Abstract: This experiment was designed to study the effect of replacement of feeding antibiotics with the combination of a fermented feed and probiotics prepared with fermented Chinese herbal medicine on production performance of laying hens. The 304-day-old Hy-line laying hens were selected and randomly divided into control group and experiment group. The control group was fed with basal diet with 2 kg/t of ampicillin, while the experiment group was fed with basal diet with the combination of 3% of fermented feed and 3 kg/t of probiotics prepared with fermented Chinese herbal medicine. The results showed that the fecal formability of the experiment group was significantly better than that of the control group, and the egg laying rate was significantly increased on the 46^th and 175^th experimental day （P〈0.05）; on the 175^th day, the death and culling rate and egg breaking rate of the experiment group were reduced by 25.00%, 18.05%, respectively, and the yolk color was significantly different from that of the control group （P〈0.01）; the antibody levels against avian influenza H9 virus and Newcastle disease virus in eggs in the experimental group were significantly higher than those in the control group （P〈0.05）. In summary, the combination uses of the fermented feed and probiotics prepared with fermented Chinese herbal medicine may serve as a good alternative for feeding antibiotics in laying hen breeding.