北方农业学报 ›› 2024, Vol. 52 ›› Issue (5): 21-28.doi: 10.12190/j.issn.2096-1197.2024.05.03

• 分子生物学·生理生化 • 上一篇    下一篇

基于ISSR的8个羊肚菌菌株遗传多样性分析

于海宾1, 鲍红春2, 李小雷1, 任益芳1, 宗海龙1, 庞杰3, 于传宗3, 王燕春4, 张文光5   

  1. 1.内蒙古农业大学 农学院,内蒙古 呼和浩特 010019;
    2.内蒙古自治区农牧业技术推广中心,内蒙古 呼和浩特 010011;
    3.内蒙古自治区农牧业科学院,内蒙古 呼和浩特 010031;
    4.赤峰市农牧科学研究所,内蒙古 赤峰 024000;
    5.达拉特旗农牧业综合行政执法大队,内蒙古 达拉特旗 014300
  • 收稿日期:2024-05-29 出版日期:2024-10-20 发布日期:2025-01-14
  • 通讯作者: 鲍红春(1979—),女,研究员,博士,主要从事食用菌栽培与种质创新方面的研究工作。
  • 作者简介:于海宾(1998—),女,硕士,主要从事食用菌栽培与种质创新方面的工作。
  • 基金资助:
    内蒙古自治区科技计划项目(2020GG0223,2021GG0175,2022YFHH0110); 内蒙古自治区“揭榜挂帅”项目(2022JBGS0028)

Genetic diversity analysis of 8 Morchella esculents strains based on ISSR

YU Haibin1, BAO Hongchun2, LI Xiaolei1, REN Yifang1, ZONG Hailong1, PANG Jie3, YU Chuanzong3, WANG Yanchun4, ZHANG Wenguang5   

  1. 1. College of Agriculture,Inner Mongolia Agricultural University,Hohhot 010019,China;
    2. Inner Mongolia Agricultural and Animal Husbandry Technology Promotion Center,Hohhot 010011,China;
    3. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;
    4. Chifeng Institute of Agricultural and Animal Husbandry Sciences,Chifeng 024000;
    5. Dalad Banner Agriculture and Animal Husbandry Comprehensive Administrative Law Enforcement Brigade,Dalad Banner 014300,China
  • Received:2024-05-29 Online:2024-10-20 Published:2025-01-14

摘要: 【目的】明确羊肚菌种质资源间的亲缘关系和多样性,为选育适宜内蒙古西部地区栽培的新品种提供参考。【方法】以A3、赵二六、羊贵妃、金秋、DY008、A芦、001、内石林8个羊肚菌菌株为试验材料,利用ISSR分子标记技术对8个羊肚菌菌株进行聚类分析和遗传多样性比较。【结果】利用100条ISSR引物对8个羊肚菌菌株进行PCR检测,筛选出12条ISSR引物,扩增出DNA条带76条。其中,有效条带59条,多态性比率为75.83%。基因多样性指数(H)为0.206 6,Shannon′s信息指数(I)为0.330 0。供试菌株的遗传距离变幅最大的为1.072 6;在GS值约为0.38时,将菌株分为5个类群。【结论】供试羊肚菌菌株之间具有明显的遗传差异性。

关键词: 羊肚菌, ISSR, 遗传多样性, 聚类分析

Abstract: 【Objective】To clarify the phylogenetic relationship and diversity of Morchella esculents germplasm resources,and to provide reference for the selection and breeding of new varieties adapted to the cultivation in the western region of Inner Mongolia.【Methods】8 Morchella esculents strains(A3,Zhao′erliu,Yangguifei,Jinqiu,DY008,A Lu,001 and Neishilin)were used as experimental materials,ISSR molecular marker technology was used to perform cluster analysis and genetic diversity comparison on 8 strains of Morchella esculents.【Results】Eight Morchella esculents strains were detected by PCR using 100 ISSR primers,12 ISSR primers were selected,and 76 DNA bands were amplified. There were 59 effective bands,and the polymorphism ratio was 75.83%. The gene diversity index (H) was 0.206 6,and Shannon′s information index(I) was 0.330 0. The maximal variation of genetic distance was 1.072 6. When the GS value was about 0.38,the strains were divided into 5 groups.【Conclusion】There was significant genetic variability among the tested Morchella esculents strains.

Key words: Morchella esculents, ISSR, Genetic diversity, Cluster analysis

中图分类号: 

  • S646.7