关键词: 沂水食用百合, 快繁体系, 组织培养

Abstract: 【Objective】 To establish tissue culture and rapid propagation system of Yishui edible Lilium brownii var. viridulum.【Methods】 The bulbs of Yishui edible Lilium brownii var. viridulum N6 were used as the experimental materials and MS medium was selected as the basic medium,and the tissue culture methods were used to induce buds in different parts of Yishui edible Lilium brownii var. viridulum explants,and the effects of different concentrations of plant growth regulators（6-BA,NAA,IBA）on adventitious bud induction,proliferation and rooting were analyzed and select the most suitable transplanting substrate.【Results】 The scales in the middle of the inner layer bulbs of Yishui edible Lilium brownii var. viridulum were selected as the highest explant induction rate,which was 55.56%,and the pollution rate was lower,which was 40.00%. The best adventitious bud induction medium was 1.0 mg/L NAA+1.0 mg/L 6-BA+MS,the induction rate was 96.67%;the best proliferation medium was 0.1 mg/L NAA+0.5 mg/L 6-BA+MS,the proliferation coefficient was 3.67,its adventitious buds grow in clusters,the seedlings were strong,and the plant growth was the best. The best rooting medium was 0.5 mg/L NAA+0.3 mg/L IBA+MS,the rooting rate was 93.33%,and the roots were strong and thick. The best transplanting substrate was domestic grass charcoal soil+sphagnum moss,the survival rate was 90%,and the average plant height was 7.75 cm.【Conclusion】 The best adventitious bud induction medium for obtaining Yishui edible Lilium brownii var. viridulum scale tissue culture was 1.0 mg/L NAA+1.0 mg/L 6-BA+MS,and the best proliferation medium was 0.1 mg/L NAA+0.5 mg/L 6-BA+MS,the best rooting medium was 0.5 mg/L NAA+0.3 mg/L IBA+MS,and the best transplanting substrate was domestic grass charcoal soil+sphagnum moss.

Key words: Yishui edible Lilium brownii var. viridulum, Rapid propagation system, Tissue culture