内蒙古锡林郭勒盟传统奶油制品中乳酸菌的分离鉴定

doi:10.12160/j.issn.1672-5190.2020.04.011

Abstract

Abstract: In this study, the lactic acid bacteria were isolated and identified from two fermented cream samples collected in Zhenglan Banner, Xilin Gol League, Inner Mongolia. A total of 15 strains of lactic acid bacteria were obtained from these samples by catalase test, Gram staining, and molecular identification targeting 16S rDNA. All of the isolates were classified into the following four species within one genus: Lactobacillus parabuchneri （n=1）, Lactobacillus plantarum （n=3）, Lactobacillus brevis （n=4） and Lactobacillus paracasei （n=7）. The results obtained in this study provide theoretical basis and bacterial resources for the industrial production of traditional dairy products and for the research and development of excellent fermentation agents in Inner Mongolia.

Key words: traditional fermented cream products, lactic acid bacteria, isolation and identification, Xilin Gol League

CLC Number:

QIAO Jian-min, LI Zi-jian, WANG Li-mei, ZHANG Yuan-yuan, LI Xing-yun, Naqin, KANG Lian-he, ZHENG Wei, GUO Ke-xin, ZHAO Chen, WANG Xiao-dong. Isolation and Identification of Lactic Acid Bacteria from Traditional Fermented Cream Products in Xilin Gol League, Inner Mongolia[J]. Animal Husbandry and Feed Science, 2020, 41(4): 55-59.

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References 18

[1]	林鑫,倪娜,张海婷,等.奶豆腐的营养、加工、贮藏及产业展望[J].北方农业学报,2016,44(3):131-134.
[2]	雅梅,哈斯其木格,陈永福,等.内蒙古传统乳制品产业发展现状调研报告[J].中国乳品工业,2016,44(7):28-30.
[3]	DEHGHAN M,MENTE A,RANGARAJAN S,et al.Association of dairy intake with cardiovascular disease and mortality in 21 countries from five continents (PURE):a prospective cohort study[J].The Lancet,2018,392(10161):2288-2297.
[4]	WADE A T,DAVIS C R,DYER K A,et al.A Mediterranean diet supplemented with dairy foods improves markers of cardiovascular risk: results from the Med Dairy randomized controlled trial[J].Nutritional Neuroscience,2018:1-13.
[5]	张勇,刘勇,张和平.世界益生菌产品研究和发展趋势[J].中国微生态学杂志,2009,21(2):185-193.
[6]	王水泉,包艳,董喜梅,等.植物乳杆菌的生理功能及应用[J].中国农业科技导报,2010,12(4):49-55.
[7]	张哲,徐海燕,于洁.俄罗斯卡尔梅克共和国酸奶油中乳酸菌的多样性分析[J].中国乳品工业,2015,43(2):37-40,43.
[8]	郭壮. 益生菌L. casei Zhang对发酵乳质地、风味及感官特性的影响[D].呼和浩特:内蒙古农业大学,2009. 倪春梅.乳酸菌分离筛选及其对新型半硬质干酪品质特性影响[D].呼和浩特:内蒙古农业大学,2013.
[9]	杨彦荣,任艳,德亮亮,等.传统奶酪样品中乳酸菌的分离鉴定[J].中国乳品工业,2015,43(9):19-22.
[10]	国家市场监督管理总局,国家卫生健康委员会.食品微生物学检验乳酸菌检验:GB 4789.35—2016[S].北京:中国标准出版社,2017.
[11]	周德庆,徐士菊.微生物学词典[M].天津:天津科学技术出版社,2005.
[12]	李德宇. 石油烃降解复合菌群的构建及降解动力学研究[D].济南:山东交通学院,2018.
[13]	乔健敏,郑重,岳林芳,等.湿地土壤中纤维素降解菌的分离筛选研究[J].畜牧与饲料科学,2019,40(10):9-13.
[14]	呼斯楞,刘红新,于洁,等.内蒙古呼伦贝尔地区传统发酵乳中乳酸菌的多样性分析[J].微生物学通报,2016,43(5):984-990.
[15]	王宏梅,于洁,包秋华,等.利用16S rDNA序列及tuf-RFLP鉴定蒙古国发酵乳中的乳酸菌[J].中国乳品工业,2011,39(7):4-7.
[16]	MOURAD K,NOUR-EDDINE K .Physicochemical and microbiological study of ′shmen′, a traditional butter made from camel milk in the sahara (Algeria):Isolation and identification of lactic acid bacteria and yeasts[J].Grasas Y Aceites,2006,57(2):198-204.
[17]	GAO M L,HOU H M,TENG X X,et al.Microbial diversity in raw milk and traditional fermented dairy products (Hurood cheese and Jueke) from Inner Mongolia,China[J].Genetics and Molecular Research,2017,16(1):16019451.
[18]	IDOUI T,BOUDJERDA J,LEGHOUCHI E,et al . Lactic acid bacteria from ′Sheep′s Dhan′,a traditional butter from sheep′s milk: Isolation,identification and major technological traits[J].Grasas Y Aceites,2009,60(2):177-183.

Isolation and Identification of Lactic Acid Bacteria from Traditional Fermented Cream Products in Xilin Gol League, Inner Mongolia

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