Abstract: The course of Comprehensive Practice Training of Animal Microbiology was reformed.Based on the common cases in the clinic production and the scientific research projects with the cooperation between school and enterprise,the reform of teaching content was divided into the basic experiment,comprehensive improving experiment and research innovation experiment.The assessment contents included microscope operation and section techniques,bacteria separation,purification and identification techniques,virus separation and identification techniques,experimental design and innovation abilities.The assessment model was the combination of process assessment and diversified,multi-level and multi-angle assessment,so as to cultivate high-skill of animal husbandry and veterinary specialty in vocational and technical colleges.