北方农业学报 ›› 2023, Vol. 51 ›› Issue (6): 18-27.doi: 10.12190/j.issn.2096-1197.2023.06.03

• 土壤肥料·生理生化 • 上一篇    下一篇

PEG模拟干旱胁迫对不同糜子品种萌发的影响

胡玉璐1,2,3, 冯慧4, 任江陵1,2,3, 刘宇涵1,2,3, 刘思辰1,2,3, 乔治军1,2,3, 曹晓宁1,2,3   

  1. 1.山西农业大学 农业基因资源研究中心,山西 太原 030031;
    2.山西农业大学 农学院,山西 太谷 030801;
    3.农业农村部黄土高原作物基因资源与种质创制重点实验室,山西 太原 030031;
    4.呼伦贝尔市农牧技术推广中心,内蒙古 呼伦贝尔 021008
  • 收稿日期:2023-09-11 出版日期:2023-12-20 发布日期:2024-02-19
  • 通讯作者: 曹晓宁(1985—),男,副研究员,博士,硕士生导师,主要从事谷子糜子种质资源创新利用及抗逆生理的研究工作。乔治军(1964—),男,研究员,学士,博士生导师,主要从事谷子糜子种质资源创新利用及抗逆生理的研究工作。
  • 作者简介:胡玉璐(1997—),女,硕士研究生,研究方向为旱作栽培与作物生理。
  • 基金资助:
    谷子高粱产业技术体系谷子糜子生理岗位(CARS-06-14.5-A16); 山西省现代农业产业技术体系建设专项资金项目(2023CYJSTX03-23); 中央引导地方科技发展资金项目(YDZJSX2022A044)

Effects of PEG simulated drought stress on the germination of different Panicum miliaceum L. varieties

HU Yulu1,2,3, FENG Hui4, REN Jiangling1,2,3, LIU Yuhan1,2,3, LIU Sichen1,2,3, QIAO Zhijun1,2,3, CAO Xiaoning1,2,3   

  1. 1. Center for Agricultural Genetic Resources Research,Shanxi Agricultural University,Taiyuan 030031,China;
    2. College of Agriculture,Shanxi Agricultural University,Taigu 030801,China;
    3. Key Laboratory of Loess Plateau Crop Gene Resources and Germplasm Enhancement,Ministry of Agriculture and Rural Affairs,Taiyuan 030031,China;
    4. Hulun Buir Agricultural and Animal Husbandry Technology Promotion Center,Hulun Buir 021008,China
  • Received:2023-09-11 Online:2023-12-20 Published:2024-02-19

摘要: 【目的】探究干旱胁迫对不同糜子品种的萌发影响,明确糜子萌发期的抗旱评价指标及不同品种的抗旱能力。【方法】以育成审(认)定且糜子主产区种植面积较大的20个糜子品种为试验材料,利用20%的PEG-6000溶液模拟干旱胁迫,测定发芽势、发芽率、胚芽长、胚芽鞘长、侧根数、胚根长、萌发指数和发芽指数8个萌发性状,用相关性分析和隶属函数分析进行糜子萌发期抗旱性评价。【结果】干旱胁迫下,20个糜子品种发芽势、发芽率、胚芽长、胚芽鞘长、侧根数、胚根长、萌发指数和发芽指数均受到抑制,其中,陇糜15号发芽势、发芽率、萌发指数和发芽指数受到的抑制最强,降低率分别为59.05%、43.05%、68.68%、65.99%;陇糜7号受到抑制较轻,降低率分别为20.41%、11.49%、40.51%、36.78%。隶属函数值综合评价结果表明,陇糜7号抗旱能力较强,为0.868,陇糜15号抗旱能力较弱,为0.093。相关性分析表明,相对萌发指数、相对发芽势、相对胚芽长、相对胚芽鞘长、相对胚根长、相对侧根数与隶属函数综合评价值(D值)显著相关。【结论】筛选出了抗旱能力较强的品种陇糜7号和抗旱能力较弱品种陇糜15号;相对萌发指数、相对发芽势、相对胚芽长、相对胚芽鞘长、相对胚根长、相对侧根数可作为糜子萌发期抗旱评价的参考指标。

关键词: 糜子, 品种筛选, 抗旱评价, 干旱胁迫, 萌发期

Abstract: 【Objective】Explore the effect of drought stress on the germination of different Panicum miliaceum L. varieties,to clarify the drought resistance evaluation index of Panicum miliaceum L. during germination stage and the drought resistance ability of different varieties.【Methods】Twenty registered Panicum miliaceum L. varieties with large planting areas in the main producing areas were used as experimental materials. 20% PEG-6000 solution was used to simulate drought stress. Eight germination traits including germination potential,germination rate,embryo length,coleoptile length,lateral root number,radicle length,germination index and sprout index were measured. The drought resistance of Panicum miliaceum L. at germination stage was evaluated by correlation analysis and membership function analysis.【Results】Under drought stress,the germination potential,germination rate,embryo length,coleoptile length,lateral root number,radicle length,germination index and sprout index of the 20 Panicum miliaceum L. varieties were all inhibited. Among them,the germination potential,germination rate,germination index and sprout index of Longmei No. 15 were inhibited most,with reduction rates of 59.05%,43.05%,68.68%,and 65.99%,respectively. Longmei No. 7 were less inhibited,with reduction rates of 20.41%,11.49%,40.51%,and 36.78%,respectively. The membership function value comprehensive evaluation results showed that the drought resistance ability of Longmei No. 7 was stronger at 0.868,while Longmei No. 15 was weaker at 0.093. Correlation analysis showed that,relative germination index,relative germination potential,relative embryo length,relative coleoptile length,relative radicle length,and relative lateral root number were significantly correlated with the membership function comprehensive evaluation value(D value). 【Conclusion】Longmei No. 7 was screened as strong drought resistance variety while Longmei No. 15 was the weak drought resistance variety. Relative germination index,relative germination potential,relative embryo length,relative coleoptile length,relative radicle length and relative lateral root number can be used as reference indexes for drought resistance evaluation of Panicum miliaceum L. at germination stage.

Key words: Panicum miliaceum L., Variety screening, Drought resistance evaluation, Drought stress, Germination stage

中图分类号: 

  • S516