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Table of Content
30 July 2023, Volume 44 Issue 4
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  • Special Column:Scientific and Technological Innovation Supporting High-quality Development of Animal Husbandry A Series of Articles on High-quality Development of Cashmere Goat Industry in Ordos City, Inner Mongolia ( 1 )
    Strengthening ′Big Data+′ Based Science and Technology Service to Promote High-quality Development of Cashmere Goat Industry-A Case Study of Ordos City, Inner Mongolia
    Siqinmenghe , LI Shengli, SUN Haizhou, ZHANG Chunhua, ZHANG Le, ZHANG Yanning, BAI Yu
    2023, 44(4):  1-9.  doi:10.12160/j.issn.1672-5190.2023.04.001
    Abstract ( 108 )   HTML( 36 )   PDF (1296KB) ( 36 )   Save
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    [Objective] The present study was conducted to analyze the roles and achievements of the ′big data+′ based science and technology service model in promoting the high-quality development of the cashmere goat industry in Ordos City, Inner Mongolia. [Method] Based on the implementation of the ′Science and Technology Revitalizing Inner Mongolia′ project undertaken by the authors′ research team, empirical analysis methods were used to analyze the construction content and operation status of the big data platform for the cashmere goat industry in Ordos City, Inner Mongolia, and to explore the roles of the cashmere goat production technology service team. Furthermore, the science and technology support mechanisms for the high-quality development of the cashmere goat industry were constructed, and some suggestions for the extending development were proposed. [Result] According to the architecture of ′one platform, four systems, and one center′, a big data platform for the cashmere goat industry in Ordos City was established, and a socialized service system for the cashmere goat production technology as well as a digital development management system for the cashmere goat industry were preliminary constructed. Based on the implementation of ′Science and Technology Revitalizing Inner Mongolia′ project, using the advantages of scientific research resources from universities and research institutes, the leading organizer of the project led the private enterprises and the professional cooperatives of cashmere goat farming in this region formed a ′1+4+10+N′ socialized service model for the cashmere goat production technology. The services included guidance and training on farming technology, veterinary medicine and veterinary drugs, breed selection and promotion, artificial insemination, forage guarantee, cashmere harvest by parts and graded packaging, infrastructure construction in agriculture and animal husbandry, and so on. On the basis of the internal mechanisms of the established big data platform and the socialized service model, six aspects of the science and technology support mechanisms were constructed. In addition, some suggestions were put forward, such as advancing ′the reforms to streamline administration, delegate powers, improve regulation and strengthen services′, accelerating the construction of digital farm, and promoting the diversified science and technology support. [Conclusion] Through the implementation of ′Science and Technology Revitalizing Inner Mongolia′ project, the the ′big data+′ based science and technology service model in the high-quality development of the cashmere goat industry in Ordos City has been basically established. Furtherly, the socialized service system for the cashmere goat production technology and the management system for the digital transformation and development of the cashmere goat industry should be continuously improved, the construction of big data platforms and technical service stations extending to the grassroots level should be encouraged and supported, the digital transformation and development of the cashmere goat industry as well as the full coverage of socialized services should be promoted, so as to continuously accelerate the high-quality development of the cashmere goat industry in this region.

    Basic Research
    Bioinformatics Analysis on the Structure and Function of African Swine Fever Virus pI215L Protein
    ZHAO Jinbing, WANG Tiejun, WANG Moli, SHI Jianzhou, YAO Lunguang
    2023, 44(4):  10-16.  doi:10.12160/j.issn.1672-5190.2023.04.002
    Abstract ( 1268 )   HTML( 76 )   PDF (1495KB) ( 76 )   Save
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    [Objective] This study aimed to analyze the structure and function of African swine fever virus (ASFV) pI215L protein. [Method] A total of 29 pI215L gene sequences of different ASFV strains were downloaded from the African Swine Fever Virus Database (ASFVdb). Bioinformatics tools were used to analyze the homology and genetic evolution of pI215L gene of different ASFV strains, and to predict the physicochemical properties, secondary structure, linear epitope, and three-dimensional spatial location of pI215L protein. [Result] The pI215L gene sequences had high homology among different ASFV strains. The pI215L protein was unstable and hydrophilic. Its secondary structure mainly contained four forms, including α-helix, extend strand, β-turn, and random coil, and had a domain of E2 Ubiqutin-conjugating enzyme. A total of 16 peptide fragments were potential linear epitopes. SWISS-MODEL was used to complete homology modeling of the tertiary structure of pI215L protein, and its homology with 6NYO.1 (ubiquitin-conjugating enzyme E2 R2 and ubiquitin) in the PDB database was 46.06%. The PyMOL software was used to predict the conformational epitopes of pI215L protein to accurately locate the distribution of the predicted three-dimensional spatial position of the epitopes in the model. [Conclusion] Our results provided references for unravelling the structure and function of pI215L protein, as well as developing vaccines and drugs for prevention and control of ASFV.

    Expression of Caspase-9 Protein in Leydig Cells of Rats with Sub-chronic Arsenic Poisoning
    QIN Haixia, HAN Fei, DAI Yanping, LIU Gaiping
    2023, 44(4):  17-21.  doi:10.12160/j.issn.1672-5190.2023.04.003
    Abstract ( 92 )   HTML( 37 )   PDF (2550KB) ( 37 )   Save
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    [Objective] This study was conducted to investigate the effects of sub-chronic arsenic poisoning on the expression of Caspase-9 protein in leydig cells of rats. [Method] A total of 40 healthy adult clean-grade male SD rats were randomly assigned into the following 4 groups:a control group and the low-, medium-, and high-dose arsenic exposure groups, with 10 rats in each group. The rats in the low-, medium-, and high-dose arsenic exposure groups were given sodium arsenate by drinking water at concentrations of 2.4, 12 and 60 mg/L, respectively, and those in the control group was given physiological saline. The exposure test lasted for 14 weeks. Following the exposure test, the number of peripheral white blood cells of the rats was counted; the serum levels of TNF-α and IL-1 were measured by ELISA assay; the ultrastructure of sperm was observed using transmission electron microscopy; the expression level and localization of Caspase-9 protein in leydig cells were assessed by immunohistochemical method. [Result] Compared with the control group, the serum levels of TNF-α and IL-1 as well as the number of peripheral white blood cells in the medium- and high-dose arsenic exposure groups significantly (P<0.05) increased. Under transmission electron microscopy, the sperm acrosome structure in the control group was intact, with uniform thickness and staining. In the low-dose arsenic exposure group, the sperm membrane swelled and the nuclear chromatin was uniform. In the medium-dose arsenic exposure group, the sperm acrosome and nucleus split, and the membrane swelled. In the high-dose arsenic exposure group, the sperm tail disrupted and the axial filament arrangement disordered. Immunohistochemical analysis showed that Caspase-9 protein mainly expressed in the cytoplasm of leydig cells, and the expression signal was dark brown. Compared with the control group, the expression level of Caspase-9 protein in the testicular tissue in all the arsenic exposure groups significantly (P<0.05) increased. [Conclusion] The increased expression level of Caspase-9 protein in laydig cells may be one of the mechanisms underlying chronic reproductive toxicity induced by sodium arsenite in male rats.

    Effects of Citric Acid and Acetic Acid on the Contraction of Isolated Intestinal Smooth Muscle in Mice
    ZHOU Xinyi, FAN Zhen, YUE Jiatian, LIU Xinbo, ZHAO Hongqiong
    2023, 44(4):  22-28.  doi:10.12160/j.issn.1672-5190.2023.04.004
    Abstract ( 140 )   HTML( 43 )   PDF (922KB) ( 43 )   Save
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    [Objective] This study was conducted to explore the effects and possible mechanisms of citric acid and acetic acid on the contraction of isolated mouse intestinal smooth muscle, and to provide references for the development of organic acid products that helps regulate gastrointestinal motility disorders in humans or animals. [Method] A biological signal processing system was used to monitor the effects of two-fold increasing concentrations of citric acid (0.02-0.64 mmol/L) or acetic acid (0.13-2.00 mmol/L) as well as their corresponding bolus effective concentrations on the contractile amplitude, ension, and peak of the isolated intestinal segment in mice. [Result] Both citric acid and acetic acid dose-dependently reduced the contractile amplitude and tension of the intestinal smooth muscle (P<0.01), with the effective inhibitory concentrations of 0.32 mmol/L and 1.00 mmol/L, respectively. Both acids with effective inhibitory concentrations did not affect the contractile peak of the intestinal tissue caused by 25 mmol/L KCl (P>0.05), while the citric acid treatment significantly (P<0.05) alleviated the decrease in intestinal contractile amplitude induced by bethanechol chloride, an M-receptor agonist. [Conclusion] Both citric acid and acetic acid can inhibit the contraction of isolated intestinal smooth muscle in mice, and may not affect the depolarization process of smooth muscle cell membrane. However, citric acid may have spasmolytic effect on gastrointestinal smooth muscle via partially affecting the role of M-receptor.

    Research Progress in Molecular Regulatory Mechanisms in the Development of Non-alcoholic Fatty Liver Disease and Its Transition to Liver Cancer
    BIAN Kangkun, BAO Yulong, WANG Li
    2023, 44(4):  29-40.  doi:10.12160/j.issn.1672-5190.2023.04.005
    Abstract ( 104 )   HTML( 33 )   PDF (1923KB) ( 33 )   Save
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    Inflammation and fibrosis are important signs in the development of non-alcoholic fatty liver disease (NAFLD). There are multiple signaling pathways that exert key roles in the progression of NAFLD and its transition to liver cancer, regulating further damage and malignant transition of hepatocytes. Among them, the Hippo signaling pathway controls the progression of NAFLD by regulating lipid metabolism and activating hepatic stellate cells (HSC); the activation of the PI3K/AKT signaling pathway can inhibit the expression of inflammatory factors, enhance anti-inflammatory capabilities, and reduce the activity of inflammatory factors in the liver, thereby alleviating the degree of hepatocyte damages; the TGF-β signaling pathway aggravates non-alcoholic steatohepatitis (NASH) by further promoting hepatocyte apoptosis and HSC activation under chronic inflammatory conditions; the AMPK signaling pathway plays important roles in carbohydrate and fat metabolism regulation, energy regulation and the transition from NAFLD to liver cancer; the NF-κB signaling pathway regulates the expression of pro-inflammatory genes, and the process of steatohepatitis and liver fibrosis can be reduced by inhibiting this pathway. This paper presents a review on the research progress in the regulatory mechanisms of the main signaling pathways involved in the development of NAFLD and its transition to liver cancer, in hoping to prevent further lesions and transition to liver cancer in the early stage of NAFLD as much as possible, and to provide references for targeted therapy of NAFLD in clinical practice.

    Animal Nutrition and Feed Science
    Effects of Partly Replacing Whole-plant Corn Silage with Quinoa Straw Micro-silage on Production Performance, Nutrient Apparent Digestibility, and Serum Indexes of Fattening Simmental Crossbred Cattle
    ZHANG Junli, BAI Shilin, HOU Pengxia, LIANG Xiaojun
    2023, 44(4):  41-46.  doi:10.12160/j.issn.1672-5190.2023.04.006
    Abstract ( 1218 )   HTML( 33 )   PDF (849KB) ( 33 )   Save
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    [Objective] This study was conducted to assess the effects of partly replacing whole-plant corn silage with quinoa straw micro-silage on production performance, nutrient apparent digestibility, and serum indexes of fattening Simmental crossbred cattle. [Method] A total of 36 Simmental crossbred cattle with good physical condition and initial weight of around 450 kg were selected and randomly assigned into the following 4 groups: a control group and three experiment groups. There were 9 cattle in each group, and each head was a replicate. The control group fed a basal diet (TMR), and quinoa straw micro-silage was used to replace whole-plant corn silage at ratios of 10%, 30%, and 50% on the basis of roughage in the basal diet in experiment group 1, experiment group 2, and experiment group 3, respectively. The pre-feeding period was 15 d, and the formal feeding period was 60 d. The body weight and feed intake status of the experimental beef cattle in each group during the experiment period was recorded to calculate the average daily gain, average daily feed intake, and average feed to gain ratio. Diet and fecal samples were collected to determine the contents of main nutrients, and to calculate the nutrient apparent digestibility. At the end of the feeding experiment, blood were collected to prepare serum samples, and the serum biochemical indexes, antioxidant capacity indexes, and immune indexes were measured. [Result] Compared with the control group, the experiment group 1 had significantly (P<0.05) increased average daily gain, while had significantly (P<0.05) reduced average feed to gain ratio. The apparent digestibilities of dry matter (DM), crude protein (CP), crude fiber (CF), neutral detergent fiber (NDF), and acidic detergent fiber (ADF) were significantly lower (P<0.05) in experiment group 1, experiment group 2, and experiment gruop 3 than those in the control group. In experiment group 1, the serum activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px), the total antioxidant capacity (T-AOC), as well as the contents of IgG and IgM were significantly (P<0.05) higher than those in the control group, while the serum level of MDA was significantly (P<0.05) lower than that in the control group. [Conclusion] Partly replacing whole-plant corn silage with quinoa straw micro-silage in the diet of fattening Simmental crossbred cattle increased the daily gain, reduced feed to gain ratio, and enhanced the antioxidant and immune ability. Substitution at a ratio of 10% was recommended.

    Research Progress in Common Mycotoxins Contamination in Feed and the Toxic Effects on Livestock and Poultry
    LIU Qiujin, YIN Junyi, ZHANG Jun, WANG Yan, BAI Changsheng, TIAN Qiufeng, WANG Huan, XUE Zhanmei, MIAO Yan
    2023, 44(4):  47-54.  doi:10.12160/j.issn.1672-5190.2023.04.007
    Abstract ( 245 )   HTML( 128 )   PDF (938KB) ( 128 )   Save
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    Mycotoxins are secondary metabolites produced by fungi in grains and are often present in the contaminated livestock and poultry feed and feed raw materials. The mycotoxins commonly found in the feed samples include aflatoxin B1(AFB1), ochratoxin A (OTA), zearalenone (ZEA), deoxynivalenol (DON), and fumonisin (FUM). Mycotoxins damage the immune system and reproductive function of livestock and poultry, and cause pathological alterations in the organs such as kidney, liver, and intestinal tract, affecting the health of livestock and poultry. This paper introduces the types and contamination situation of the commonly observed mycotoxins in livestock and poultry feed and feed raw materials, summarizes the research progress in the toxic effects of mycotoxins on livestock and poultry, and proposes corresponding prevention and control measures, in hoping to provide references for minimizing the harm caused by mycotoxin contamination in livestock and poultry production.

    Animal Genetics, Reproduction and Breeding
    Research Progress in Candidate Genes Related to Meat Quality Traits of Livestock and Poultry
    YANG Hang, LIU Xu
    2023, 44(4):  55-60.  doi:10.12160/j.issn.1672-5190.2023.04.008
    Abstract ( 152 )   HTML( 76 )   PDF (736KB) ( 76 )   Save
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    The meat quality traits of livestock and poultry have become a research hotspot in the fields of food science and animal breeding due to the rising market demand for high-quality livestock and poultry meat. Meat quality traits are affected by multiple signaling pathways and transcription factors. The thorough understanding of the candidate genes that regulate meat quality traits is important for improving meat quality and breeding superior livestock and poultry breeds. This paper reviews the research progress in candidate genes closely related to intramuscular fat (IMF) content, inosine monophosphate (IMP) content, tenderness, pH value, color, and other meat quality traits, in hoping to provide theoretical guidance for investigations on livestock and poultry breed improvement and molecular marker assisted breeding.

    Animal Husbandry Resource and Environment
    Spatial-temporal Characteristics of Carbon Emissions from Livestock and Poultry Industry and Emission Reduction Paths: A Case Study of Henan Province
    KONG Xiangfei,ZHANG Dongsheng
    2023, 44(4):  61-69.  doi:10.12160/j.issn.1672-5190.2023.04.009
    Abstract ( 124 )   HTML( 28 )   PDF (893KB) ( 28 )   Save
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    [Objective] This study analyzed the spatial-temporal characteristics of carbon emissions from livestock and poultry industry in Henan Province, proposed the countermeasures and suggestions on emission reduction paths, so as to provide references for accelerating the high-quality development of livestock and poultry industry in this province. [Method] The carbon emissions from livestock and poultry industry in Henan Province were taken as the research objects. Based on the total amount of cattle, horses, donkeys, mules, pigs, sheep and poultry for sale as well as the output value of livestock and poultry industry in Henan Province and its sub-administrative regions (17 prefecture-level cities and 1 county-level city directly under the province government) from the year of 2011 to 2020, and using CH4 and N2O produced by the intestine, stomach and excreta of various livestock and poultry as the source of greenhouse gases, the spatial-temporal characteristics of the total carbon emission equivalent and the carbon emission intensity of livestock and poultry industry in Henan Province and its sub-administrative regions were assessed by the carbon emission factor method and the carbon emission intensity calculation formula of livestock and poultry industry. [Result] In terms of the total carbon emission equivalent, the livestock and poultry industry and its various internal structures in Henan Province showed an overall downward trend from the year of 2011 to 2020. In terms of the year-on-year growth rate of the total carbon emission equivalent, the absolute carbon emission equivalent of livestock and poultry industry in Henan Province had gone through three stages with different fluctuation characteristics: a small increase stage, a significant decrease stage, and a fluctuation change stage. According to the characteristics of the absolute carbon emission equivalent of livestock and poultry industry, the sub-administrative regions of Henan Province could be categorized into five types: ′high-fluctuation′, ′medium-fluctuation′, ′medium-decline′, ′low-fluctuation′, and ′low-decline′. In terms of the absolute carbon emission intensity equivalent, the overall carbon emission intensity of livestock and poultry industry in Henan Province showed a downward trend from the year of 2011 to 2020, and the economic development quality of livestock and poultry industry improved greatly. In terms of the year-on-year growth rate of carbon emission intensity, the carbon emission intensity of livestock and poultry industry in Henan Province had undergone a fluctuating decline process, which was divided into two stages: a fluctuating decline stage and a fluctuating rise stage. According to the characteristics of the carbon emission intensity of livestock and poultry industry, the sub-administrative regions of Henan Province could be divided into 8 types: ′high-convergence growth′, ′high-fluctuation′, ′high-expansion decline′, ′medium-fluctuation′, ′medium-expansion decline′, ′medium-convergence decline′, ′low-fluctuation′, and ′low-convergence decline′. [Conclusion] Carbon emission reduction in livestock and poultry industry in Henan Province had made some progress in terms of quantity and quality. The obtained achievements in carbon emission reduction were unstable. The total carbon emission equivalent and the carbon emission intensity were characterized by regional heterogeneity. It is recommended that Henan Province achieve the modernized development of livestock and poultry industry through the following paths: strengthening the comprehensive utilization of livestock and poultry manures, enhancing the investment and application of science and technology in carbon emission reduction in livestock and poultry industry, optimizing the management practices of livestock and poultry, cooperatively promoting the greenhouse gas reduction through multiple policies, and accelerating the integrated development of the entire industry chain of livestock and poultry industry.

    Comparative Study on Soil Organic Carbon Contents and the Influencing Factors of Five Types of Alpine Deserts in Qinghai Province
    WANG Dinghui, WANG Xin, WANG Yunying, PEI Weiwei, DU Yangong
    2023, 44(4):  70-75.  doi:10.12160/j.issn.1672-5190.2023.04.010
    Abstract ( 86 )   HTML( 21 )   PDF (1388KB) ( 21 )   Save
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    [Objective] This study was conducted to assess the soil organic carbon (SOC) contents of five types of alpine deserts and to reveal their regulatory factors. [Method] The alpine desert ecosystems of Qaidam Basin and Gonghe Basin in Qinghai Province were selected as the research objects. A total of 221 soil profile samples were collected using surface investigation method to analyze their SOC contents. In addition, structural equation model was used to reveal the main influencing factors of SOC content. [Result] The SOC contents of the five types of alpine deserts in Qinghai Province exhibited strong heterogeneity. The highest SOC content was observed in chestnut soil, followed by saline soil, brown calcic soil, grey-brown desert soil, and aeolian sandy soil. The SOC contents in chestnut soil and saline soil were significantly (P<0.05) higher than the other three soil types. The pH value of the alpine desert soils had weak heterogeneity, with the order from high to low being aeolian sandy soil, chestnut soil, brown calcic soil, grey-brown desert soil, and saline soil. The SOC content of the alpine deserts was mainly affected by total soil nitrogen content and precipitation, while the pH value was mainly regulated by annual average temperature. [Conclusion] The SOC contents of the alpine deserts in Qinghai Province was relatively higher and had strong heterogeneity, which was mainly attributed to the input of carbon sources from plant roots.

    Smart Animal Husbandry
    Establishment of an A-Unet Based Cattle Body Size Measurement Method
    SHI Wei, ZHANG Xianyu, YANG Jing′an, ZHAO Yan
    2023, 44(4):  76-84.  doi:10.12160/j.issn.1672-5190.2023.04.011
    Abstract ( 242 )   HTML( 80 )   PDF (3236KB) ( 80 )   Save
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    [Objective] This study aimed to establish an improved A-Unet image segmentation and cattle body size measurement method on the basis of U-Net to achieve the automated measurement of cattle body height, body length, and body oblique length. [Method] Firstly, side view images of the farm-raised cattle were collected through cameras. Secondly, the A-Unet algorithm was used for image segmentation to extract the contour curve of cattle body edge. Based on the contour curve of cattle body, the dynamic grid method was adopted to find the cattle body size measurement points. Finally, according to the calibrated parameters by cameras and the extracted measurement points, the cattle body size data was calculated. [Result] The established A-Unet algorithm was found to have higher accuracy than the original U-Net algorithm through comparative analysis of the image segmentation performance of deep learning algorithms. Compared with the manual measurement, the average relative errors of the body height, body length and body oblique length of 21 farm-raised cattle measured by the established A-Unet algorithm were 4.16%, 4.05% and 4.27%, respectively. [Conclusion] With the advantages of good applicability, high stability and high detection accuracy, the A-Unet based cattle body size measurement method could effectively replace the traditional manual measurement method. The measurement error met the needs of herdsmen for measuring cattle body size.

    Pratacultural Science
    Effects of Nitrogen Fertilizer Application Rates on Contents of Protein and Cellulose in Forage Oat
    WANG Jing, TIAN Yonglei, MU Zongjie, WANG Yongrong, BAI Chunli
    2023, 44(4):  85-93.  doi:10.12160/j.issn.1672-5190.2023.04.012
    Abstract ( 120 )   HTML( 27 )   PDF (1066KB) ( 27 )   Save
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    [Objective] This study aimed to assess the effects of nitrogen fertilizer application rates on the quality of forage oat, and to determine the optimal range of nitrogen fertilizer application rate for forage oat. [Method] Using oat grass as the experimental material, a total of 7 nitrogen fertilizer application gradients were set up, including 0 (N0), 175 (N175), 200 (N200), 225 (N225), 250 (N250), 275 (N275), and 300 (N300) kg/hm2. At the seedling stage, tillering stage, jointing stage, and heading stage, 15%, 35%, 30%, and 20% nitrogen fertilizer topdressing were applied, respectively. A single factor randomized block design was adopted in the plots of the field trial, with 3 replicates in each block. At the jointing stage and heading stage, the alkali-hydrolyzed nitrogen content in the 0-10 cm, 10-20 cm, and 20-30 cm soil layers, as well as the contents of protein and cellulose of oat grass were measured, respectively. [Result] With the increase of nitrogen fertilizer application rates, the soil alkali-hydrolyzed nitrogen content elevated, and an accumulation phenomenon was observed. Compared with N0, the alkali-hydrolyzed nitrogen content in each soil layer of the different nitrogen fertilizer application rates at both jointing stage and heading stage increased in various degrees. The quality of forage oat did not continuously improve with the increase of available nutrient content of nitrogen, and had a peak. At the nitrogen fertilizer application rate of N225, the protein content of oat grass peaked at both jointing stage and heading stage, reaching 11.55% and 16.13% respectively, with an increase of 38.15% and 53.62%, respectively. The highest tillering number of forage oat was observed at the nitrogen fertilizer application rate of N175 (median=8). The contents of protein and cellulose of oat grass exhibited a trend of first increasing and then decreasing with the elevation of nitrogen fertilizer application rates. At the nitrogen fertilizer application rates of N225 and N175, the contents of protein and cellulose reached the maximum values, respectively. [Conclusion] Within the range of nitrogen fertilizer application rate at N225-N275, the contents of protein and cellulose of forage oat reached the synergistic optimal value.

    Effects of Exogenous Brassinosteroids Priming on Germination Characteristics of Aged Oat Seeds
    LI Yinlin, WANG Bo, XIA Fangshan, ZHANG Jiemin, ZHANG Jincheng, LI Jiajun
    2023, 44(4):  94-100.  doi:10.12160/j.issn.1672-5190.2023.04.013
    Abstract ( 131 )   HTML( 16 )   PDF (696KB) ( 16 )   Save
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    [Objective] The aim of the present study was to explore the effects of exogenous brassinosteroids (BR) priming on aged oat (Avena sativa) seed vigor. [Method] Artificially aged oat seeds were used as experimental materials and were primed with different concentrations (0, 0.01, 0.10, 0.50, 1.00, and 10.00 μmol/L) of BR solution for 0 (CK), 1, 6, 12, 18, and 24 h, respectively. The changes in the germination percentage, germination index, mean germination time, and seedling vigor index were characterized. [Result] Different concentrations of exogenous BR, priming time and their interaction had extremely significant (P<0.01) effects on germination percentage, germination index, mean germination time, and seedling vigor index of the aged oat seeds. The aged oat seeds exhibited the highest germination rate, germination index, seedling vigor index as well as the lowest mean germination time when they were primed with BR at the concentration of 0.01 μmol/L for 24 h. After BR priming for 1-24 h at the concentration of 10.00 μmol/L, the germination rate, germination index, and seedling vigor index of the aged oat seeds were all lower than the other concentrations, while the mean germination time was significantly (P<0.05) higher than the other concentrations. [Conclusion] Low concentration of exogenous BR priming could increase the aged oat seed vigor, while high concentration exhibited the opposite effect. Under the experimental conditions in this study, the optimal treatment to alleviate the oat seed aging was priming with BR for 24 h at a concentration of 0.01 μmol/L.

    Food Science
    Determination and Evaluation on Visceral Selenium Content in Hulun Buir Grassland Short-tailed Sheep in Inner Mongolia
    ZHANG Limei,GUO Jun,YE Le,LIU Yujia,LIU Xiaoqi,GUO Lingzhi
    2023, 44(4):  101-108.  doi:10.12160/j.issn.1672-5190.2023.04.014
    Abstract ( 105 )   HTML( 12 )   PDF (1045KB) ( 12 )   Save
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    [Objective] Hulun Buir grassland short-tailed sheep is an excellent breed of Mongolian sheep announced by the Ministry of Agriculture and Rural Affairs at the end of 2020. This paper performed systematic detection and evaluation of visceral selenium (Se) content in this sheep breed. [Method] A total of 170 visceral samples, including heart, lung, kidney, liver, small intestine, rumen, reticulum, and abomasum, were collected from 23 grassland short-tailed sheep reared in Ewenki grassland in Hulun Buir City, Inner Mongolia. The visceral Se content was determined by atomic fluorescence spectroscopy and the obtained data were subjected to statistical analysis. [Result] The highest visceral Se content (X±SD, μg/100 g) of the grassland short-tailed sheep was observed in kidney (47.26±19.18), followed by lung(6.30±2.94), small intestine (6.16±2.26), heart (5.63±3.82), liver (4.49±2.52), reticulum (2.96±2.03), rumen (2.84±1.40), and abomasum (2.44±1.81). The Se content of kidney was 7.5-19.4 times higher than that of the other visceral organs.The mean value of the Se content in substantive organs was extremely significantly (P<0.01) different from that in gastrointestinal tract. The visceral Se content exhibited a correlation with the age of the grassland short-tailed sheep. In general, higher visceral Se contents were found in 30- and 18-month-old sheep in comparison to 6- and 12-month-old sheep. The Se contents of kidney, lung, small intestine, heart, rumen, and abomasum were significantly (P<0.05) different among the four age groups. The most obvious age-related difference of visceral Se content was observed in kidney, and the Se content in kidney in 30-, 18-, 42-month-old sheep was 2.1-2.5 times higher than that in 6-month-old sheep. The visceral Se content was generally higher than that of muscle. [Conclusion] The viscera of Hulun Buir grassland short-tailed sheep have a significant effect of Se bioaccumulation. Kidney of this sheep breed is Se-rich food, and the other viscera are also Se-rich or Se-containing foods. Hulun Buir City is located in the Se shortage area in Northeast China. It is recommended to attach more importance to Se supplementation or Se-rich feeding of grassland short-tailed sheep in a scientific manner, which can make mutton and viscera more nutritious and healthy.

    Research Progress in Quality and Safety Evaluation of Asini Corii Colla
    KONG Hao, TIAN Rufang, CAO Guiyun, MENG Zhaoqing
    2023, 44(4):  109-115.  doi:10.12160/j.issn.1672-5190.2023.04.015
    Abstract ( 79 )   HTML( 49 )   PDF (1069KB) ( 49 )   Save
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    As a medicine and food homologous product, Asini Corii Colla has a long history of application in China. The market demands are steadily rising as its nourishing and health-preserving functions are favored by more and more consumers. Due to insufficient supply of raw materials and imperfect evaluation methods for product quality and safety, the quality of Asini Corii Colla product in the market varies. The main issue is actually an industrial chain problem, involving various fields such as farming, production, storage and transportation. In this paper, the research progress in quality and safety evaluation of Asini Corii Colla is summarized from the aspects of raw material control, production and circulation, and the problems in each link of the industrial chain are analyzed, in hoping to provide references for the high-quality development of Asini Corii Colla industry.

    Research Progress in Protective Effects of Camel Milk on Different Types of Liver Injury
    GAO Tian, DU Donghua, Hasisurong
    2023, 44(4):  116-121.  doi:10.12160/j.issn.1672-5190.2023.04.016
    Abstract ( 92 )   HTML( 17 )   PDF (712KB) ( 17 )   Save
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    Liver injury threatens human health and life safety, which has various causative factors, such as drugs, alcohol, diabetes mellitus, and environmental stress. In animals, liver injury leads to decrease in production performance and results in severe economic losses in animal husbandry production. Oxidative stress (OS) and its mediated inflammatory response are the common pathogenesis of different types of liver injury. Camel milk (CM) and camel whey protein (CWP) are natural antioxidants that can prevent OS by boosting the antioxidant capacity of the body. Therefore, they have potential anti-liver damage effects. Focusing on the roles of anti-OS and anti-inflammation, the research progress in the protective effects of CM and CWP against the chemical, alcoholic and diabetes mellitus-induced liver injury as well as the heat stress-induced liver injury is reviewed, in hoping to give novel insights into the future research and provide references for promoting the health care role of CM.

    Animal Disease Prevention and Control
    Molecular Identification and Genetic Diversity of Echinococcus granulosus Derived from Sheep in Abattoirs in Parts of Xinjiang Uygur Autonomous Region,China
    XUE Wen, WANG Lingyun, WANG Yunfeng, ZHANG Zhenjie, ZHAO Aiyun, WANG Tian, QI Meng
    2023, 44(4):  122-128.  doi:10.12160/j.issn.1672-5190.2023.04.017
    Abstract ( 1229 )   HTML( 19 )   PDF (1854KB) ( 19 )   Save
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    [Objective] The aim of this study was to understand the infection status and molecular genetic characteristics of Echinococcus granulosusE. granulosus) derived from sheep in some large-scale abattoirs in Xinjiang Uygur Autonomous Region, China. [Method] A total of 96 cyst samples identified as E. granulosus by macroscopic observation were collected from the liver tissues of sheep in abattoirs. Following genomic DNA extraction, PCR amplification and sequencing were performed based on the cox1 and nad1 gene loci of E. granulosus. Sequence alignment was carried out to identify the genotype. The genetic evolution tree was constructed to analyze the molecular genetic characteristics. [Result] At the cox1 gene locus, a total of 81 samples were positive in PCR amplification (84.37%, 81/96). Sequence analysis identified that all positive samples were G1 genotype of E. granulosusn=81), with 15 haplotypes (Hap_1-15) presented. At the nad1 gene locus, there were 67 PCR positive samples (69.79%, 67/96). Two genotypes, G1 genotype (n=66) and G3 genotype (n=1) of E. granulosus, were identified through sequence analysis,with 19 haplotypes (Hap_1-19) presented. According to the phylogenetic analysis, the G1 and G3 genotypes of E. granulosus obtained in this study were in the same evolutionary branches as their counterparts of E. granulosus derived from the multiple host sources reported in China and abroad. [Conclusion] The E. granulosus derived from sheep in some parts of Xinjiang showed genetic diversity. Our results provide fundamental data for the prevention and control of echinocococosis in sheep in this region.

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(Published monthly,Science 1973)
Responsible Unit:Inner Mongolia Department of Agriculture and Animal Husbandry
Sponsor Unit:Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences
International standard serial number:ISSN 1672-5190
Domestic unified serial number:CN 15-1228/S
CODEN XYSKAX
Domestic postal distributing code:16-101

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