Abstract: [ Objective ]To prepare the furazolidone artificial antigens. [ Methods ] Furazolidone （hapte） was conjugated with the carrier proteins of bovine serum albumin （BSA） and ovalbumin （OVA） to prepare artificial antigens by diazotization and glutaraldehyde methods. The prepared artificial antigens were tested by the SDS-PAGE electrophoresis, and the concentration of the coupling protein was determined by UV-visible spectrophotometer. [Results]The SDS-PAGE results showed that the migration distance for two groups of protein bands of FZ-BSA and FZ-OVA with BSA and OVA, which were the synthesized artificial antigens prepared by two kinds of methods, were changed, and the migration distance of couplings were less than their corresponding carrier proteins. [ Conclusion ]The furazolidone artificial antigens are successfully prepared.