Abstract: A QuEChERS Kit-UPLC/MS/MS method for simultaneously determination of 18 quinolone residues in livestock and poultry meat was developed in this study. The samples were oscillated and centrifuged in a 1% aqueous solution of acetic acid and acetonitrile （20：80, V/V）. The obtained supernatant was purified after transferring to purification pipe and was then concentrated to dry. The residues were dissolved in aqueous solution of methanol （methanol ：water=1：4） and were then filtered. The Cls chromatographic column （100 mm×2.1 mm, 1.7 μm） was used to isolate, and the 0.2% aqueous solution of formic acid and acetonitrile solution containing 0.2% formic acid were used as mobile phase to gradient elute. The 18 quinolone residues in livestock and poultry meat were qualitatively and quantitatively determined by UPLC/MS/MS. A good linear relationship of the 18 quinolone in the range of 0.5-80 ng/mL was observed, and the correlation coefficient （r） was all above 0.99. Recovery test was performed with three concentration levels of 5.0, 10.0, 50.0 μg/kg. The average recovery of the 18 quinolone ranged from 60.5% to 97.0%, the relative standard deviation ranged from 2.1% to 19.5%, and the detection limit of the method was 5.0 μg/kg. The developed method with good reproducibility, high sensitivity, short analysis time and strong ability of confirmation was suitable for the simultaneous determination of 18 quinolone residues in livestock and poultry meat.