北方农业学报 ›› 2012, Vol. ›› Issue (6): 24-24.

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冬葵愈伤组织诱导技术研究

黄凤兰[1] 赵永[1] 布和巴特[2] 李海峰[1] 卜志刚[1] 李跃[1] 曾健寒[1] 邹千稳[1] 陈玲玲[1]   

  1. [1]内蒙古民族大学生命科学学院,内蒙古通辽028000 [2]内蒙古民族大学蒙医药学院,内蒙古通辽028000
  • 出版日期:2012-12-20 发布日期:2012-12-20
  • 通讯作者: 黄凤兰
  • 作者简介:黄凤兰(1973-),女,山东菏泽人,教授,博士,主要从事植物基因工程与细胞工程研究工作。
  • 基金资助:
    内蒙古民族大学教育教学研究项目(No.2009053)

Study on the Callus Induction Technology of Malva verticillata L.

HUANG Feng-lan(College of Life Science,Inner Mongolia University for Nationalities,Tongliao 028000,China)   

  • Online:2012-12-20 Published:2012-12-20

摘要: 冬葵(Malva verticillata L.)是一种具有很高药用价值的蒙药,实验以冬葵叶片和茎段为外植体诱导愈伤组织,为冬葵的快繁和细胞培养奠定基础。实验结果如下:冬葵叶片和茎段最适消毒条件是0.1%升汞分别消毒50s、2.5min;叶片和茎段诱导愈伤组织阶段最适培养基相同,均为MS+蔗糖(30g/L)+琼脂(7g/L)+NAA(0.4mg/L)+6-BA(3mg/L)+抗坏血酸(600mg/L)。

Abstract: Malva verticillata L.is a valuable medicinal plant in Mongolian pharmacy.In this paper,we carried out callus induction experiments with leaves and stem sections from this plant as explants,and laid a solid foundation for future rapid propagation and cell culture.The optimized experiment protocols were as follows.The leaves and stems were sterilized in HgCl 2 for 50 s or 2.5 min,and then placed on the developed medium containing MS,30g/L sucrose,7g/L Agar,0.4mg/L NAA,3mg/L 6-BA and 200mg/ L vitamin C.

中图分类号: 

  • S567