不同秸秆还田方式对土默川平原灌区土壤细菌群落的影响

doi:10.12190/j.issn.2096-1197.2024.04.05

北方农业学报 ›› 2024, Vol. 52 ›› Issue (4): 38-49.doi: 10.12190/j.issn.2096-1197.2024.04.05

不同秸秆还田方式对土默川平原灌区土壤细菌群落的影响

赵文山^1,2, 冯彪^1,2, 刘瑞枝^1,2, 梁红伟^2,3, 于晓芳^1,2, 高聚林^1,2, 青格尔^1,2

1.玉米秸秆原位还田微生物内蒙古自治区工程研究中心, 内蒙古呼和浩特 010019;
2.内蒙古自治区作物栽培与遗传改良重点实验室, 内蒙古呼和浩特 010019;
3.内蒙古自治区农牧业科学院, 内蒙古呼和浩特 010031

收稿日期:2024-03-05 出版日期:2024-08-20 发布日期:2024-11-22
通讯作者: 青格尔（1988—）,女,副教授,博士,硕士生导师,主要从事寒旱区作物栽培生理生态研究工作。
作者简介:赵文山（1998—）,男,硕士研究生,研究方向为寒旱区作物栽培生理生态研究。
基金资助:
国家重点研发计划项目（2023YFD2301801,2023YFD1501500-01）;国家自然科学基金项目（32260532）;内蒙古自治区直属高校基本科研业务费项目（BR22-11-07）;内蒙古自治区高等学校碳达峰碳中和研究专项（STZX202304）;内蒙古自治区科技计划项目（2023KYPT0023）;国家玉米产业技术体系（CARS-02-74）;内蒙古自治区研究生科研创新项目（S20231103Z）

Effects of different straw returning methods on soil bacterial communityin Tumochuan Plain irrigation area

ZHAO Wenshan^1,2, FENG Biao^1,2, LIU Ruizhi^1,2, LIANG Hongwei^2,3, YU Xiaofang^1,2, GAO Julin^1,2, Qinggeer^1,2

1. Inner Mongolia Autonomous Region Engineering Research Center for in situ Returning Microorganisms of Corn Straw, Hohhot 010019, China;
2. Key Laboratory of Crop Cultivation and Genetic Improvement in Inner Mongolia Autonomous Region, Hohhot 010019, China;
3. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China

Received:2024-03-05 Online:2024-08-20 Published:2024-11-22

摘要/Abstract

摘要： 【目的】探索不同秸秆还田方式对土默川平原灌区土壤细菌群落的影响。【方法】设置深松混耙秸秆还田（SSR）、条深旋秸秆还田（SCR）、深翻粉碎秸秆还田（DPR）和免耕秸秆覆盖还田（NTR）4个处理,以当地农户浅旋秸秆离田（CK）为对照,开展不同秸秆还田方式定位试验,采用16S rDNA扩增子高通量测序技术研究土壤微生物群落多样性,并分析其与土壤物理化学特性以及酶活性等关键环境因子间的相关性。【结果】不同秸秆还田方式显著影响土壤细菌多样性及群落结构（P<0.05）;NTR处理显著增加了芽单胞菌门和厚壁菌门丰度（P<0.05）,DPR处理中绿弯菌门丰度显著提高（P<0.05）、放线菌门丰度降低;各秸秆还田处理可显著改善土壤物理化学特性,提高土壤酶活性,其中SSR处理效果最显著。RDA分析发现,驱动土壤细菌群落组成的主要物理化学特性为碱解氮、速效钾、阳离子交换量、紧实度和容重,Arthrobacter、Gaiella、Solirubrobacter、Streptomyces易受土壤环境因子的影响。【结论】秸秆还田对土默川平原灌区土壤细菌群落均有不同程度的促进作用。

关键词: 秸秆还田, 高通量测序, 土壤细菌群落, 微生物群落结构

Abstract: 【Objective】To explore the effects of different straw returning methods on soil bacterial community in Tumochuan Plain irrigation area.【Methods】Four corn straw returning modes including subsoiling returning straw（SSR）,deep spin returning straw（SCR）,deep tillage returning straw（DPR）,no-tillage returning straw（NTR）,and farmer′s shallow rotation（CK）were the positioning tests. Soil bacterial community diversity was investigated by 16S rDNA amplicon high-throughput sequencing technology,and its correlation with soil physicochemical properties,enzyme activities were analyzed.【Results】There were significant effects in soil bacterial diversity and community composition among different straw returning treatments（P<0.05）,in which,NTR treatment significantly enhanced the abundance of Gemmatimonadetes and Firmicutes（P<0.05）,the abundance of Chloroflexi in DPR treatment was significantly increased（P<0.05）,and the abundance of Actinobacteria was decreased. That the straw returing treatments could significantly improve the soil physicochemical properties and increase soil enzyme activities,among which SSR treatment had the most significant improvement effect（P<0.05）. RDA analysis found that the main physicochemical properties that drive the composition of the soil bacterial community were alkaline hydrolysis nitrogen,available potassium,cation exchange capacity,compaction and bulk density,Arthrobacter,Gaiella,Solirorobacter,Streptomyces were susceptible to soil environmental factors.【Conclusion】The straw returning has a positive effect on the improvement of the soil biological community in the Tumochuan Plain irrigation area.

Key words: Straw returing, High-throughput sequencing, Soil bacterial community, Microbial community structure

中图分类号:

S154.3

赵文山, 冯彪, 刘瑞枝, 梁红伟, 于晓芳, 高聚林, 青格尔. 不同秸秆还田方式对土默川平原灌区土壤细菌群落的影响[J]. 北方农业学报, 2024, 52(4): 38-49.

ZHAO Wenshan, FENG Biao, LIU Ruizhi, LIANG Hongwei, YU Xiaofang, GAO Julin, Qinggeer. Effects of different straw returning methods on soil bacterial communityin Tumochuan Plain irrigation area[J]. Journal of Northern Agriculture, 2024, 52(4): 38-49.

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不同秸秆还田方式对土默川平原灌区土壤细菌群落的影响

Effects of different straw returning methods on soil bacterial communityin Tumochuan Plain irrigation area

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