Abstract: Wet lees fermenting protein feed is prepared through that the corn is treated through yeast fermentation, extraction alcohol, probiotics fermentation and sealing packaging. The power feed of hens was added with wet lees fermenting protein feed, and then the control effects on ammonia concentration of chicken house and egg yield of laying hens were studied. The results showed that if the power feed of hens was added with 15% of wet lees fermenting protein feed, the machinery feeding wasn＇t affect, and there was no mildew and denaturalization phenomenon, and the feed intake of hens increased. Compared with control, the egg yield of tested group was increased by 5.2% with an increase of 6.6 kg, and the economic benefit of tested group was increased by about 10% with an increase of 51.7 yuan. The ammonia concentration of chicken house decreased largely, and it was 22.8 mg/L less than control, which was decreased by 32.0%.