Abstract: This paper analyzes the existing problems in construction of the curriculum system during the linking between middle and higher vocational education in terms of social accreditation, talents training program, course content design, curriculum structure, and curriculum standards. Combining with the deep cooperation between the major of Animal Husbandry and Veterinary Medicine in Heilongjiang Agricultural Economy Vocational College and animal husbandry industry as well as livestock breeding enterprises, focusing on the characteristics of animal husbandry industry in Heilongjiang Province, the highly qualified talents with comprehensive skills of ＂cultivation, prevention, therapy＂ are trained, the ＂school-enterprise cooperation＂ and ＂5 ＋ 1＂ talent training mode is jointly developed, and the regionally distinguished ＂443＂ curriculum system is constructed. Furthermore, the innovation and pioneering talent training mode in major of animal husbandry and veterinary medicine is explored and implemented, and the corresponding countermeasures are put forward from the aspect of clearing training objectives, unifying curriculum standards, improving the connection of teaching content, enhancing evaluation and assessment, and establishing teaching group linking middle and higher vocational education.