北方农业学报 ›› 2024, Vol. 52 ›› Issue (5): 71-83.doi: 10.12190/j.issn.2096-1197.2024.05.08

• 分子生物学·生理生化 • 上一篇    下一篇

不同来源淫羊藿的质量评价

何洁1, 杨洁2,3, 王仕宝2,3, 丁伟1, 崔艳丽4, 张萌萌2,3, 黄东萍1, 张慧2,3, 何志鹏5, 黄文1   

  1. 1.汉中市食品药品监督检验检测中心,陕西 汉中 723000;
    2.汉中职业技术学院 药学院,陕西 汉中 723000;
    3.汉中职业技术学院 秦巴山区药(食)用植物研究所,陕西 汉中 723000;
    4.汉中职业技术学院 基础课教学部,陕西 汉中 723000;
    5.陕西国际商贸学院 医药学院,陕西 咸阳 712046
  • 收稿日期:2024-04-11 出版日期:2024-10-20 发布日期:2025-01-14
  • 通讯作者: 王仕宝(1982—),男,教授,硕士,主要从事药用植物资源开发利用方面的研究工作。
  • 作者简介:何 洁(1972—),女,副主任药师,学士,主要从事中药分析检测方面的研究工作。
  • 基金资助:
    2017年中医药公共卫生服务补助专项项目(财社〔2017〕66号); 2023年汉中职业技术学院科研课题(2023YJN20,2023YJN21); 2019年汉中青年科技创新团队(汉市科发〔2019〕26号); 2023年度汉中职业技术学院数学建模青年科研创新团队(汉职院发〔2023〕85号)

Quality evaluation of Epimedii Folium from different sources

HE Jie1, YANG Jie2,3, WANG Shibao2,3, DING Wei1, CUI Yanli4, ZHANG Mengmeng2,3, HUANG Dongping1, ZHANG Hui2,3, HE Zhipeng5, HUANG Wen1   

  1. 1. Hanzhong Center for Food and Drug Supervision,Inspection and Testing,Hanzhong 723000,China;
    2. Faculty of Pharmacy,Hanzhong Vocational and Technical College,Hanzhong 723000,China;
    3. Qinba Mountains Institute of Pharmaceutical(Edible) Botanty,Hanzhong Vocational and Technical College,Hanzhong 723000,China;
    4. Department of Basic Courses Teaching,Hanzhong Vocational and Technical College,Hanzhong 723000,China;
    5. Medical College,Shaanxi University of International Trade and Commerce,Xianyang 712046,China
  • Received:2024-04-11 Online:2024-10-20 Published:2025-01-14

摘要: 【目的】构建不同来源淫羊藿的质量评价方法。【方法】采用高效液相色谱法测定30批淫羊藿样品中朝藿定A、朝藿定B、朝藿定C和淫羊藿苷4种黄酮醇苷类成分的含量,通过紫外分光光度计测定淫羊藿中总黄酮含量,并测定醇溶性浸出物和总灰分。利用灰色关联度分析法结合SPSS 27.0和Simca 14.1软件进行主成分分析和聚类分析,对不同来源淫羊藿进行质量评价。【结果】30批淫羊藿样品的最优参考序列关联度、最差参考序列关联度和相对关联度分别为0.26~0.62、0.35~0.76、0.27~0.63;主成分分析结果表明,提取特征值大于1的3个主成分特征值分别为3.179、1.964和1.185,方差贡献率分别为39.733%、24.554%和14.818%,累计方差解释率为79.102%。在聚类分析中,选择平方欧氏距离为5时,其样品分为6类。【结论】灰色关联度分析法结合SPSS 27.0和Simca 14.1软件,可以对淫羊藿品质进行较为全面可靠的评价。

关键词: 淫羊藿, 灰色关联度分析法, 主成分分析, 聚类分析, 质量评价

Abstract: 【Objective】To constructed the quality evaluation methods of Epimedii Folium from different sources.【Methods】High performance liquid chromatography(HPLC) was used to determine the four flavonoids of epimedin A,epimedin B,epimedin C and icariin of the 30 batches of Epimedii Folium samples. The content of total flavonoids in the samples were determined by ultraviolet spectrophotometer(UV),and the alcohol soluble extract and total ash were also measured. Using grey relational analysis(GRA) combined with SPSS 27.0 and Simca 14.1 software for principal component analysis and cluster analysis,evaluate the quality of Epimedii Folium from different sources.【Results】The optimal reference sequence correlation,the worst reference sequence correlation and the relative correlation of the 30 batches of Epimedii Folium samples were 0.26-0.62,0.35-0.76 and 0.27-0.63,respectively. The results of principal component analysis show that the eigenvalues of the three principal components with eigenvalues greater than 1 were 3.179,1.964 and 1.185,respectively. The variance contribution rates were 39.733%,24.554% and 14.818%,respectively. The cumulative variance interpretation rate was 79.102%. In the cluster analysis,when the square Euclidean distance was 5,the samples were divided into 6 categories.【Conclusion】The GRA method combined with SPSS 27.0 and Simca 14.1 can evaluate the quality of Epimedii Folium comprehensively and reliably.

Key words: Epimedii Folium, Grey relational analysis, Principal component analysis, Cluster analysis, Quality evaluation

中图分类号: 

  • R282.71