新疆部分地区屠宰场羊源细粒棘球蚴分子鉴定及其遗传多样性分析

doi:10.12160/j.issn.1672-5190.2023.04.017

Abstract

Abstract:

[Objective] The aim of this study was to understand the infection status and molecular genetic characteristics of Echinococcus granulosus （E. granulosus） derived from sheep in some large-scale abattoirs in Xinjiang Uygur Autonomous Region, China. [Method] A total of 96 cyst samples identified as E. granulosus by macroscopic observation were collected from the liver tissues of sheep in abattoirs. Following genomic DNA extraction, PCR amplification and sequencing were performed based on the cox1 and nad1 gene loci of E. granulosus. Sequence alignment was carried out to identify the genotype. The genetic evolution tree was constructed to analyze the molecular genetic characteristics. [Result] At the cox1 gene locus, a total of 81 samples were positive in PCR amplification （84.37%, 81/96）. Sequence analysis identified that all positive samples were G1 genotype of E. granulosus （n=81）, with 15 haplotypes （Hap_1-15） presented. At the nad1 gene locus, there were 67 PCR positive samples （69.79%, 67/96）. Two genotypes, G1 genotype （n=66） and G3 genotype （n=1） of E. granulosus, were identified through sequence analysis,with 19 haplotypes （Hap_1-19） presented. According to the phylogenetic analysis, the G1 and G3 genotypes of E. granulosus obtained in this study were in the same evolutionary branches as their counterparts of E. granulosus derived from the multiple host sources reported in China and abroad. [Conclusion] The E. granulosus derived from sheep in some parts of Xinjiang showed genetic diversity. Our results provide fundamental data for the prevention and control of echinocococosis in sheep in this region.

Key words: Echinococcus granulosus, detection, genotype, haplotype, sheep

CLC Number:

XUE Wen, WANG Lingyun, WANG Yunfeng, ZHANG Zhenjie, ZHAO Aiyun, WANG Tian, QI Meng. Molecular Identification and Genetic Diversity of Echinococcus granulosus Derived from Sheep in Abattoirs in Parts of Xinjiang Uygur Autonomous Region，China[J]. Animal Husbandry and Feed Science, 2023, 44(4): 122-128.

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Molecular Identification and Genetic Diversity of Echinococcus granulosus Derived from Sheep in Abattoirs in Parts of Xinjiang Uygur Autonomous Region，China

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