紫苏不同器官活性成分分布及功能性分析研究进展

doi:10.12190/j.issn.2096-1197.2021.05.17

摘要/Abstract

摘要： 紫苏是我国常用的中药材,为药食同源的植物资源之一,其药理活性成分非常丰富,具有抑菌、抗炎抗氧化、抑制肿瘤细胞等功效。文章总结了紫苏[Perilla frutescens（L.）Britt]不同器官的活性成分,分别对紫苏叶和茎中萜类、芳香族类、脂肪族类、黄酮类、酚酸类、苯丙素类、甾体类、苷类、氨基酸类物质及紫苏籽中的脂肪酸和蛋白质主要成分进行介绍,并对各成分功能性、抑菌机理进行综述,以期为紫苏的规范化种植及多元化应用提供理论依据。

关键词: 紫苏, 活性成分, 功能性分析, 抑菌机理

Abstract: Perilla frutescens（L.）Britt is one of the commonly used Chinese medicinal in China. It is one of the pharmaceutical and food homology plant resources. It has rich pharmacologically active ingredients,and has antibacterial,anti-inflammatory,antioxidant,and anti-tumor properties. This article summarized the active ingredients of different organs of Perilla frutescens（L.）Britt,reviewed terpenoids,aromatics,aliphatics,flavonoids,phenolic acids,phenylpropanoids,steroids,glycosides,and amion acids in leaves and stem,as well as main components of fatty acids and proteins in seeds. The functional and antibacterial mechanisms of each ingredient were also studied in order to provide a theoretical basis for the standardized planting and diversified application of Perilla frutescens（L.）Britt.

Key words: Perilla frutescens（L.）Britt, Active ingredient, Functional analysis, Antibacterial mechanism

中图分类号:

S636.9

古建兰, 任久强, 韦雪娇, 胡华林, 周丹. 紫苏不同器官活性成分分布及功能性分析研究进展[J]. 北方农业学报, 2021, 49(5): 118-126.

GU Jianlan, REN Jiuqiang, WEI Xuejiao, HU Hualin, ZHOU Dan. Research progress on the distribution and functional analysis of active components in Perilla frutescens（L.）Britt organs[J]. Journal of Northern Agriculture, 2021, 49(5): 118-126.

参考文献

[1] 刘长江,孔昭宸,朗树德.大地湾遗址农业植物遗存与人类生存的环境探讨[J].中原文物,2004(4):26-30.
[2] AHMED H M.Ethnomedicinal,phytochemical and pharmacological investigations of Perilla frutescens(L.)britt[J].Molecules,2018,24(1):102.
[3] 董玲婉,周丽娜.紫苏药理作用研究进展[J].中国药业,2008(1):61-62.
[4] 李玉邯,陈宇飞,杨柳,等.紫苏提取物对猪肉脯的抗氧化性能和抑菌效果的影响[J].中国调味品,2016,41(10):38-40.
[5] YANG H,SAN W,MA P,et al.Multiple components rapidly screened from perilla leaves attenuate asthma airway inflammation by synergistic targeting on syk[J].Journal of Inflammation Research,2020,13:897-911.
[6] WANG Z,TU Z,XIE X,et al.Perilla frutescens leaf extract and fractions:polyphenol composition,antioxidant,enzymes(α-glucosidase,acetylcholinesterase,and tyrosinase) inh-ibitory,anticancer,and antidiabetic activities[J].Foods,2021,10(2):315-334.
[7] 易家宁,王康才,张琪绮,等.干旱胁迫对紫苏生长及品质的影响[J].核农学报,2020,34(6):1320-1326.
[8] SUH D H,KIMY X,JUNG E S,et al.Characterization of metabolic changes underlow mineral supply(N,K,or Mg)and supplemental LED lighting(red,blue,or red-blue combination)in Perilla frutescens using a metabolomics approach[J].Molecules,2020,25(20):4714-4729.
[9] WU L,DENG Z,CAO L,et al.Effect of plant density on yield and quality of perilla sprouts[J].Scientific Reports,2020,10(1):9937-9945.
[10] 刘娟,雷焱霖,唐友红,等.紫苏的化学成分与生物活性研究进展[J].时珍国医国药,2010,21(7):1768-1769.
[11] 魏长玲,郭宝林.紫苏叶挥发油的不同化学型及研究进展[J].中国中药杂志,2015,40(15):2937-2944.
[12] 何育佩,郝二伟,谢金玲,等.紫苏药理作用及其化学物质基础研究进展[J].中草药,2018,49(16):3957-3968.
[13] 刘浏. 紫苏叶的研究进展[J].中国医学创新,2012,9(6):162-164.
[14] SEO W H,BAEK H H.Characteristic aroma-active compounds of korean perilla(Perilla frutescens Britton)leaf[J].Journal of Agricultural and Food Chemistry,2009,57(24):11537-11542.
[15] FUJITA T,FUNAYOSHI A,NAKAYAMA M.A phenyl-propanoid glucoside from Perilla frutescens[J].Phytochemistry,1994,37(2):543-546.
[16] 刘建辉,陈兰贵.紫苏油中主要化学成分的分离和鉴定[J].香料香精化妆品,1998(1):19-20.
[17] TTADA M,MATSUMOTO R,CHIBA K.An oxidative metabolite of perillaldehyde from Perilla frutescens[J].Phytochemistry,1996,43(4):803-804.
[18] 惠永正. 中药天然产物大全[M].上海:上海科学技术出版社,2010.
[19] 严德鹏,尹笃林,李谦和.催化异构桃金娘烯醛制备紫苏醛[J].零陵学院学报,2003(2):41-42.
[20] 陈永康,赵志刚,孙丽娟.液相色谱-飞行时间质谱法快速鉴定紫苏叶中的化学成分[J].医药导报,2013,32(3):371-374.
[21] 林梦南,苏平.响应面法优化紫苏挥发油的水蒸气提取工艺及其成分研究[J].中国食品学报,2012,12(3):52-60.
[22] BANNO N,AKIHISA T,TOKUDA H,et al.Triterpene acids from the leaves of Perilla frutescens and their anti-inflammatory and antitumor-promoting effects[J].Journal of the Agricultural Chemical Society of Japan,2004,68(1):85-90.
[23] 雷殷. 中药紫苏叶挥发油的提取与气相色谱分析[D].长春:吉林大学,2006.
[24] 冯劼,王薇,余陈欢.紫苏叶挥发油化学成分分析及其抗炎机制研究[J].海峡药学,2011,23(5):45-48.
[25] 唐英,陈欣,沈平孃.紫苏叶中挥发油类成分的指纹图谱研究[J].上海中医药杂志,2013,47(9):82-86.
[26] 黄亮辉. 紫苏化学成分药材质量分析研究[D].西安:西北大学,2011.
[27] 喻世涛,熊国玺,程华,等.不同提取方法对紫苏叶挥发性成分的影响[J].南方农业学报,2014,45(1):108-111.
[28] 金石诚,刘忠伟,沈奇,等.贵州紫苏叶中氨基酸及游离氨基酸含量分析[J].山地农业生物学报,2016,35(4):13-17.
[29] 孟青,冯毅凡,梁汉明,等.紫苏挥发油GC/MS分析[J].广东药学院学报,2004(6):590-591.
[30] 郑梅琴,魏燕霞,林瑞余.不同紫苏挥发油化学成分分析[J].湖北农业科学,2018,57(24):143-146.
[31] 刘阳,王驰,陈伟梅,等.紫苏籽与紫苏茎叶挥发油的提取及其成分分析[J].中国医院药学杂志,2014,34(22):1897-1902.
[32] 任淑清,孙长海,方洪壮,等.紫苏叶挥发油GC-MS与直观推导式演进特征投影法分析[J].中国药房,2008(12):914-916.
[33] NAKAZAWA T,YASUDA T,UEDA J,et al.Antidepressant-like effects of apigenin and 2,4,5-trimethoxycinnamic acid from Perilla frutescens in the forced swimming test[J].Biological & Pharmaceutical Bulletin,2003,26(4):474-480.
[34] 霍立娜,王威,刘洋,等.紫苏叶化学成分研究[J].中草药,2016,47(1):26-31.
[35] GU L,WU T,WANG Z.TLC bioautography-guided isolation of antioxidants from fruit of Perilla frutescens var. acuta[J].Food Science & Technology,2009,42(1):131-136.
[36] 代沙,吴卫,李钰.HPLC法测定不同品系紫苏酚类物质的含量[J].核农学报,2014,28(1):108-115.
[37] 代沙. 紫苏叶抗氧化物质提取、含量测定及抗氧化活性研究[D].雅安:四川农业大学,2013.
[38] 王芸,张玥莉.不同产地紫苏梗中总黄酮含量比较及其提取工艺的考察[J].上海医药,2018,39(13):76-79.
[39] 亢倩丽,李壮壮,范珊珊,等.基于UPLC-Q-Exactive-Orbitrap-MS的紫苏叶与紫苏梗化学成分分析[J].中国实验方剂学杂志,2020,26(13):156-162.
[40] 石玮婷,李荣,姜子涛.紫苏籽蛋白的提取及纯化研究[J].中国食品添加剂,2013(4):68-74.
[41] 蔡宁晨. 紫苏叶花色苷的提取分析及其功能的研究[D].杭州:浙江大学,2013.
[42] 胡晓丹,孙爱东,王彩霞,等.超声波辅助提取紫苏叶中花色素苷的工艺研究[J].食品工业科技,2008,29(6):183-185.
[43] 唐英,陈欣,沈平孃.紫苏叶中挥发油类成分的指纹图谱研究[J].上海中医药杂志,2013,47(9):82-86.
[44] NAKAJIMA A,YAMAMOTO Y,YOSHINAKA N,et al.A new flavanone and other flavonoids from green perilla leaf extract inhibit nitric oxide production in interleukin 1β-treated hepatocytes[J].Journal of the Agricultural Chemical Society of Japan,2015,79(1):138-146.
[45] 田清清,张雪兰,张志鹏,等.不同直径紫苏梗含量及特征图谱比较研究[J].广东药科大学学报,2020,36(3):345-351.
[46] 杨慧,马培,林明宝,等.紫苏叶化学成分、抗炎作用及其作用机制研究进展[J].中国药理学与毒理学杂志,2017,31(3):279-286.
[47] 孙也评. 紫苏叶提取物及其有效成分的抗菌活性研究[D].延吉:延边大学,2014.
[48] HE Y K,YAO Y Y,CHANG Y N.Characterization of anthocyanins in Perilla frutescens var. acuta extract by advanced UPLC-ESI-IT-TOF-MSⁿ method and their anticancer eioactivity[J].Molecules,2015,20(3):9155-9169.
[49] 郭力. 中药化学[M].北京:中国医药科技出版社,2018.
[50] RYU J H,SON H J,LEE S H,et al.Two neolignans from Perilla frutescens and their inhibition of nitric oxide synthase and tumor necrosis factor-α expression in murine macrophage cell line RAW 264.7[J].Cheminform,2002,12(4):649-651.
[51] FUJITA T,TERATO K,NAKAYAMA M.Two jasmonoid glucosides and a phenylvaleric acid glucoside from Perilla frutescens[J].Bioscience Biotechnology and Biochemistry,1996,60(4):732-735.
[52] ARITOMI M,KUMORI T,KAWASAKI T.Cyanogenic glycosides in leaves of Perilla frutescens var. acuta[J].Phytochemistry,1985,24(10):2438-2439.
[53] 沈奇,徐静,商志伟,等.紫苏梗中主要营养及药用成分评价[J].中国现代中药,2019,21(7):920-924.
[54] 温春秀,刘灵娣,胡彦,等.紫苏属植物药用成分含量比较[J].河北农业科学,2012,16(9):64-67.
[55] 张琛武,郭佳琪,郭宝林.紫苏中酚酸类成分研究进展[J].中国现代中药,2017,19(11):1651-1658.
[56] 王恒,高婷婷,谭勇,等.紫苏叶中氨基酸及微量元素含量的测定[J].安徽农业科学,2013,41(1):88-89.
[57] 顾文娟,朱陈珏,张建梅.紫苏的药理作用研究进展[J].黑龙江畜牧兽医,2006(8):26-28.
[58] 王鑫,张麟,徐龙鑫,等.紫苏的营养成分及在畜牧生产中的应用研究进展[J].贵州畜牧兽医,2019,43(4):4-6.
[59] FUKUSHIMA A,NAKAMURA M,SUZUKI H,et al.High-throughput sequencing and de novo assembly of red and green forms of the Perilla frutescens var. crispa transcriptome[J].PLoS One,2015,10(6):154-172.
[60] 许春芳,董喆,郑明明,等.不同产地的紫苏籽油活性成分检测与主成分分析[J].中国油料作物学报,2019,41(2):275-282.
[61] 胡彦,丁友芳,温春秀,等.紫苏属植物种子含油率及其脂肪酸组成[J].食品科学,2010,31(14):165-169.
[62] 沈奇,王仙萍,杨森,等.紫苏籽主要营养成分含量分析[J].西南农业学报,2019,32(8):1904-1909.
[63] 韩亚男,潘士钢,李海英,等.不同产地紫苏籽含油率及α-亚麻酸含量比较[J].食品安全导刊,2020(18):102.
[64] 刘宁,赵佳,武选民,等.紫苏籽中不同蛋白组分的功能性质研究[J].中国油脂,2019,44(6):45-49.
[65] 徐冰冰,王瑛瑶,栾霞,等.水酶法提取紫苏籽油脂和蛋白质的工艺条件[J].食品科学,2012,33(22):127-132.
[66] 魏雯,高婷婷,谭勇,等.紫苏不同部位的体外抑菌作用[J].医药导报,2014,33(2):149-151.
[67] 况弯弯. 紫苏挥发油微囊的制备与表征、抑菌活性研究[D].南昌:江西中医药大学,2020.
[68] 徐健,王珊,宋占俞,等.紫苏精油的抑菌活性及其精华滋养乳的制备和质量检测[J].时珍国医国药,2020,31(5):1119-1122.
[69] 袁康,胡振阳,卢臣,等.紫苏精油对两种霉菌的抑菌活性效果研究[J].粮食科技与经济,2019,44(11):85-89.
[70] 许倩,牛希跃,张冰冰,等.肉桂、紫苏精油对青霉和黑曲霉抑菌特性研究[J].食品研究与开发,2014,35(17):5-8.
[71] 魏磊,李晓,王学方,等.紫苏叶总三萜超声提取工艺优化及对10种常见致病菌的抑菌作用研究[J].中国药房,2018,29(16):2193-2197.
[72] 黄红雨,赵虎,金晓艳.紫苏叶花青素提取工艺及其活性研究[J].食品研究与开发,2018,39(14):50-55.
[73] 星萍,陈晓平,邢钰彬,等.紫苏迷迭香酸对D-半乳糖衰老模型小鼠的抗氧化作用[J].食品科技,2021,46(4):222-227.
[74] 田海娟,高琼,李一鸣,等.混菌发酵紫苏粕总黄酮提取优化及抗氧化性的研究[J].粮食与油脂,2020,33(12):106-110.
[75] 茹巧美,任国平,胡琼.聚乙二醇提取紫苏叶花色苷工艺优化及抗氧化活性[J].食品科技,2020,45(10):220-228.
[76] 李会珍,谭永兰,夏瑶瑶,等.紫苏籽壳原花青素纯化及抗氧化性研究[J].中国农学通报,2019,35(14):138-143.
[77] 赖亚辉,王再彬,张鑫,等.吉林地产紫苏油脂肪酸测定及其抗氧化作用的研究[J].食品安全导刊,2018(15):57-58.
[78] 李晚谊. 云南茶叶、甜菜和紫苏抗过敏功能的研究[D].重庆:西南农业大学,2001.
[79] 韦保耀,黄丽,滕建文,等.紫苏叶抗过敏作用的评价[J].食品科技,2006(8):284-286.
[80] 黄丽,韦保耀,滕建文.紫苏叶抗过敏有效成分的研究[J].食品科技,2005(5):90-93.
[81] 张世林. 紫苏抗过敏成分的研究与开发[D].太原:中北大学,2020.
[82] SANBONGI C,TAKANO H,OSAKABE N,et al.Rosmarinic acid in perilla extract inhibits allergic inflammation induced by mite allergen,in a mouse model[J].Clinical & Experimental Allergy,2010,34(6):971-977.
[83] 吴健,陶钦,葛超,等.紫苏醇芳基钌配合物的合成及抗肿瘤性能[J].无机化学学报,2020,36(7):1223-1232.
[84] 贺东亮. 紫苏多肽分离纯化及其抗肿瘤活性研究[D].太原:中北大学,2019.
[85] 王晓辉. 紫苏化学成分和抗肿瘤的研究[D].长春:吉林大学,2016.
[86] KIM Y R,NAM B,HAN A R,et al.Isoegomaketone from Perilla frutescens(L.)Britt stimulates MAPK/ERK pathway in human keratinocyte to promote skin wound healing[J].Evidence-Based Complementary and Alternative Medicine,2021,2021:1-8.
[87] LEE H,PARK E.Perilla frutescens extracts enhance DNA repair response in UVB damaged HaCaT cells[J].Nutrients,2021,13(4):1263-1279.
[88] BAE S J,KIM J E,CHOI H J,et al.α-Linolenic acid-enriched cold-pressed perilla oil suppress high-fat diet-induced hepatic steatosis through amelioration of the ER stress-mediated autophagy[J].Molecules,2020,25(11):2662-2679.
[89] LEE H,SUNG J,KIM Y H,et al.Protective effects of unsaponifiable matter from perilla seed meal on UVB-induced damages and the underlying mechanisms in human skin fibroblasts[J].Antioxidants(Basel),2019,8(12):644-656.
[90] HASHIMOTO M,MATSUZAKI K,KATO S,et al.Twelve-month studies on perilla oil intake in Japanese adults ossible supplement for mental health[J].Foods,2020,9(4):530-545.
[91] HASHIMOTO M,TANABE Y,HOSSAIN S,et al.Intake of alpha-linolenic acid-rich Perilla frutescens leaf powder decreases home blood pressure and serum oxidized low-density lipoprotein in Japanese adults[J].Molecules,2020,25(9):2099-2114.
[92] 冯林慧,李迎秋.紫苏的活性成分及其应用研究[J].中国调味品,2017,42(9):177-180.
[93] 袁康,胡振阳,陈可欣,等.紫苏精油抑制灰绿曲霉的活性与机理[J].食品科学,2020,41(23):63-69.
[94] 毛跟年,胡家欢,刘艺秀.野艾蒿提取物对金黄色葡萄球菌的抑菌机制研究[J].食品科技,2019,44(5):242-247.
[95] 李娜. 紫苏精油提取及其防腐复合材料的制备和性能研究[D].太原:中北大学,2018.
[96] 张嫚. 紫苏醛对甘薯采后黑斑病的防治及其病原真菌的作用机制[D].徐州:江苏师范大学,2018.