额敏县放牧羊寄生蜱体内沙门菌的分离鉴定、耐药性与毒力分析

doi:10.12160/j.issn.1672-5190.2026.02.014

畜牧与饲料科学 ›› 2026, Vol. 47 ›› Issue (2): 107-115.doi: 10.12160/j.issn.1672-5190.2026.02.014

额敏县放牧羊寄生蜱体内沙门菌的分离鉴定、耐药性与毒力分析

张旭¹, 赫永强¹, 郭慧琳¹, 朱婷婷^1,2, 唐于皓¹, 李静¹, 邓园萍¹, 齐萌¹

1.塔里木大学动物科学与技术学院,新疆阿拉尔 843300;
2.新疆生产建设兵团第九师农业科学研究所,新疆额敏 834601

收稿日期:2025-12-11 出版日期:2026-03-30 发布日期:2026-06-25
通讯作者: 齐萌（1985—）,男,教授,博士,博士生导师,主要研究方向为人兽共患病病原生物学。
作者简介:张旭（2001—）,男,硕士研究生,主要研究方向为动物群发病防控。
基金资助:
新疆生产建设兵团第九师科技计划项目（2023JS002）

Isolation, Identification, Drug Resistance and Virulence Analysis of Salmonella from Ticks Parasitizing Grazing Sheep in Emin County

ZHANG Xu¹, HE Yongqiang¹, GUO Huilin¹, ZHU Tingting^1,2, TANG Yuhao¹, LI Jing¹, DENG Yuanping¹, QI Meng¹

1. College of Animal Science and Technology, Tarim University, Aral 843300, China;
2. Agricultural Science Institute of the Ninth Division of the Xinjiang Production and Construction Corps, Emin 834601, China

Received:2025-12-11 Online:2026-03-30 Published:2026-06-25

摘要/Abstract

摘要： [目的] 了解新疆维吾尔自治区额敏县放牧羊蜱源沙门菌的耐药性和致病性。[方法] 从额敏县放牧羊体表采集10只活蜱,采用形态学方法鉴定蜱种类,通过细菌学培养分离蜱体内的致病菌,利用形态学鉴定和分子生物学方法对分离菌株进行鉴定,采用K-B纸片扩散法对分离菌株进行药敏试验,利用PCR扩增法检测其耐药基因和毒力基因的携带情况。[结果] 通过蜱形态学观察,10只蜱均为雌性亚洲璃眼蜱（Hyalomma asiaticum）;经形态学观察、生化试验和分子生物学鉴定,从10只蜱中分离到3株沙门菌,分别命名为分离株1、分离株2和分离株3,分离率为30.0%（3/10）。药敏试验结果显示,分离株1对链霉素和美罗培南耐药,分离株2对美罗培南、厄他培南和甲氧苄啶-磺胺甲噁唑耐药,分离株3对阿米卡星和美罗培南耐药;3株分离菌株均携带qnrS、qnrA、sul1、sul2和aadA1耐药基因;共检出10种毒力基因,均携带rck、spvB、sopE毒力基因。[结论] 额敏县放牧羊亚洲璃眼蜱源沙门菌具有一定的耐药性和致病风险,研究结果为蜱传病原菌的研究提供了数据支撑。

关键词: 沙门菌, 分离, 鉴定, 耐药性, 毒力基因, 蜱

Abstract: [Objective] To investigate the drug resistance and pathogenicity of Salmonella from ticks in grazing sheep in Emin County of Xinjiang Uygur Autonomous Region. [Methods] Ten live ticks were collected from the body surface of grazing sheep in Emin County. Morphological methods were used to identify the tick species. Bacteriological culture was employed to isolate the pathogenic bacteria in ticks. Morphological identification and molecular biology methods were applied to identify the isolated strains. The Kirby-Bauer disk diffusion method was used to perform drug susceptibility testing on the isolated strains. PCR amplification was employed to detect the carriage of drug resistance genes and virulence genes. [Results] Through morphological observation of ticks, 10 ticks were all female Hyalomma asiaticum. Three strains of Salmonella were isolated from 10 ticks based on morphological observation, biochemical tests and molecular biological identification, and were designated as isolate 1, isolate 2 and isolate 3, respectively, with an isolation rate of 30.0% (3/10). The results of drug susceptibility testing showed that isolate 1 was resistant to streptomycin and meropenem, isolate 2 was resistant to meropenem, ertapenem and trimethoprim-sulfamethoxazole, and isolate 3 was resistant to amikacin and meropenem. All three isolates carried qnrS, qnrA, sul1, sul2 and aadA1 resistance genes. A total of 10 virulence genes were detected, and all isolates carried rck, spvB, sopE virulence genes. [Conclusion] Salmonella from H. asiaticum in grazing sheep in Emin County have certain drug resistance and pathogenic risks. The research results provide data support for the study of tick-borne pathogens.

Key words: Salmonella, isolation, identification, drug resistance, virulence genes, ticks

中图分类号:

S852.61

张旭, 赫永强, 郭慧琳, 朱婷婷, 唐于皓, 李静, 邓园萍, 齐萌. 额敏县放牧羊寄生蜱体内沙门菌的分离鉴定、耐药性与毒力分析[J]. 畜牧与饲料科学, 2026, 47(2): 107-115.

ZHANG Xu, HE Yongqiang, GUO Huilin, ZHU Tingting, TANG Yuhao, LI Jing, DENG Yuanping, QI Meng. Isolation, Identification, Drug Resistance and Virulence Analysis of Salmonella from Ticks Parasitizing Grazing Sheep in Emin County[J]. Animal Husbandry and Feed Science, 2026, 47(2): 107-115.

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额敏县放牧羊寄生蜱体内沙门菌的分离鉴定、耐药性与毒力分析

Isolation, Identification, Drug Resistance and Virulence Analysis of Salmonella from Ticks Parasitizing Grazing Sheep in Emin County

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