SBPase基因超表达对烟草生长的影响

doi:10.12190/j.issn.2096-1197.2021.04.10

北方农业学报 ›› 2021, Vol. 49 ›› Issue (4): 62-69.doi: 10.12190/j.issn.2096-1197.2021.04.10

• 分子生物学·生理生化 • 上一篇下一篇

SBPase基因超表达对烟草生长的影响

韩平安^1,2, 孙瑞芬^1,2, 唐宽刚^1,2, 常悦^1,2, 梁亚晖^1,2, 聂利珍^1,2, 吴新荣^1,2, 李晓东^1,2

1.内蒙古自治区农牧业科学院,内蒙古呼和浩特 010031;
2.内蒙古自治区甜菜品种遗传改良与种质创制重点实验室,内蒙古呼和浩特 010031

收稿日期:2021-07-13 出版日期:2021-08-20 发布日期:2021-10-28
通讯作者: 吴新荣（1967—）,男,研究员,博士,主要从事作物生物技术的研究工作。李晓东（1977—）,男,研究员,博士,主要从事作物生物技术的研究工作。
作者简介:韩平安（1987—）,女,助理研究员,博士,主要从事作物生物技术的研究工作。
基金资助:
内蒙古自治区农牧业创新基金项目（2018CXJJN07）; 内蒙古自治区科技重大专项项目（ZDZX2018015）; 内蒙古自治区科技计划项目（2021GG0026）; 内蒙古自治区“草原英才”工程高层次人才创新团队项目; 国家糖料产业技术体系项目（CARS-170104）

Effects of SBPase gene overexpression on tobacco growth

HAN Ping′an^1,2, SUN Ruifen^1,2, TANG Kuangang^1,2, CHANG Yue^1,2, LIANG Yahui^1,2, NIE Lizhen^1,2, WU Xinrong^1,2, LI Xiaodong^1,2

1. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;
2. Inner Mongolia Key Laboratory of Sugarbeet Genetics and Germplasm Enhancement,Hohhot 010031,China

Received:2021-07-13 Online:2021-08-20 Published:2021-10-28

摘要/Abstract

摘要： 【目的】探究SBPase基因超表达对烟草农艺性状与光合特性的影响。【方法】采用含SBPase基因的表达载体转化烟草叶片,获得转基因烟草株系L1、L2、L3,以野生型烟草WT为对照,利用微滴式数字PCR技术筛选低拷贝转化植株,并测定转基因烟草农艺性状与光合特性。【结果】3个转基因烟草株系植株的茎围、叶长均显著高于野生型烟草（P<0.05）,茎围分别增加了52.47%、21.34%和49.17%,叶长分别增加了30.77%、25.96%和39.10%;叶宽均极显著高于野生型烟草（P<0.01）,分别增加了55.48%、53.82%、65.39%;叶片数和株高均高于野生型烟草,但未达到显著差异水平（P>0.05）。3个转基因烟草株系的净光合速率显著高于野生型烟草（P<0.05）,分别提高了88.2%、43.6%和27.1%;蒸腾速率、气孔导度均略高于野生型烟草,但未达到显著差异水平（P>0.05）;胞间CO₂浓度与野生型烟草达到显著差异水平（P<0.05）,分别提高了13.0%、4.4%和4.3%。【结论】SBPase基因超表达使烟草光合性能大幅度提高,能够积累更多的光合作用产物,从而促进自身生长。

关键词: 烟草, SBPase基因, 超表达, 农艺性状, 光合特性, 微滴式数字PCR

Abstract: 【Objective】To investigate the effects of SBPase gene overexpression on agronomic traits and photosynthetic characteristics of tobacco.【Methods】Tobacco leaves were transformed with expression vectors containing SBPase gene to obtain transgenic tobacco lines L1,L2,and L3. Wild-type tobacco WT was used as control,and low-copy transgenic plants were screened by microdrop digital PCR,and agronomic traits and photosynthetic characteristics of transgenic tobacco were measured.【Results】It was found that the stem circumference and leaf length of three transgenic tobacco lines was significantly（P<0.05） higher than that of wild-type,the stem circumference increased by 52.47%,21.34%,and 49.17%,respectively;leaf length increased by 30.77%,25.96%,and 39.10%,respectively. The leaf width was extremely significantly higher（P<0.01）than that of wild-type tobacco by 55.48%,53.82%,and 65.39%,respectively. The number of leaves and plant height of transgenic tobacco were higher than that of wild-type tobacco,but the difference were not significant（P>0.05）. The net photosynthetic rates of three transgenic tobacco lines were significantly（P<0.05）higher than those of wild-type tobacco by 88.2%,43.6%,and 27.1%,respectively. The transpiration rate and stomatal conductance were slightly higher than those of wild-type tobacco,but the differences were not significant（P>0.05）. Intercellular CO₂ concentration was significantly（P<0.05）higher than that of wild-type tobacco,which was 13.0%,4.4%,and 4.3% higher,respectively.【Conclusion】The overexpression of SBPase gene greatly improved the photosynthetic performance of tobacco,which could accumulate more photosynthetic products and promote its own growth.

Key words: Tobacco, SBPase gene, Overexpression, Agronomic traits, Photosynthetic characteristics, Microdrop digital PCR

中图分类号:

S572

韩平安, 孙瑞芬, 唐宽刚, 常悦, 梁亚晖, 聂利珍, 吴新荣, 李晓东. SBPase基因超表达对烟草生长的影响[J]. 北方农业学报, 2021, 49(4): 62-69.

HAN Ping′an, SUN Ruifen, TANG Kuangang, CHANG Yue, LIANG Yahui, NIE Lizhen, WU Xinrong, LI Xiaodong. Effects of SBPase gene overexpression on tobacco growth[J]. Journal of Northern Agriculture, 2021, 49(4): 62-69.

参考文献

[1] ZHU X G,LONG S P,ORT D R.Improving photosynthetic efficiency for greater yield[J].Annual Review of Plant Biology,2010,61(1):235-261.
[2] ORR D J,PEREIRA A M,DA FONSECA PEREIRA P,et al.Engineering photosynthesis:progress and perspectives[J].F1000Research,2017,6:1891.
[3] DE PORCELLINIS A J,NØRGAARD H,BREY L M F,et al.Overexpression of bifunctional fructose-1,6-bisphosphatase/sedoheptulose-1,7-bisphosphatase leads to enhanced photosynthesis and global reprogramming of carbon metabolism in Synechococcus sp. PCC7002[J].Metabolic Engineering,2018,47:170-183.
[4] JANASCH M,ASPLUND-SAMUELSSON J,STEUER R,et al.Kinetic modeling of the Calvin cycle identifies flux control and stable metabolomes in Synechocystis carbon fixation[J].Journal of Experimental Botany,2019,70(3):973-983.
[5] 王静华. 黄淮海大豆冀豆17和冀nf58的遗传基础分析及重要蛋白挖掘[D].石家庄:河北师范大学,2015.
[6] HARRISON E P,OLCER H,LLOYD J C,et al.Small decreases in SBPase cause a linear decline in the apparent RuBP regeneration rate,but do not affect Rubisco carboxylation capacity[J].Journal of Experimental Botany,2001,52(362):1779-1784.
[7] 王俊秀,周扬颜,韩潇,等.超表达杨树SBPase基因促进拟南芥光合作用及营养生长[J].北京林业大学学报,2018,40(3):26-33.
[8] DING F,WANG M L,ZHANG S X,et al.Changes in SBPase activity influence photosynthetic capacity,growth,and tolerance to chilling stress in transgenic tomato plants[J].Scientific Reports,2016,6:32741.
[9] LI C,LI N,HUANG R,et al.A single nucleotide substitution at the 3′-end of SBPase gene involved in Calvin cycle severely affects plant growth and grain yield in rice[J].BMC Pant Biology,2020,20(1):345.
[10] MASAHIRO T,MIKI N,YOSHIKO M,et al.Contribution of fructose-1,6-bisphosphatase and sedoheptulose-1,7-bisphosphatase to the photosynthetic rate and carbon flow in the Calvin cycle in transgenic plants[J].Plant & Cell Physiology,2006,47(3):380-390.
[11] 冯玲玲,陈琼,李玉梦,等.蓝藻FBP/SBPase的制备及其与配体相互作用的分析——科研转化的化学生物学综合实验设计[J].大学化学,2021,36(6):116-123.
[12] LEFEBVRE S,LAWSON T,ZAKHLENIUK O V, et al.Increased sedoheptulose-1,7-bisphosphatase activity in transgenic tobacco plants stimulates photosynthesis and growth from an early stage in development[J].Plant Physiology,2005,138(1):451-460.
[13] 习雨琳. 一些单、双子叶植物RBCS基因启动子的克隆及其表达特性分析[D].北京:中国农业科学院,2012.
[14] 王盛,谢芝勋,谢丽基,等.转基因烟草中外源基因实时荧光定量PCR检测方法的建立[J].南方农业学报,2015,46(5):745-749.
[15] 国家烟草专卖局.烟草农艺性状调查测量方法:YC/T 142—2010[S].北京:中国标准出版社,2010.
[16] RAINES C A,PAUL M J.Products of leaf primary carbon metabolism modulate the developmental programme determining plant morphology[J].Journal of Experimental Botany,2006,57(9):1857-1862.
[17] VON CAEMMERER S,EVANS J R.Enhancing C3 photosynthesis[J].Plant Physiology,2010,154(2):589-592.
[18] RUAN C J,SHAO H B,DA SILVA J A T.A critical review on the improvement of photosynthetic carbon assimilation in C3 plants using genetic engineering[J].Critical Reviews in Biotechnology,2012,32(1):1-21.
[19] HAMMEL A,SOMMER F,ZIMMER D,et al.Overexpress-ion of sedoheptulose-1,7-bisphosphatase enhances pho-tosynthesis in Chlamydomonas reinhardtii and has no effect on the abundance of other Calvin-benson cycle enzymes[J].Frontiers in Plant Science,2020,11:868.
[20] DRIEVER S M,SIMKIN A J,ALOTAIBI S,et al.Increased SBPase activity improves photosynthesis and grain yield in wheat grown in greenhouse conditions[J].Philosophical Transactions:Biological Sciences,2017,372(1730):1-10.
[21] 袁传忠,韩雪娟,李轶群,等.桑树景天庚酮糖-1,7-二磷酸酶基因的遗传转化及生物学功能分析[J].蚕业科学,2013,39(3):413-419.
[22] LAWSON T,BRYANT B,LEFEBVRE S,et al.Decreased SBPase activity alters growth and development in transgenic tobacco plants[J].Plant,Cell Environment,2006,29(1):48-58.
[23] STEPHANE L,TRACY L,OKSANA V Z,et al.Increased sedoheptulose-1,7-bisphosphatase activity in transgenic tobacco plants stimulates photosynthesis and growth from an early stage in development[J].Plant Physiology,2005,138(1):451-460.
[24] 姚新转,吕立堂,赵德刚.转拟南芥BAS1基因提高烟草光合特性[J].山地农业生物学报,2016,35(3):30-34.
[25] 范繁,刘建国,白国辉,等.转基因工程植物启动子的研究进展[J].贵州农业科学,2015,43(4):25-30.

SBPase基因超表达对烟草生长的影响

Effects of SBPase gene overexpression on tobacco growth

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