哺乳动物精子获能信号通路及检测方法研究进展

doi:10.12160/j.issn.1672-5190.2023.03.009

Abstract

Abstract:

Capacitation is a crucial stage before sperm and egg cell complete fertilization in mammals. A series of physiological and biochemical processes are altered in accompany with sperm capacitation, including ion content changes, plasma membrane remodeling, cholesterol efflux, and protein tyrosine phosphorylation. In addition, decapacitation factors enable sperms to successfully complete fertilization. These alternations can be used to detect sperm capacitation status. The commonly used methods include the detections of sAC-cAMP-PKA pathway, plasma membrane fluidity change, cholesterol redistribution, tyrosine phosphorylation, and acrosome reaction. This article reviews the research progress in signaling pathways, signaling molecules, regulatory factors, ion channels, and sperm capacitation detection methods involved in sperm capacitation in mammals, and prospected the main research directions in the future, in hoping to provide references for sperm in vitro culture and the application of assisted reproductive technology.

Key words: sperm capacitation, decapacitation factor, signaling pathway, detection method

CLC Number:

Q954.44
S814

MAO Fei, CHENG Min, CHEN Xiaoliang, DUAN Ran, SUN Yang, LI Guojun, WANG Caiyun. Research Progress in Sperm Capacitation Associated Signaling Pathways and Detection Methods in Mammals[J]. Animal Husbandry and Feed Science, 2023, 44(3): 63-68.

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References 33

[1]	CHANG M C. Fertilizing capacity of spermatozoa deposited into the fallopian tubes[J]. Nature, 1951, 168(4277):697-698.
[2]	AUSTIN C R. Observations on the penetration of the sperm in the mammalian egg[J]. Australian Journal of Scientific Research Ser B: Biological Sciences, 1951, 4(4):581-596. doi: 10.1071/bi9510581 pmid: 14895481
[3]	JIN S K, YANG W X. Factors and pathways involved in capacitation: How are they regulated?[J]. Oncotarget, 2017, 8(2):3600-3627. doi: 10.18632/oncotarget.v8i2
[4]	TAKEI G L, TOURZANI D A, PAUDEL B, et al. Activation of cAMP-dependent phosphorylation pathways is independent of ROS production during mouse sperm capacitation[J]. Molecular Reproduction and Development, 2021, 88(8):544-557. doi: 10.1002/mrd.23524 pmid: 34318548
[5]	NARESH S, ATREJA S K. The protein tyrosine phosphorylation during in vitro capacitation and cryopreservation of mammalian spermatozoa[J]. Cryobiology, 2015, 70(3):211-216. doi: 10.1016/j.cryobiol.2015.03.008
[6]	STIVAL C, PUGA M L D, PAUDEL B, et al. Sperm capacitation and acrosome reaction in mammalian sperm[J]. Advances in Anatomy, Embryology, and Cell Biology, 2016, 220:93-106.
[7]	GADELLA B M, BOERKE A. An update on post-ejaculatory remodeling of the sperm surface before mammalian fertilization[J]. Theriogenology, 2016, 85(1):113-124. doi: 10.1016/j.theriogenology.2015.07.018 pmid: 26320574
[8]	ZALAZAR L, STIVAL C, NICOLLI A R, et al. Male decapacitation factor SPINK3 blocks membrane hyperpolarization and calcium entry in mouse sperm[J]. Frontiers in Cell and Developmental Biology, 2020, 8:575126. doi: 10.3389/fcell.2020.575126
[9]	LEFIÈVRE L, JHA K N, DE LAMIRANDE E, et al. Activation of protein kinase A during human sperm capacitation and acrosome reaction[J]. Journal of Andrology, 2002, 23(5):709-716. pmid: 12185106
[10]	BREITBART H, ROTMAN T, RUBINSTEIN S, et al. Role and regulation of PI3K in sperm capacitation and the acrosome reaction[J]. Molecular and Cellular Endocrinology, 2010, 314(2):234-238. doi: 10.1016/j.mce.2009.06.009 pmid: 19560510
[11]	候振. 水牛精子获能前后蛋白质组学初步研究[D]. 南宁: 广西大学, 2019.
[12]	LAWSON C, GOUPIL S, LECLERC P. Increased activity of the human sperm tyrosine kinase SRC by the cAMP-dependent pathway in the presence of calcium[J]. Biology of Reproduction, 2008, 79(4):657-666. doi: 10.1095/biolreprod.108.070367 pmid: 18562702
[13]	MITCHELL L A, NIXON B, BAKER M A, et al. Investigation of the role of SRC in capacitation-associated tyrosine phosphorylation of human spermatozoa[J]. Molecular Human Reproduction, 2008, 14(4):235-243. doi: 10.1093/molehr/gan007 pmid: 18245108
[14]	KUMARESAN A, JOHANNISSON A, HUMBLOT P, et al. Oviductal fluid modulates the dynamics of tyrosine phosphorylation in cryopreserved boar spermatozoa during capacitation[J]. Molecular Reproduction and Development, 2012, 79(8):525-540. doi: 10.1002/mrd.22058 pmid: 22674908
[15]	GANGWAR D K, ATREJA S K. Signalling events and associated pathways related to the mammalian sperm capacitation[J]. Reproduction in Domestic Animals, 2015, 50(5):705-711. doi: 10.1111/rda.12541 pmid: 26294224
[16]	GUÉRIN P, FERRER M, FONTBONNE A, et al. In vitro capacitation of dog spermatozoa as assessed by chlortetracycline staining[J]. Theriogenology, 1999, 52(4):617-628. doi: 10.1016/S0093-691X(99)00157-0
[17]	MITCHELL L A, NIXON B, AITKEN R J. Analysis of chaperone proteins associated with human spermatozoa during capacitation[J]. Molecular Human Reproduction, 2007, 13(9):605-613. doi: 10.1093/molehr/gam043 pmid: 17595329
[18]	胡志红. 大鼠附睾特异性基因ESc-615和HongrES1的研究[D]. 上海: 中国科学院研究生院(上海生命科学研究院), 2002.
[19]	WEIGEL MUÑOZ M, BATTISTONE M A, CARVAJAL G, et al. Influence of the genetic background on the reproductive phenotype of mice lacking cysteine-rich secretory protein 1 (CRISP1)[J]. Biology of Reproduction, 2018, 99(2):373-383. doi: 10.1093/biolre/ioy048 pmid: 29481619
[20]	陈云蕾. NYD-SP27基因对绵羊精液品质及精子信号通路的影响[D]. 石河子: 石河子大学, 2019.
[21]	MIZRAHI R, BREITBART H. Mitochondrial PKA mediates sperm motility[J]. Biochimica et Biophysica Acta, 2014, 1840(12):3404-3412. doi: 10.1016/j.bbagen.2014.09.005 pmid: 25219457
[22]	WERTHEIMER E, KRAPF D, DE LA VEGA-BELTRAN J L, et al. Compartmentalization of distinct cAMP signaling pathways in mammalian sperm[J]. Journal of Biological Chemistry, 2013, 288(49):35307-35320. doi: 10.1074/jbc.M113.489476 pmid: 24129574
[23]	LEFIÈVRE L, DE LAMIRANDE E, PHD C G. The cyclic GMP-specific phosphodiesterase inhibitor, sildenafil, stimulates human sperm motility and capacitation but not acrosome reaction[J]. Journal of Andrology, 2000, 21(6):929-937. pmid: 11105920
[24]	HARRISON R A P, MILLER N G A. cAMP-dependent protein kinase control of plasma membrane lipid architecture in boar sperm[J]. Molecular Reproduction and Development, 2000, 55(2):220-228. doi: 10.1002/(SICI)1098-2795(200002)55:2<220::AID-MRD12>3.0.CO;2-I pmid: 10618662
[25]	PINI T, DE GRAAF S P, DRUART X, et al. Binder of Sperm Proteins 1 and 5 have contrasting effects on the capacitation of ram spermatozoa[J]. Biology of Reproduction, 2018, 98(6):765-775. doi: 10.1093/biolre/ioy032 pmid: 29415221
[26]	FLESCH F M, BROUWERS J F, NIEVELSTEIN P F, et al. Bicarbonate stimulated phospholipid scrambling induces cholesterol redistribution and enables cholesterol depletion in the sperm plasma membrane[J]. Journal of Cell Science, 2001, 114(Pt 19):3543-3555. doi: 10.1242/jcs.114.19.3543 pmid: 11682613
[27]	BROMFIELD E G, AITKEN R J, GIBB Z, et al. Capacitation in the presence of methyl-β-cyclodextrin results in enhanced zona pellucida-binding ability of stallion spermatozoa[J]. Reproduction, 2013, 147(2):153-166. doi: 10.1530/REP-13-0393
[28]	ASQUITH K L, BALEATO R M, MCLAUGHLIN E A, et al. Tyrosine phosphorylation activates surface chaperones facilitating sperm-zona recognition[J]. Journal of Cell Science, 2004, 117(Pt 16):3645-3657. doi: 10.1242/jcs.01214 pmid: 15252132
[29]	ARCELAY E, SALICIONI A M, WERTHEIMER E, et al. Identification of proteins undergoing tyrosine phosphorylation during mouse sperm capacitation[J]. The International Journal of Developmental Biology, 2008, 52(5/6):463-472. doi: 10.1387/ijdb.072555ea
[30]	JIMÉNEZ I, GONZÁLEZ-MÁRQUEZ H, ORTIZ R, et al. Changes in the distribution of lectin receptors during capacitation and acrosome reaction in boar spermatozoa[J]. Theriogenology, 2003, 59(5/6):1171-1180. doi: 10.1016/S0093-691X(02)01175-5
[31]	AITKEN R J, BRINDLE J P. Andrology: Analysis of the ability of three probes targeting the outer acrosomal membrane or acrosomal contents to detect the acrosome reaction in human spermatozoa[J]. Human Reproduction, 1993, 8(10):1663-1669. doi: 10.1093/oxfordjournals.humrep.a137910 pmid: 8300825
[32]	MARTIN-HIDALGO D, GIL M C, HURTADO DE LLERA A, et al. Boar sperm hyperactivated motility is induced by temperature via an intracellular calcium-dependent pathway[J]. Reproduction, Fertility, and Development, 2018, 30(11):1462-1471. doi: 10.1071/RD17549
[33]	SCHMIDT H, KAMP G. Induced hyperactivity in boar spermatozoa and its evaluation by computer-assisted sperm analysis[J]. Reproduction, 2004, 128(2):171-179. doi: 10.1530/rep.1.00153 pmid: 15280556

Research Progress in Sperm Capacitation Associated Signaling Pathways and Detection Methods in Mammals

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