Abstract: As a important technique,gene mutation has been used to study and isolate important genes.Agrobacterium tumefaciens has the ability to transfer DNA to plant cells by genetic engineering,its modifications have extended the host range from plants to bacterium and fungi.Hence,Agrobacterium tumefaciens-mediated gene mutation is one of the mutation methods widely used in the last several years.In the article,the principle of the mediation,the research situation,vector types,existed problems and its feasibility all were introduced.