Animal Husbandry and Feed Science ›› 2018, Vol. 39 ›› Issue (1): 1-1.doi: 10.12160/j.issn.1672-5190.2018.01.001

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Optimization of Extraction Technology of Astragalus Polysaccharide by Orthogonal Design

LI Hai-ou1, CHEN Qi1, LIN Ying-hua1, LEI Wen-pu1, SUN Yao1, DUAN Peng-fei1, CHEN Zhi-yu1, SHENG Hua1, JIN Rong2, Saina1 ( 1 .College of Pharmacy, Inner Mongolia Medical University, Hohhot O10110, China ; 2. Basic Medical College, Inner Mongolia Medical University,Hohhot 010110,China)   

  • Online:2018-01-20 Published:2018-01-20

Abstract: [objective]To optimize the extraction technology of Astragalus polysaccharide. [Methods]The solid to liquid ratio,extraction duration, extraction times, and immersion duration were chosen as the experimental factors in Astragalus polysaccharide extraction, and there were three levels for each of the factor. The water reflux extraction technology of Astragalus polysaccharide was optimized by L_9(3~4) orthogonal test using Astragalus polysaccharide content as assessing index. [Results]The optimum extraction conditions were as followed: solid to liquid ratio was 1∶12, extraction duration was 1.0 h, extraction repeated 3 times, and immersion duration was 1.0 h. Under these conditions, the obtained Astragalus polysaccharide content accounted for 6.4% and 58.23% of the raw medicinal materials and crude polysaccharide, respectively. [Conclusion]The optimized extraction technology of Astragalus polysaccharide is simple and quick to operate and has good repeatability and stabilization.

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