Evaluation unit is formed by overlaping county soil data,present landuse map,agrotype map and administrative map;the appraisal factors were ensured including 13 factors from six aspects of production performance,land management,site condition,section composition,soil physical and chemical properties and the nutrient;the degree of membership is ensured by Terfel method and analytic hierarchy process by definite weight.The comprehensive evolution on farmland soil data of Laixi City was made with MAPGIS software.The result showed that the cultivated land fertility are mainly two,three and four,respectively accounting for the proportion of 28.0%,39.0%,20.7% in total cultivated area of Laixi City.

The concrete process of preparation of microneedle used in heterogeneity nucleus transplantation was introduced combining with the experience of heterogeneity nucleus transplantation in yak in the article.Meanwhile,the existed problems and effect factors were summarized.

In the study,small intestinal mucosal structure,muscular layer thickness and the distributions and numbers of intraepithelial lymphocytes（IELs） and goblet cells（GCs） from Sunit sheep at 6 months old age was determined by Histological and Histochemical methods.The results showed the length of Duodenum villus were the longest,its length attained（353.25±60.48） μm,the Jejunum and Ileum in villus length were（317.27±72.02） μm and（275.36±47.62） μm respectively,but there was markedly different between Jejunum and Ileum（P0.05）;In mucosal thickness,Duodenum were the thickest reaching（669.15±139.04） μm,the Jejunum and Ileum were（586.72±134.50） μm and（551.85±91.32） μm,Duodenum in thickness was extremely different comapared with that of the Ileum and Jejunum（P0.01）;In the thickness of muscular layer,Ileum was the thickest reaching（280.45±58.33） μm,Jejunum was（167.16±42.63） μm and Duodenum was（148.78±38.36） μm,the difference was marked among them（P0.01）;In the depth of crypt,Duodenum was the deepest reaching（309.36±74.21） μm,Jejunum was（286.23±57.23） μm,the difference was marked among them（P0.01）;In the value of V/C,Duodenum and Jejunum were（1.80±0.45） and（2.00±0.48） respectively,there was different between Jejunum and Ileum（P0.01）;In the number of IELs and GCs,all parts of intestinal mucosal layer were not significantly different（P0.05）.The conclusion could be made that the length of villus,the mucosal thickness and the depth of crypt were gradually become smaller,while the muscular layer thickness were gradually become greater in small intestine from anterior to posterior,but there were no change of IELs and GCs numbers in all parts of small intestine.

A microwave digestion procedure was developed for trace Pb in cigarette adhesive using microwave digestion system,and the amounts of these trace metallic element was determined by atomic absorption spectrometry after microwave digestion.The recovery of Pb was on the top of 97% with the RSD of 0.5%.The results showed that this method was rapid,simple,minimized environmental contamination and the samples are completely digested,the chemical reagents were economized during the sample preparation.The precision and accuracy are satisfactory.

In the study,absorption maximum wavelength of 510 nm was used as index for the orthogonal design mode to screen the optimization parameter of rutin extraction from Sophora japonica,extracted rutin was used for super oxide anion radical method for antioxidant activity experiments.The results showed that alcohol extraction was the most ideal method,and its parameters was ethanol concentration of 80%,ethanol volume of 100 mL and ultrasound temperature of 45℃,rutin of 11.6% could be achieved by it.There was dose-dependant relation for rutin to clean up free radicle O2-·,and the scavenging index of IC50 was 0.030 5 g/L,which is significant differences exist（t=2.93,P0.05）.

Archaea is one of the most popular research,it not only could survive under extreme environment,such as high temperature,high salinity,strong acid,strong base and anoxic environments,but also exist in various ecological environments.With the huge abundance and the variety of metabolic product,archaea is playing a highly important role in the biogeochemistry processes in the world the study of archaea is important to elucidate the basic laws of life and movement,revealing the interaction between biology and environment.In the article,Methanoarchaea in rumen was introduced,and its diversity also was discussed based on the analysis of 16S rDNA,which has been applied to anaylze more extreme environments of marine archaea.Nowadays,16S rDNA technology is being expanded in the rumen archaeal research,and 16S rDNA gene library of methanogenic archaea was established to study its diversity of rumen archaea.

Different breeds of meat sheep fed under different stabling conditions,tissue biopsies was applied to detected sheep abomasum tissue changes in histology.The result showed that the abomasum of Dorset sheep was thickest,the next was the Mongolian sheep＇s,the thinnest was German Merino sheep＇s,but there were significant differences among them（P 0.05）;In wrinkles of the lower gastric mucosa,Suffolk sheep fold gastric muscle was the thickest and Mongolian sheep is the thinnest;In the wrinkle heights of abomasum Gastric area,there were markedly different among Mongolian sheep,Dorset sheep and Suffolk sheep（P0.01）,but there were no significant difference among Mongolian sheep,Dekesaier sheep and Germany Merino sheep（P 0.05）;In gastric Helicobacter and Suffolk bottom,there was significantly different between Dekesaier sheep and German Merino sheep（P 0.05）.

Waterfowl are the natural repository of avian influenza virus.To study the pathogenic molecular basis of highly pathogenic avian influenza from waterfow,8 recombinant PBD plasmids and rescued the recombinant virus were constructrd by reverse genetics techniques.The total RNA was extracted from the allantoic fluids of chick embryo with the infection of A/duck/HuBei/49/05 virus,PB2,PB1,PA,HA,NP,NA,M and NS were amplified by RT-PCR respectively,and they were cloned into PBD vector.Moreover,they also were transfected into 293 T cells.The result indicated that the recombinant virus was successfully rescued and named as R-DKHB.

Death receptor 6（DR6 or TNFRSF21）is a member of TNF receptor superfamily.DR6 is a type-Ⅰtrans-membrane receptor possessing four extra -cellular cysteinerich motifs and a cytoplasmic death domain.DR6 is expressed in most human tissues and abundant transcript can be detected in heart,brain,placenta,pancreas,thymus,lymph node and several non-lymphoid cancer cell lines.DR6 can interact with TRADD and active both JNK and NF-κB.In addition,DR6 inhibits proliferation and activation of T and B cell,and it also involved in inducing neurons cell apoptosis.

By immunohistochemistry,the CD3 ＋ T lymphocytes and CD4 ＋ and CD8 ＋ T cell subsets in chicken cecal tonsil,including appearance,migration,tissue localization and changes of the number,were determined by applying monoclonal antibodies of CD3,CD4 and CD8.The results showed that CD3 ＋,CD8 ＋ T lymphocytes first appeared on 11 day-old embryo,while CD4 ＋ T lymphocytes in the 15th day.Changes in distribution,CD3 ＋ cell smainly in the mucosal epithelium,CD4 ＋ cells in the mucosa in the lamina propria,but CD8 ＋ cells in the lamina propria and mucosal epithelium.Changes in the amounts,the number of positive cells were all increased dramatically before the 7th day,and at the 7th day,CD3 ＋ cells significantly decreased in the number,but CD4 ＋,CD8 ＋ T cells had no significant changes in the number.From the 21st day to the 35th day,CD3 ＋,CD4 ＋ and CD8 ＋ T lymphocytes in the number increase continuously.The conclusion could be made that the immune function can be enhanced in the early post-hatched chicken duodenum.

By applying the principal component analysis method,mineral elements in Qinghai Yak＇s bone was quantitated and qualitied combined with SPSS software to expand a new field for comprehensive evaluation.

In China,although shrubs are rich resources and high nutritional value,the limitation of its exploitation and usage has been eixsted.Based on the above metioned,its function of anti-nutritional effects of phenolic substances in shrubs was preliminarily discussed.

In the article,the comparative study of the bioactivity was carried out between eight yeast strains and four mold strains to screen good strain with high activity.The biomass,the crude protein content,living bacteria number,fermentative fragrance of eight yeast strains were determined,and the biomass and synthesis of the enzymes of four mold strain was measured.The result indicated that yeast of Saccharomyces cerevisiae BC,BD,Candida utilis BY,Candida tropicalis BR and Aspergillus niger H3 had the excellent production performance,which can be selected as experimental strains of probiotics for ruminants.

The trial was designed with three factors and three levels and the aim was to investigate effect of addition of the different proportion the green fodder on Landes goose growth performance.180 Landes gooses with close body weight at 1 day age were randomly divided into nine groups,there were four repeats in every group and 5 Landes goose in a repeats.The result indicated that the young goose in average day appetite（ADA） was higher than that of the control between the groups of green fodder and non-green fodder,and the difference was marked（P0.05）,but average weight again（AWA） and feed conversion rate were no differences among them（P0.05）,the effect of ADA was the best one with the ratio of 1：2 between green feed and fined feed.In the middle of growth,these groups in ADA and AWA were different,the optimization condition was green feed and fined feed proportion is（1：2） to（3：4）.In the adult,the suitable green feed should be added up to the inhered feed,and it not only can satisfy the need of development at the different stage,but also it is essential to fills and feed for the later period.

Under the condition of grazing on the sandy grassland in Xilinguole steppt,Inner Mongolia,the study was made to compare with the grazing behavior,performance and the meat quality between hybrid sheep,introduced sheep and Mongolian sheep,and the effect of age on the performance and the meat quality was also discussed.

In the study,Wuzhumuqin sheep was selected to tested,the greenhouse feeding effect of daily weight gain of Wuzhumuqin sheep was determined by control trial with the selection of different typical model of raising house,the control group was free-pasturing all the year.The results showed that gestation period of female livestock was fed by house and fined feeding,which could keep increase continuously to a certain degree in the next year,and the yearly weight gain was higher that of the control,the difference was marked（P0.05）,meanwhile the female in weight gain also was different at the different stage.The different varieties female livestock in daily weight gain was the same,the weight gain was higher that that of the control,but there was markedly different between female sheep and caprine（P0.05）.The result of T test showed that lamb in daily weight gain all was higher that of the control at the different stage.Hence,greenhouse feeding has significance to change traditional raising model.

The sweet sorghum straw can be used as high energy materials to produce fuel ethanol and its by-product is stillage.The basic composition of stillage from fermentation with different techniques was analyzed.The results showed that crude protein content in stllage was the highest with inoculation of 0.25% bacteria,activated for 2 hours,fermented for 5 days.Therefore,sweet sorghum straw stillage after processed can be used as crude feed for ruminants to improve the comprehensive utilization rate of straw.

To develop the available usage of un-normal feed source in Lin＇ an,henon bamboo leaf,abundance in the region,was reasonably exploited and processed into particle feed to meet the demands of fine quality goat.Meanwhile,the trial on compound particle feed mixed with henon bamboo leaf and was carried out feeding adult goat and lamb.The result showed that the weight gain in the trial goats was the better,and economics efficiency,social effects and ecological benefit all got improvement.

Micro-ecologicalagent was paid attention for it frees from pollution,it not only can improve the raising environment and decrease the content of ammonia and sulfureted hydrogen markedly,but also enhance animal immunity.However,the disadvantages also existed obviously,so it is necessary to use it reasonably.In the article,the application of micro -ecologicalagent was reviewed,the prospect of application was also predicted.

Based on social and economic aspects in Abaga Banner ranches,the present station on family pasture was investigated,and the animal performance of Simmental cattle and grassland production was analyzed with the comparative between the model of feeding in winter and traditional grazing.Body weight,body condition,chest length and body length of Simmental cattle were detected every 3 weeks.The result indicated that all indexes of Simmental cattle with house feeding model in winter was higher than traditional grazing,and the body weight loss was less than that of the control,about 0.217 kg in average body weight increase every day.Moreover,the trial group in production also was higher than that of the control.

Diarrhea,one of the serious illnesses affecting the growth and development of domestic animals in the scale animal and poultry cultivation,hinders the development of the raising industry.Diarrhea has been become the main causes to death and growth retardation of animal,specially to young livestock,such as piglets and chicken.Nowadays,the addition of antibiotics,hormones and the synthesized chemical drugs often used to prevent or treat it,thus it lead to drug resistance and drug residue.Therefore,fermentation products added in the feeds include probiotics,vitamins,amino acids,enzymes and so on get rapid development.In the article,the application of microbial feed additive and its production technique was reviewed.

Even at the low protein ration,urea in the rumen through cycling can provide some nitrogen for synthesis of microbal proteins.In this article,the process of transporting urea across rumen epithelium and its influencing factors were reviewed.

In the study,the contrast experiment was carried out to clarity extraction technique for leaf protein concentration（LPC）,it involved in some critical factors including on the beating time,the watering proportion and the isolation method.Meanwhile,it should explore manufacture technical process and provide a effective path for LPC.

The paper introduces some identification methods to different cultivar based on DNA molecular markers,and the suggestion also was put forward in the article.

Alfalfa is the perennial leguminous plant with rich stem leaf,comprehensive nutrition,high protein content,so it is an ideal raw material for withdrawing the leaf protein.After fresh alfalfa tender stem and leaf was smashed and squeezed,raw proteins were extracted and isolated by centrifugation,then dried to gain the aim proteins.In the article,its usage and the comprehensive utilization of surplus residual was reviewed.

Under the different nitrogen additions,biomass allocation for Stipa krylovii community ground and underground was investigated.The result showed that the nitrogen additions obviously can improve biomass allocation for Stipa krylovii community ground and underground,especially to ground biomass,meanwhile the community belowground biomass allocation was reduced,but different levels of underground biomass allocation were little affected by nitrogen gradients.

Artemisia anethifolia was employed to study the relationship of salt tolerance with Chlorophyll and Betacyanin contents at the different concentration of NaCl.The results showed that Chlorophyll in seedling of A.anethifolia took the tendency of low-high-low as the increase of salt concentration,and Betacyanin contents in beet had an obvious tendency.It was concluded that the content of Chlorophyll in A.anethifolia and Betacyanin in beet could be used as important appraisal indexes to salt tolerance of A.anethifolia.

This experiment was carried out to assess the growth characterization of tolerance-salt to Saussurea runcinata.The seedling stage of S.runcinata was respectively stressed by NaCl and Na2SO4 at the concentration of（0,60,120,180,240,300 and 360 mmol/L）,and these indexes were determined,including root-top ratio,relative growth rate,root length and plant height.The results showed that root-top ratio and root length of S.runcinata were greater than that of the control under salt stress,but the relative growth rate and plant height of S.runcinata were less than that of the control;Root-top ratio of S.runcinata is no significant difference under the same concentration of two kinds salt stress,but the relative growth rate of S.runcinata under NaCl stress is significantly higher than that of Na2SO4 stress.

In the study,the extraction from stem and leaf of alfalfa was used to investigate the Allelopathic Effect on Germination and Seedling Growth of triticale.The result indicated that the concentration of extract can restrain germination of triticale seed to a certain degree,the inhibition also enhanced as the concentration of extract increased;the weight change in root,stem and leaf of triticale was increased firstly,and then decreased as the concentration of extract increased;when the extraction concentration was 20%,the effect to growth speed and height of triticale was the most significant.

To accelerate the generation of feed production techniques and improve the nutritional level of livestock fed with grass,7 strains of corn,including Silage 67,Jingang 50,Silage 2,Xingzhu 1,Jinling 7,Jinling 10 and Jinling 14,were introduced by Grassland Working Station of Hangjinhou County of Inner Mongolia in 2009,the comparative trial was carried out.The result indicated that the fresh yield of Xingzhu 1 was the highest one,but it was the lowest in GI;the series of Jinling both were the better in yield and GI,which should be generated at river irrigation district.

The total-flavonoid in Stellera chamaejasme L.was extracted by ultrasonic technology and its content was determined by colorimetry,and the cleanup rate of the polyphenolic extract towards superoxide anion radical and hydroxyl radical also was determined with control of ascorbic acid.The result showed that the content of total-flavonoid were 24.30%,and the flavonoids extract from S.chamaejsme L.had remarkable function of removing superoxide anion radical and hydroxyl radical in vitro and it was stronger than VC.The conclusion could be made that it is a promising natural antioxidant for the exploitation and development.

It is impossible to abolish the sheep rearing manner of dispersion now,restrictions and rejection to small raisers is a long process.In the future,policy and financial support should be given to sheep raisers who have a certain scale,the protection consciousness in environment and understanding of the operation and management,and it should guide them for expanding the raising scale and standardization of culture gradually.Systematic innovation should be adopted to lead the sheep industry into a high-yield,efficient,ecological,and secure direction with a large scale and high standardization,along the trace of the sheep backyard farmers,family farm or stock farm.

The comprehensive farm of Laobagang Town is located in Hai＇an County,Nantong City,Jiangsu Province.According to its output value data between 2003 and 2009,development trend of its output value in 2010 was predicted with GM（1,1） model,linear regression model and autoregressive model.

In the study,the relation between oviposition quantity of flat-chest turtle and individual body length,body breadth,body weight was determined,and the exploration on artificial proliferation,cultivation and its reasonable utilization was emphasized.The result showed that with the increase of individual body length,body breadth and body weight,oviposition quantity of flat-chest turtle also increase starting from sexual maturity in the natural multiplication,when individual body length,body breadth and body weight was（25.20±0.26） cm,（17.70±0.33） cm and（1631±27） g respectively,the oviposition quantity attained the expected,however these indexes increase further,the oviposition quantity fall back with lower format.

With the development of crab -breeding,the disease continue to occur mainly due to environmental factors,pathogenic microorganisms,as well as farming techniques,therefore healthy breeding in crabs would be paid more attention in order to decrease the damage caused by diseases,improve survival rate and keep the increase of economic efficiency.This article on these three aspects of environmental factor,pathogenic microorganism and breeding technology provide an overview of the situation home and abroad,and summed up the crab disease causes.

In the study,8 week-old ICR mice were selected as the experimental object.The trial animals were fed with different concentration sucrose under long-term stimulation,then they were killed by nitrogen,duodena were obtained,immunohistochemistry method was applied to detect villus shape and the expression level of α-gustducin,meanwhile blood sugar concentration change of mice also was determined intragastric administration before and afterwards.The result showed that rate of duodena velvet rise with the increase of sugar concentration,but there was no significant change in expression of α-gustducin.The conclusion could be made that with stimulation prolongation,the ability of tolerance to sugar enhancement.

A nutritional beverage was developed by combination of black kerneled rice and milk.Appearance,texture,taste and flavor were chosen as sensory index and the prescription was determined by orthogonal experiment.In addition,the stability also were analyzed by turbiscan technology using six months shelf-life UHT milk as control.The results showed that the prepared sample containing 50.0% milk,5.5% black kerneled rice powder,0.20% emulsifier,4.0%sugar had the best sensory character.For the stabilities of black kerneled rice milk,one compound-stabilizer including 0.20% Microcrystalline Cellulose（MCC）,0.15% modified starch and 0.02% к-carrageenan was better than the another one composed of 0.2% MCC and 0.15% modified starch.Moreover,the stabilities of samples were both better than that of the control.Therefore,two above compound-stabilizers can satisfied the need of the stability of black kerneled rice milk in six months shelf-life.

With the development of society,the demands of application talented man in high tech are increasing.Practice teaching has been become the critical link for training the ability of practice and originating consciousness,therefore to enhance it will be helpful to improve student＇s comprehensive quality,training the ability of practice and originating consciousness and strengthen their employment abilities.Based on veterinary medicine and prologue specialty is a strongly practicable subject,enhancing it should be paid attention to experience teaching,lab administration,construction of practice base and the improvement of teaching ability in practice teaching.

The core thinking to the reform of employment guiding course lies in reconstruction of course culture.New course culture can improve student＇s mental health,society communication and ability in employment,hence employment guiding should be penetrated into the whole process of learning.We practice the model of employment guiding course：vocational career should be schemed in the first term,education of mental health should be made in the second term,the education of society intercourse in the third term,employment education in the fourth term,and vocational field and guiding of vocational individuation should be educated in the fifth and sixth term respectively.If they can be fused into the integration organically,and form the model of employment guiding course,it will make students employed.

To avoid the disadvantages of traditional examination and change the present studying station and excite the positive studying,our researcher group explored a system which is suitable for Veterinary Epidemiology education in minority region,including checking,proposition,identification of cases,simulate discuss and finishing the report on trials.

Veterinary Pharmacology is an important base course for veterinary medicine specialty,and it is also a bridge between basic veterinary medicine and clinic veterinary medicine.Based on the existed problem,including difficulty in its studying,remembering and application,cultivation in student＇s ability of innovation,professional English and scientific research thinkings should be paid attention.In the practice,with the improvement of teachers＇ qualities and reform teaching methods,the interest and passion to study the subject should be promoted,and make students ability in scientific research consciousness get improvement and meet the needs of cultivating talents for animal medicine specialty.

In the article,based on the analysis of training target of vocational professionals,the training target etalon for animal pharmaceutical production and the marketing professional was positioned scientifically,and the constructive principles of the vocational curriculum system also was put forward.With the satisfying of the above the demands,the workflow for system of higher vocational education courses was developed and curriculum system was constructed scientifically,and the existed problems also emphasized.

Many factors take effect on the hatchability rate of eggs,it＇s important to improve the hatching rate by applying the correct way of laying up eggs and suitable ventilation time.The comparative tests on the way of laying up eggs and ventilation time was made in the study.The result indicated that the fertilization to hatching eggs and hatching rate markedly improved by the project that the small head upwards,the big head downwards,and shift to an earlier time for ventilated time during the process of preservation,the difference is extremely marked（P0.01）.

The major histocompatibility complex with abundant polymorphism is not only related to the resistance in pig disease,but also the reproduce capability,growth capability and the flesh quality.Therefore,as an effective molecular genetic mark,the major histocompatibility complex will be fit for the disease resistance and seeding of local pig species,and it has the important prospect of science research and practical value of application.

As a branch of genetics,epigenetics is relative to the inheritable expression change of gene or protein,without altered gene sequence,its research objective is gene modification,including DNA methylation,histone acetylation,gene marker and so on.In the recent years,with the studying development,the application on curing tumor has been achieved success in DNA methyltransferase and histone acetyltransferases inhibitors.Here we reviewed the mechanisms of the above mentioned epigenetic modification,their roles in tumor initiation,and the progresses in tumors therapies.

In the trial,ovocyte of cattle was used as material and trichostatin-A was used as suppressor of de-acetylase.The intensity of Acetylization of histone H4K12 was determined by laser confocal microscopy technique to explore the activity of transacetylase（HAT） and histon de-acylase（HDAC） during reduction division phase of Ⅰand Ⅱ,the treatment concentration of TSA was 80 μmol/L.The result indicated that fluorescence intensity of histone H4K12 couldn＇t be detected during reduction division phase,and it could be detected from matured ovocytes under the culture condition of TSA,but histone H4K12 disappear completely after the ovocytes were transferred into free-TSA culture medium for 3 hour later.The conclusion could be made that there isn＇t activity to HAT,but HDAC was activity during reduction division phase of Ⅰand Ⅱ.

In the study,the aim was to investigate the expression of Hairless gene in Inner Mongolia goat.The degenerate primers are designed based on the known conserved sequence of Hairless gene,after the genome DNA was extracted from blood,then the aim gene was amplified by PCR technique,the product of PCR was sequenced.The result indicated that the gene was about 1 985 bp,including 3 exons and 2 introns,and the result of blast also showed it was specific compared with that of cow and sheep in GenBank.

With the limitation in conventional energy and the increasingly serious environmental pollution,it necessary to find some more clean regeneration energy.Because of the large quantity,low effect to environment and the reproducible ability,plant biomass chemical technology became one of the main selected energy,therefore it can be paid attention for its high valued liquid and gas products that can be used for effective clean energy.In the article,the chemical technology progress of plant biomass energy combustion and four ways of these technologies are introduce,including combustion,pyrolysis,liquefaction and gasification.

The embryonic diseases of chicken refer to those diseases which occur in the process of embryo development.The effective control of chicken＇s embryonic diseases has great significance for improvement of the fertilization rate,hatching rate,healthy chick rate,as well as the productivity and raising efficiency of chicken.In the article,the cause of the diseases,the types and the prevention and treatment measures of chicken＇s embryonic diseases were reviewed.

Sustainable development of agriculture is the goal of modern agriculture,biological nitrogen fixation is one of the most effective way.Nowadays,the Frankia nitrogen fixation advantage was paid more attention in agricultural production for its unique.In the article,the Frankia application in agricultural production of the current situation,prospects and some problems were reviewed.

As one of the important tree species for afforestation of ＇Three-North＇ in China,Seabuckthorn has strong adaptability,anticold wind and sand,drought and heat resistant to the atmosphere.It can improve soil,sand-fixing,maintaining soil and water,is a good firewood species.It is very high economic value,because Seabuckthorn is a multi-vitamin natural base of plants is a source of good drinks.In addition,Seabuckthorn Oil contains some multi-vitamins and all kinds of living matter concentrates.Seabuckthorn fruit not only has a high nutritional value,but also the value of superior medicine,which can treat many diseases,conservation of the skin.Asexual reproduction form of Seabuckthorn is sub-hardwood cuttings,softwood cuttings,root tillering breeding etc.