Abstract: In order to understand the production performance of alfalfa cultivars planted in greenhouse, the comparative test on six alfalfa cultivars was made . The results showed that the total hay yield of WL323 alfalfa cultivar was the highest , reaching 18 758.33 kg/hm2. The average regeneration speed of Algonquin alfalfa cultivar was 1.44 cm/d, which was obviously higher than other cultivars. The stem-leaf ratio of cultivar Algonquin with the stubble height of 5 cm was the highest, reaching 0.873 2. The fresh grass-hay ratio of Anti-thrips alfalfa with the stubble height of 5 cm was the highest, reaching 12.846 5. The crude protein content in Alfaking was the highest, being 21.98%.