Abstract: The effects of β-glucan on the production performance,egg quality and immune indices of laying hens were studied in the experiment.Seven hundred and twenty healthy New Roman laying hens were randomly divided into 3 diet groups,including 100mg/kg β-glucan group,blank control group and antibiotic control group.The test lasted 56 days.The results showed that β-glucan had no significant effect on the production performance of laying hens（P 0.05）.β-glucan can increase the antibody titers of newcastle disease vaccine after immunizing 20 days,slow the decreasing trend,decrease the number of fecal E.coli after feeding 56 days significantly （P0.01）and significantly reduce the cholesterol content in yolk（P0.01）and improve the egg quality（P0.05）.