奶牛乳房炎金黄色葡萄球菌的分离鉴定及药敏试验

doi:10.12160/j.issn.1672-5190.2022.01.021

Abstract

Abstract: [Objective] To investigate the prevalence of Staphylococcus aureus associated with mastitis and its sensitivity to the commonly used antibiotics in a dairy farm in Taizhou City. [Method] Nine milk samples were recovered from the dairy cows with mastitis. The bacterial strains were isolated with Baird-Parker agar medium, and the obtained isolates were subjected to phenotypic indentification by biochemical reactions as well as molecular comfirmation by PCR assay. Furthermore, the antimicrobial sensitivity test of the isolates were carried out. [Result] Six Staphylococcus strains were obtained and identified using the coagulase test and 16S rDNA sequencing, three of which were comfirmed as Staphylococcus aureus. According to antimicrobial sensitivity test, all three strains of Staphylococcus aureus were resistant to penicillin G, two strains were resistant to tetracycline, and two strains were intermidiate to erythromycin and oxacillin. [Conclusion] The Staphylococcus aureus isolates were mostly resistant to penicillin G and tetracycline, but susceptible to the other tested antibiotics.

Key words: dairy cow mastitis, Staphylococcus aureus, isolation and identification, antimicrobial sensitivity test

CLC Number:

JIA Zi-bo, PENG Jian-kang, ZHU Yan, CHEN Hai-feng, WANG Jing. Isolation,Identification and Antimicrobial Sensitivity Test of Staphylococcus aureus from Dairy Cow Mastitis[J]. Animal Husbandry and Feed Science, 2022, 43(1): 124-128.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal30.magtechjournal.com/xmysl/EN/10.12160/j.issn.1672-5190.2022.01.021

https://journal30.magtechjournal.com/xmysl/EN/Y2022/V43/I1/124

References

[1] 赵兴绪. 奶牛乳房炎的防治[M].北京:金盾出版社,2007.
[2] HAGNESTAM C,EMANUELSON U,BERGLUND B.Yield losses associated with clinical mastitis occurring in different weeks of lactation[J].Journal of Dairy Science,2007,90(5):2260-2270.
[3] 张莉莉. 奶牛乳房炎主要病原菌药物敏感性及分子特性研究[D].兰州:兰州大学,2018.
[4] 王晶. 奶牛临床型乳房炎病因调查和病原分离鉴定及药敏试验[D].杨凌:西北农林科技大学,2018.
[5] 王剑锋. 辽宁地区个体奶牛场乳房炎流行情况的调查与综合防治[D].沈阳:沈阳农业大学,2020.
[6] 卜三平. 新疆巴州地区规模化牛场奶牛乳房炎病因调查[D].石河子:石河子大学,2014.
[7] SINHA B,FRAUNHOLZ M.Staphylococcus aureus host cell invasion and post-invasion events[J].International Journal of Medical Microbiology,2010,300(2/3):170-175.
[8] 陈亚明. 奶牛乳房炎致病菌分离鉴定及快速诊断试剂盒的研发与应用[D].南宁:广西大学,2014.
[9] PITKÄLÄ A,HAVERI M,PYÖRÄLÄ S,et al. Bovine mastitis in Finland 2001-Prevalence,distribution of bacteria,and antimicrobial resistance[J].Journal of Dairy Science,2004,87(8):2433-2441.
[10] BRAKSTAD O G,AASBAKK K,MAELAND J A.Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene[J].Journal of Clinical Microbiology,1992,30(7):1654-1660.
[11] Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing(M100-S30)[S].Wayne,PA:Clinical and Laboratory Standards Institute,2020.
[12] 张峥臻,张克春.上海某牧场隐性乳房炎病原菌流行情况调查[C]//中国畜牧兽医学会2010年学术年会—第二届中国兽医临床大会论文集(上册).长春:中国畜牧兽医学会家畜内科学分会,2010:576-579.
[13] 周国燕,陈鲁喜,孙艳,等.四川凉山地区奶牛乳房炎主要病原菌分离鉴定及药敏情况分析[J].中国乳业,2021(8):88-95.
[14] 刘肖利,刘璐瑶,李镔罡,等.金黄色葡萄球菌性奶牛乳房炎调查及菌株耐药性和毒力分析[J].西北农业学报,2021,30(10):1452-1460.
[15] 张楠,张博,石玉祥.邯郸地区奶牛乳房炎病原菌的分离鉴定及耐药性分析[J].中国奶牛,2021(9):40-43.
[16] ANABELA B,ANA A,CARLA D,et al.New perspectives on the use of phytochemicals as an emergent strategy to control bacterial infections including biofilms[J].Molecules,2016,21(7):877-883.
[17] 李希强,杨亲,王雅文,等.内蒙古某牛场牛乳中大肠杆菌和金黄色葡萄球菌的分离鉴定及耐药性分析[J].黑龙江畜牧兽医,2019(5):89-92,181-182.
[18] 王斐,王彩莲,宋淑珍,等.牦牛隐性乳房炎主要病原菌及耐药和毒力基因分布情况[J].中国畜牧兽医,2020,47(1):229-239.

Isolation,Identification and Antimicrobial Sensitivity Test of Staphylococcus aureus from Dairy Cow Mastitis

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SU Fanfan, MAO Xiangqing, ZHAO Yao, XING Guofeng, WU Zihao, WU Jing. Isolation and Identification of Lactic Acid Bacteria from Camel Milk in Hutubi County of Xinjiang Uygur Autonomous Region and Their Inhibitory Effects on Staphylococcus aureus [J]. Animal Husbandry and Feed Science, 2024, 45(2): 1-8.
[2]	CHEN Junkai, YU Yuetong, YANG Bin, WANG Zekun, LI Jing, TUO Haixin, XU Junfei, QI Meng. Isolation,Identification and Characterization of Virulence and Antimicrobial Resistance of Staphylococcus aureus Strains from Porcine Milk in a Large-scale Farm in Aral City,Xinjiang Uygur Autonomous Region [J]. Animal Husbandry and Feed Science, 2024, 45(1): 114-119.
[3]	SHI Yujie,CHAI Beibei,ZHANG Yanxin. Isolation，Identification and Detection of NDM Resistance Genes of Proteus mirabilis and Morganella morganii from Ducks, and in vitro Antibacterial Test of Traditional Chinese Medicine [J]. Animal Husbandry and Feed Science, 2023, 44(5): 121-128.
[4]	ZHENG Wei, KANG Lianhe, LIU Pengbin, Terigele , LI Zijian, LI Xingyun, WANG Limei, ZHANG Yuanyuan, LIU Xiuli, SHI Pei, QIAO Jianmin. Isolation and Identification of Yeasts from Sour Whey in Xilin Gol League, Inner Mongolia [J]. Animal Husbandry and Feed Science, 2023, 44(3): 103-108.
[5]	XIONG Ju-ping, XIAO Ying-yu, ZHANG Cheng, HAN Qing-song, GAO Xiao-long, LI Zeng-kui, WEN Ying, TONG Li-na. Isolation，Identification and Antimicrobial Susceptibility Test of a Proteus mirabilis Strain from Chicken [J]. Animal Husbandry and Feed Science, 2022, 43(5): 106-111.
[6]	SONG Yue, WANG Na, ZHANG Fan, ZHANG Yue-mei, DAI Ling-li, BAI Fan, LI Xiao-yan, Gegenbaoleri , LIU Shuang-yi, WANG Gen-yun, ZHAO Shi-hua. Isolation，Identification，Antimicrobial Susceptibility Test and Pathogenicity of a Mannheimia haemolytica Strain from Goat [J]. Animal Husbandry and Feed Science, 2022, 43(5): 123-128.
[7]	WANG Na, DAI Ling-li, Wuyuntana, Bilige, ZHAO Shi-hua, Dalaibaolige. Isolation,Identification and Antimicrobial Sensitivity Test of Providencia rettgeri from Calf [J]. Animal Husbandry and Feed Science, 2022, 43(1): 119-123.
[8]	WANG Na, DAI Ling-li, Wuyuntana, Gangtuoya, ZHAO Shi-hua, Dalaibaolige. Isolation,Identification and Antimicrobial Sensitivity Test of Escherichia coli Associated with Calf Diarrhea in Xilin Gol League,Inner Mongolia [J]. Animal Husbandry and Feed Science, 2021, 42(6): 106-111.
[9]	ZHANG Yan-fei, WANG Li-fang. Research Advances on Extraction Technology of Plant Active Ingredients and Their Therapeutic Mechanism for Bacterial Mastitis in Dairy Cows [J]. Animal Husbandry and Feed Science, 2021, 42(6): 115-123.
[10]	YU Hui, LI Jin-lei, FANG Zhong-yi, GONG Dan. Isolation,Identification and Antibiotic Resistance Analysis of Campylobacter coli from Chickens in Henan Province [J]. Animal Husbandry and Feed Science, 2021, 42(3): 111-114.
[11]	QIAO Jian-min, LI Zi-jian, WANG Li-mei, ZHANG Yuan-yuan, LI Xing-yun, Naqin, KANG Lian-he, ZHENG Wei, GUO Ke-xin, ZHAO Chen, WANG Xiao-dong. Isolation and Identification of Lactic Acid Bacteria from Traditional Fermented Cream Products in Xilin Gol League, Inner Mongolia [J]. Animal Husbandry and Feed Science, 2020, 41(4): 55-59.
[12]	Amuguleng, LI Zi-jian, PEI Le, LI Feng, GENG Wan-heng, WANG Feng-wu, HOU Yong-yue, LIU Xiu-li. Antibacterial Effects of Seven Kinds of Mongolian Medicines Against Escherichia coli and Staphylococcus aureus in Vitro [J]. Animal Husbandry and Feed Science, 2020, 41(4): 101-106.
[13]	QIAO Jian-min,LI Zi-jian,CHENG Li-xin,Fengying,HOU Yong-yue,LI Yun-hua,YUE Lin-fang,WANG Li-mei,ZHANG Yuan-yuan,LI Xing-yun,Naqin,KANG Lian-he,LI Hui,WANG Le,WANG Xiao-dong. Isolation and Identification of Lactic Acid Bacteria in Fermented Yogurt Products in Inner Mongolia [J]. Animal Husbandry and Feed Science, 2020, 41(3): 51-55.
[14]	KONG Jie, LI Ting-yu, LI Tai-yuan, CHANG Hui-yun. Isolation and Identification of Bacillus circulans in Micro-ecological Preparations and Evaluation of Disinfection Technology [J]. Animal Husbandry and Feed Science, 2020, 41(2): 31-36.
[15]	PENG Hua, LI Shu-hong, NIE Jia-wei, GUO Ze-ting. Isolation, Identification and Antimicrobial Resistance Profile of Pathogenic Bacteria of Canine Pyoderma [J]. Animal Husbandry and Feed Science, 2019, 40(8): 104-106.