Abstract: The interactive teaching model is designed to promote the interaction between teachers and undergraduates in teaching process and is aimed to cultivate the creative thinking, innovative spirit, and creative ability of undergraduates. The reform of teaching content and innovation of teaching methods and teaching model in interactive teaching model of Mathematical Analysis were discussed with emphasis on cultivation of undergraduates＇ creative ability, innovation of thoughts and ideas in education and establishment of interactive teaching model of Mathematical Analysis in college. The application of this teaching model is helpful for the improvement of teaching quality of Mathematical Analysis and for the enhancement of undergraduates＇ innovative spirit and creative ability.