SNP技术在猪肉溯源过程中的应用

doi:10.12160/j.issn.1672-5190.2020.06.016

Abstract

Abstract: This study aimed to screen single nucleotide polymorphism（SNP） markers that can be used for the quality traceability of Meishan pig and Shennong pig. In this experiment, PCR-RFLP method was used to detect the polymorphism of 14 SNPs from 13 genes in Meishan pig populations and Shennong pig populations, in order to find SNPs with rich polymorphism, and finally determine the combination of SNP sites that can be used for pork traceability verification test of Meishan pig and Shennong pig by sampling again. The results showed that MMP19 gene, GRN gene, NR4A1 gene each had one SNP and PSMB10 gene had two SNPs, and the heterozygous values were all greater than 0.30, which met the requirements of meat traceability. Bar codes compiled according to 5 SNPs of MMP19, PSMB10, NR4A1 and GRN genes can be used to detect the pork quality traceability of Meishan pig and Shennong pig.

Key words: pork, traceability, single nucleotide polymorphism

CLC Number:

WU Hua-li, TU Wei-long, CAO Jian-guo, ZHANG Ying-ying, WANG Hong-yang, HUANG Ji, TAN Yong-song. Application of SNP Technology in Pork Traceability[J]. Animal Husbandry and Feed Science, 2020, 41(6): 84-89.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal30.magtechjournal.com/xmysl/EN/10.12160/j.issn.1672-5190.2020.06.016

https://journal30.magtechjournal.com/xmysl/EN/Y2020/V41/I6/84

References

[1] 阮泓越. DNA指纹技术在猪个体识别和可追溯系统中的应用研究[D].北京:中国农业科学院,2010.
[2] 丁同,王仲根,汪强,等.基于物联网及DNA识别技术的牛肉溯源系统的研究[J].物联网技术,2017,7(1):18-20.
[3] 马慧鋆,余冰雪,李妍,等.食品溯源技术研究进展[J]. 2017,43(5):277-284.
[4] 赵杰. 基于SNP和SSR标记的牛肉产品溯源鉴定技术研究与应用[D].北京:中国农业科学院,2018.
[5] 张小波,何慧,吴潇,等.基于SNP标记的肉类溯源技术[J].肉类研究,2011,25(5):40-45.
[6] 吴潇,吕贝贝,王金斌,等.SNP标记用于猪肉产品DNA溯源[J].食品科学,2017,38(24):278-282.
[7] 张小波,吴潇,何慧,等.基于SNPs标记的猪肉DNA溯源技术的研究[J].中国农业科技导报,2011,13(3):85-91.
[8] 胡肄农,丁潜,纪红军,等.苏钟猪个体身份SNP识别的数字条形码编制[J].江苏农业学报,2014,30(4):779-783.
[9] 赵永贞. 猪MMP-19和MMP-23基因的分离、定位和功能初步分析[D].杨凌:西北农林科技大学,2007.
[10] 于雷. ESR、RBP4、PRLP和ACTN1基因多态性与猪繁殖性能的关系研究[D].武汉:华中农业大学,2013.
[11] 朱璟,潘章源,訾臣,等.11个猪种群体BPI基因第10外显子HpaⅡ遗传变异分析[J].中国畜牧杂志,2011,47(23):14-17.
[12] 张玉皓. 猪H2A.Z、DAZL基因分离鉴定及遗传效应分析[D].武汉:华中农业大学,2008.
[13] 杨金娥. 猪12号染色体上10个新基因的分离、定位及其与部分性状的关联分析[D].武汉:华中农业大学,2004.
[14] 赵金凤,张冬杰,谷溪,等.猪GPX5基因、FUT1基因和NCOA1基因的多态性分析[J].华北农学报,2008,23(4):69-71.
[15] 王文涛,杨秀芹,何鑫淼,等.五个品种猪MC4R基因的PCR-RFLP分析[J].黑龙江畜牧兽医,2008(10):29-30.
[16] 刘林清. 猪HSD17B1、NR4A1、ALAS1基因的分离、鉴定及功能初步研究[D].武汉:华中农业大学,2009.
[17] 吴潇. 猪泛素—蛋白酶体途径定位、序列及性状关联分析[D].武汉:华中农业大学,2006.
[18] 赵伟. RBP4,RARG,MYOG基因多态性及其与猪繁殖性能关系的研究[D].哈尔滨:东北农业大学,2008.
[19] 罗怀容,施鹏,张亚平.单核苷酸多态性的研究技术[J].遗传,2001,23(5):471-476.
[20] ROHRER G A,FREKING B A,NONNERMAN D.Single nucleotide polymorphisms for pig identification and parentage exclusion[J].Animal Genetics,2007,38(3):253-258.
[21] 吴华莉,涂尾龙,曹建国,等.基于RFLP-PCR的猪肉溯源技术研究[J].上海农业学报,2017,33(6):57-62.
[22] 胡志刚,谈永松,王林云.申农Ⅰ号杂交猪生长及胴体性能实验[J].国外畜牧学(猪与禽),2010,30(4):67-68.
[23] 谈永松,胡志刚,涂尾龙,等.申农1号猪不同杂交组合试验报告[J].国外畜牧学(猪与禽),2011,31(3):75-80.

Application of SNP Technology in Pork Traceability

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 11

Recommended Articles

Metrics

Comments

[1]	YANG Ding, LI Shengli, HU Xiaoxiao, Baohua , Sachula , LIU Wei, ZHANG Chunhua, SUN Haizhou. Research Status and Trend Analysis of Meat Quality Traceability Based on Bibliometrics [J]. Animal Husbandry and Feed Science, 2023, 44(2): 113-120.
[2]	WANG Yong-gang, WANG Wen-bo, WANG Yan-fei, WANG Xin-rui. Analysis of Changes in World Pork Production and Trade Pattern under the Background of Trade Disputes [J]. Animal Husbandry and Feed Science, 2020, 41(3): 60-64.
[3]	. Establishment of Quality and Safety Traceability System of Dairy Products in Hohhot Region of Inner Mongolia [J]. Animal Husbandry and Feed Science, 2017, 38(5): 56-56.
[4]	. Investigation and Analysis on Current Situation of Traceability System of Sheep from Pastoral Area in Inner Mongolia [J]. Animal Husbandry and Feed Science, 2016, 37(12): 93-93.
[5]	. Application of HACCP System in the Production of Chilled Pork Supplied for Hong Kong and Macau [J]. Animal Husbandry and Feed Science, 2014, 35(9): 80-80.
[6]	. Research Progress on Traceable Technologies in the Production of Livestock and Their Applications [J]. Animal Husbandry and Feed Science, 2014, 35(10): 73-73.
[7]	. Current Situation and Countermeasures of Pork Food Safety in Gaoping City of Shanxi Province [J]. Animal Husbandry and Feed Science, 2013, 34(4): 67-67.
[8]	. Study on the Key Marking Technique in the Traceability System of Animal Food [J]. Animal Husbandry and Feed Science, 2011, 32(11): 72-72.
[9]	. Construction of Retrospecting System of Animal Product Supply Chain [J]. Animal Husbandry and Feed Science, 2010, 31(10): 86-86.
[10]	. Study on Productivity and Carcass Quality of Yorkshire -songliao Black Pig Commercial Strains [J]. Animal Husbandry and Feed Science, 2009, 30(9): 105-105.
[11]	. Polymorphism Analysis on the Single Nucleotide in the 5＇ Regulatory Region of IGF-1 Gene in Goose [J]. Animal Husbandry and Feed Science, 2009, 30(2): 134-134.