乳酸乳球菌食品级双筛选标记的分泌型表达载体的构建

doi:10.12160/j.issn.1672-5190.2020.02.014

Abstract

Abstract: Cellulase is widely used in animal husbandry industry, feed industry, biotechnology and bioenergy utilization. Cellulase gene engineering, as one of the hot topics in biological fermentation researches, can improve enzyme yield and stability and has broad application prospects. The pMG36e vector was transformed by using genetic engineering technology, and a food-grade recombinant plasmid with double-selected markers and external secretion function of cellulase was successfully constructed. The plasmid was then transformed into Enterococcus faecalis 19855 strain and gene expression was determined. The secreted cellulase activity was 0.92 U/mL. Our results lay a foundation for the further development of food-grade engineering bacteria.

Key words: cellulose, vector construction, gene expression, food-grade engineering bacteria

CLC Number:

Q786

SU Shao-feng, Sachula, WANG Xiao, WU Qing-hai, Qiburi, Gaowa, ZHAO Jun-li, LIU Hong-kui, WANG Yun-hua, Huhe. Construction of Lactococcus lactis Food-grade Double-screening-labeled Secretory Expression Vector[J]. Animal Husbandry and Feed Science, 2020, 41(2): 66-77.

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Construction of Lactococcus lactis Food-grade Double-screening-labeled Secretory Expression Vector

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