克娜薇（2001—）,女,硕士研究生,主要研究方向为基础兽医学。
基金资助:
甘孜州畜牧业科学研究所与西南民族大学合作项目“藏猪高效养殖关键技术与集成应用研究”（横20240047）

Isolation, Identification, Biological Characteristics, and Pathogenicity Analysis of a Streptococcus gallolyticus Strain Isolated from Tibetan Pig

Jikenawei¹, LI Yueyi², JIANG Xiaotao³, SHI Chuan⁴, REN Haiyun⁵, LI Xuejiao⁵, LIU Jun⁶, FENG Weidong², RAO Kaiqing¹

1. Southwest Minzu University, Chengdu 610041, China;
2. Animal Husbandry Research Institute of Garze Prefecture, Kangding 626700, China;
3. Bureau of Agriculture, Animal Husbandry, and Rural Affairs of Shiqu County, Shiqu 627350, China;
4. Integrated Agriculture and Animal Husbandry Station of Guza Town, Kangding City, Kangding 626001, China;
5. Bureau of Agriculture, Animal Husbandry, Rural Affairs, and Science & Technology of Luding County, Luding 626100, China;
6. People′s Government of Luqiao Town, Luding County, Luding 626100, China

Received:2025-11-17 Online:2026-03-30 Published:2026-06-25

摘要/Abstract

摘要： [目的] 鉴定四川省甘孜州某藏猪养殖场患呼吸道疾病藏猪的细菌性病原并明确其生物学特性与致病性特点。[方法] 对病料中的病原菌进行分离培养、形态观察、16S rDNA分子鉴定及全基因组测序,并开展药敏试验、生物膜形成能力测定和小鼠致病性分析,明确其生物学特性及致病性特点。[结果] 分离菌株在5%绵羊血琼脂平板上不溶血,菌落直径约1 mm,灰白色,边缘光滑;镜下观察为革兰阳性球菌,呈链状排列;利用细菌16S rDNA基因通用引物开展PCR扩增,获得约1 500 bp目的条带;基于16S rDNA基因构建系统发育树和全基因组鉴定分析,确定其为解没食子酸链球菌（Streptococcus gallolyticus）。药敏试验显示,该分离菌株对氨苄西林、青霉素、头孢氨苄、诺氟沙星、万古霉素、亚胺培南、左氧氟沙星、氯霉素、头孢他啶及氟苯尼考均敏感,对庆大霉素、多黏菌素B、四环素、阿奇霉素表现为耐药。此外,分离菌株在体外表现出强生物膜形成能力（+++）。小鼠致病性研究表明,分离菌株对6周龄小鼠具有致病性,腹腔注射后可致小鼠出现精神沉郁、食欲减退及急性死亡;剖检可见肝脏充血、肺脏淤血等典型病变;病理组织切片可见肝脏细胞坏死、肺脏炎性细胞浸润及细胞损伤等病变。[结论] 成功分离到1株藏猪源解没食子酸链球菌,该分离菌株具有较强的生物膜形成能力,对部分抗菌药物耐药,且具有较强的致病性。研究结果可为藏猪细菌性疫病精准防控及公共卫生安全提供参考。

关键词: 解没食子酸链球菌, 藏猪, 分离鉴定, 致病性

Abstract: [Objective] To identify the bacterial pathogen of Tibetan pigs with respiratory diseases in a Tibetan pig farm in Garze Prefecture, Sichuan Province, and to clarify its biological characteristics and pathogenicity. [Methods] The pathogenic bacteria in the diseased samples were isolated and cultured, followed by morphological observation, 16S rDNA molecular identification and whole-genome sequencing. Drug susceptibility testing, biofilm formation assay and mouse pathogenicity analysis were carried out to clarify its biological characteristics and pathogenicity. [Results] The isolated strain exhibited non-hemolytic activity on 5% sheep blood agar plates, with colonies approximately 1 mm in diameter, grayish-white, and smooth-edged; microscopic examination revealed Gram-positive cocci arranged in chains; PCR amplification was carried out using the universal primers for the bacterial 16S rDNA gene, and a target band of approximately 1,500 bp was obtained. Based on the phylogenetic tree constructed by 16S rDNA gene and the whole genome identification analysis, it was identified as Streptococcus gallolyticus.Drug susceptibility testing showed that the isolated strain was susceptible to ampicillin, penicillin, cephalexin, norfloxacin, vancomycin, imipenem, levofloxacin, chloramphenicol, ceftazidime and florfenicol, and resistant to gentamicin, polymyxin B, tetracycline and azithromycin. In addition, the isolated strain showed strong biofilm formation ability in vitro (+++) . The pathogenicity study in mice showed that the isolated strain was pathogenic to 6-week-old mice, and could cause depression, loss of appetite and acute death in mice after intraperitoneal injection. Necropsy revealed typical lesions such as liver congestion and lung congestion. Pathological tissue sections showed liver cell necrosis, lung inflammatory cell infiltration and cell damage. [Conclusion] A strain of S. gallolyticus from Tibetan pig was successfully isolated. The isolated strain has strong biofilm formation ability, resistance to certain antibiotics, and strong pathogenicity. The study results can provide a reference for the precise prevention and control of bacterial diseases in Tibetan pigs, as well as for public health safety.

Key words: Streptococcus gallolyticus, Tibetan pig, isolation and identification, pathogenicity

中图分类号:

S852.611

吉克娜薇, 李悦燚, 蒋晓涛, 石川, 任海云, 李雪郊, 刘军, 冯卫东, 饶开晴. 1株藏猪源解没食子酸链球菌的分离鉴定、生物学特性及致病性分析[J]. 畜牧与饲料科学, 2026, 47(2): 116-128.

Jikenawei, LI Yueyi, JIANG Xiaotao, SHI Chuan, REN Haiyun, LI Xuejiao, LIU Jun, FENG Weidong, RAO Kaiqing. Isolation, Identification, Biological Characteristics, and Pathogenicity Analysis of a Streptococcus gallolyticus Strain Isolated from Tibetan Pig[J]. Animal Husbandry and Feed Science, 2026, 47(2): 116-128.

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1株藏猪源解没食子酸链球菌的分离鉴定、生物学特性及致病性分析

Isolation, Identification, Biological Characteristics, and Pathogenicity Analysis of a Streptococcus gallolyticus Strain Isolated from Tibetan Pig

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