Animal Husbandry and Feed Science ›› 2011, Vol. 32 ›› Issue (5): 34-34.doi: 10.12160/j.issn.1672-5190.2011.05.013

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Determination of Diethylstilbeostrol in Feed by Liquid-liquid-liquid Micro-extraction (LLLME) Combined with High Performance Liquid Chromatography

ZHANG Zhao-hui,DING Fang-lin,JIANG Fang-jun (Hunan Biological and Electromechanical Polytechnic,Changsha 410127,China)   

  • Online:2011-05-20 Published:2011-05-20

Abstract: Liquid-liquid-liquid micro-extraction (LLLME) combined with high performance liquid chromatography was developed for the determination of diethylstilbeostrol in feed.The micro-extraction conditions were optimized.The calibration curve showed a good linearity in the range of 0.05 -5.00mg/L (r=0.998).The limit of detection was 0.5 μg/kg and the relative standard deviation was lower than 10%.The method was green,simple,sensitive and suitable for the determination of diethylstilbeostrol in feed.

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