新疆维吾尔自治区阿克苏地区牛羊体表蜱携带斑点热群立克次体调查

doi:10.12160/j.issn.1672-5190.2024.01.016

畜牧与饲料科学 ›› 2024, Vol. 45 ›› Issue (1): 107-113.doi: 10.12160/j.issn.1672-5190.2024.01.016

新疆维吾尔自治区阿克苏地区牛羊体表蜱携带斑点热群立克次体调查

马爱军¹, 李佳², 金敏¹, 金依璇¹, 刘诗语¹, 刘凯强², 刘燕¹, 甘露¹, 巴音查汗·盖力克¹

1.新疆农业大学动物医学学院,新疆乌鲁木齐 830052;
2.新疆维吾尔自治区阿克苏地区动物疫病控制诊断中心,新疆阿克苏 843000

收稿日期:2023-10-08 出版日期:2024-01-30 发布日期:2024-03-14
通讯作者: 李佳（1989—）,男,高级兽医师,硕士,主要从事动物疫病监测与流行病学调查工作。
作者简介:马爱军（1999—）,男,硕士研究生,主要研究方向为寄生虫分子生物学。
基金资助:
中日蜱传原虫病防控技术合作研究项目（2021E01001）; 新疆维吾尔自治区自然科学基金项目（2022D01F89）

Prevalence of Tick-borne Spotted Fever Group Rickettsia on the Body Surface of Cattle and Sheep in Aksu Prefecture,Xinjiang Uygur Autonomous Region

MA Aijun¹, LI Jia², JIN Min¹, JIN Yixuan¹, LIU Shiyu¹, LIU Kaiqiang², LIU Yan¹, GAN Lu¹, Bayin Chahan Gailike¹

1. College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052,China;
2. Animal Disease Control and Diagnostic Centre of Aksu Prefecture,Xinjiang Uygur Autonomous Region,Aksu 843000,China

Received:2023-10-08 Online:2024-01-30 Published:2024-03-14

摘要/Abstract

摘要： [目的]对新疆维吾尔自治区阿克苏地区牛羊体表蜱携带斑点热群（spotted fever group Rickettsia,SFGR）立克次体情况进行调查。[方法]从阿克苏地区阿克苏市（n=344）、温宿县（n=93）、沙雅县（n=98）、乌什县（n=39）随机采集牛羊体表蜱574只,以立克次体外膜蛋白B基因（ompB）和细胞表面抗原1基因（sca1）为靶基因,运用PCR方法检测立克次体携带情况,确定所属的SFGR基因型。选择2个基因的强阳性样本进行测序,将获得的序列与GenBank数据库中已登录的相关基因序列进行同源性比对,并构建系统发育树。比较4个县（市）蜱的立克次体感染率差异。[结果]对牛羊体表蜱携带的立克次体进行检测,发现存在3种SFGR基因型,分别为暂定巴布瑞立克次体（Candidatus Rickettisa barbariae）、西伯利亚立克次体（Rickettisa sibirica）、马赛立克次体（Rickettisa massiliae）。蜱样本中立克次体的总体阳性率为15.16%（87/574）,温宿县的立克次体阳性率最高,为23.66%（22/93）。在检出的3种SFGR基因型中,暂定巴布瑞立克次体为优势种（Dr=44.83%,39/87）。4个县（市）蜱的立克次体感染率存在显著差异（P=0.001,χ²=15.834）。[结论]阿克苏地区是蜱及立克次体流行区,建议加强蜱及蜱传疾病的防控。

关键词: 蜱, 立克次体, 斑点热群, 基因型, 序列分析

Abstract: [Objective] This study was conducted to investigate the prevalence of tick-borne spotted fever group Rickettsia （SFGR） on the body surface of cattle and sheep in Aksu Prefecture, Xinjiang Uygur Autonomous Region. [Method] A total of 574 ticks on the body surface of cattle and sheep were randomly collected from Aksu City （n=344）, Wensu County （n=93）, Xayar County （n=98） and Wushi County （n=39） in Aksu Prefecture. PCR assays targeting at Rickettsia outer membrane protein B gene （ompB） and surface cell antigen 1 gene （sca1） were utilized to detect the presence of Rickettsia and to determine the SFGR genotypes. The strong positive samples of the two genes were selected for sequencing. Phylogenetic trees were constructed by comparing the obtained sequences with the relevant gene sequences registered in the GenBank Database. The differences in Rickettsia infection rates in the ticks collected from the four regions were compared.[Result]The presence of 3 SFGR genotypes, Candidatus Rickettsia barbaria, Rickettsia sibirica and Rickettsia massiliae, were observed in the ticks on the body surface of cattle and sheep. The overall positive rate of Rickettsia in the tick samples was 15.16% （87/574）, with Wensu County having the highest positive rate of 23.66% （22/93）. Among the 3 detected SFGR genotypes, Candidatus Rickettsia barbaria was identified as the dominant species （Dr=44.83%, 39/87）. There were significant （P=0.001,χ²=15.834） differences in Rickettsia infection rate in the ticks among the four regions. [Conclusion]Aksu Prefecture is an endemic area for ticks and Rickettsia. It is advised to strengthen the prevention and control of tick and tick-borne diseases.

Key words: tick, Rickettsia, spotted fever group, genotype, sequence analysis

中图分类号:

马爱军, 李佳, 金敏, 金依璇, 刘诗语, 刘凯强, 刘燕, 甘露, 巴音查汗·盖力克. 新疆维吾尔自治区阿克苏地区牛羊体表蜱携带斑点热群立克次体调查[J]. 畜牧与饲料科学, 2024, 45(1): 107-113.

MA Aijun, LI Jia, JIN Min, JIN Yixuan, LIU Shiyu, LIU Kaiqiang, LIU Yan, GAN Lu, Bayin Chahan Gailike. Prevalence of Tick-borne Spotted Fever Group Rickettsia on the Body Surface of Cattle and Sheep in Aksu Prefecture,Xinjiang Uygur Autonomous Region[J]. Animal Husbandry and Feed Science, 2024, 45(1): 107-113.

参考文献

[1] KRATOU M, BELKAHIA H, SELMI R, et al.Diversity and phylogeny of cattle ixodid ticks and associated spotted fever group Rickettsia spp. in Tunisia[J].Pathogens,2023,12(4):552.
[2] 张西臣,李建华.动物寄生虫病学[M].北京:科学出版社,2017.
[3] 马广鹏,孙传范,赵娜,等.中国蜱传病主要流行趋势及防控科技对策[J].中国农业科技导报,2011,13(2):105-109.
[4] PAROLA P, PADDOCK C D, SOCOLOVSCHI C, et al.Update on tick-borne rickettsioses around the world:A geographic approach[J].Clinical Microbiology Reviews,2013,26(4):657-702.
[5] BRÖKER M. Following a tick bite:Double infections by tick-borne encephalitis virus and the spirochete Borrelia and other potential multiple infections[J].Zoonoses and Public Health,2012,59(3):176-180.
[6] HAWMAN D W, FELDMANN H. Recent advances in understanding Crimean-Congo hemorrhagic fever virus[J].F1000Research,2018,7:F1000FacultyRev-1715.
[7] CHEN Z, LIU Q, LIU J Q, et al.Tick-borne pathogens and associated co-infections in ticks collected from domestic animals in central China[J].Parasites and Vectors,2014,7:237.
[8] 布坎南,吉本斯,等.伯杰细菌鉴定手册[M].中国科学院微生物研究所《伯杰细菌鉴定手册》翻译组,译.8版.北京:科学出版社,1984.
[9] KIM H K.Rickettsia-host-tick interactions:Knowledge advances and gaps[J].Infection and Immunity, 2022, 90(9):e0062121.
[10] 郭利萍. 新疆边境地区蜱种、蜱传立克次体分布规律及分子特征研究[D].石河子:石河子大学,2017.
[11] WEI Q Q, GUO L P, WANG A D, et al.The first detection of Rickettsia aeschlimannii and Rickettsia massiliae in Rhipicephalus turanicus ticks, in Northwest China[J].Parasites and Vectors,2015,8:631.
[12] WANG Y Z, MU L M, ZHANG K, et al.A broad-range survey of ticks from livestock in Northern Xinjiang:Changes in tick distribution and the isolation of Borrelia burgdorferi sensu stricto[J].Parasites and Vectors,2015,8:449.
[13] 周鹏. 新疆南疆部分地区拉合尔钝缘蜱蜱种鉴定及立克次体检测[D].阿拉尔:塔里木大学,2022.
[14] ESTRADA-PEÑA A. Ticks as vectors: Taxonomy, biology and ecology[J].Revue Scientifique et Technique (International Office of Epizootics),2015,34(1):53-65.
[15] MERHEJ V, ANGELAKIS E, SOCOLOVSCHI C, et al.Genotyping, evolution and epidemiological findings of Rickettsia species[J].Infection, Genetics and Evolution,2014,25:122-137.
[16] DEMIR S, ERKUNT ALAK S, KÖSEOGLU A E, et al. Molecular investigation of Rickettsia spp. and Francisella tularensis in ticks from three provinces of Turkey[J].Experimental and Applied Acarology,2020,81(2):239-253.
[17] BALINANDI S, CHITIMIA-DOBLER L, GRANDI G, et al.Morphological and molecular identification of ixodid tick species (Acari:Ixodidae) infesting cattle in Uganda[J].Parasitology Research,2020,119(8):2411-2420.
[18] 孙响,张桂林,郑重,等.新疆地区扇头蜱中Candidatus Rickettsia barbariae的检测与序列分析[J].寄生虫与医学昆虫学报,2017,24(3):141-147.
[19] 李飞. 新疆南疆图兰扇头蜱鉴定及其携带部分病原检测[D].阿拉尔:塔里木大学,2017.
[20] 郗宇. 新疆南疆部分地区绵羊蜱种鉴定及蜱带立克次体检测[D].阿拉尔:塔里木大学,2020.

新疆维吾尔自治区阿克苏地区牛羊体表蜱携带斑点热群立克次体调查

Prevalence of Tick-borne Spotted Fever Group Rickettsia on the Body Surface of Cattle and Sheep in Aksu Prefecture,Xinjiang Uygur Autonomous Region

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