嫩江市大豆田反枝苋和藜对氟磺胺草醚的抗性

doi:10.12190/j.issn.2096-1197.2025.03.07

摘要/Abstract

摘要： 【目的】明确黑龙江省嫩江市大豆田阔叶杂草反枝苋和藜对氟磺胺草醚的抗性水平及分布情况。【方法】在嫩江镇、海江镇、前进镇等10个乡（镇）采集反枝苋和藜的种子,通过整株生物测定法明确反枝苋和藜对氟磺胺草醚的抗性水平,并通过靶标基因PPX2序列扩增及比对等方法明确反枝苋对氟磺胺草醚产生抗性的原因。【结果】在氟磺胺草醚不同剂量处理后,采集的30个反枝苋种群的GR₅₀为8.70~814.77 g a.i./hm²,抗性指数（RI）为17.8~93.6。处于低水平抗性的反枝苋种群有14个,主要采自联兴乡、伊拉哈镇、塔溪乡;处于中等水平抗性的反枝苋种群有10个,主要采自双山镇、长福镇、科洛镇;处于高水平抗性的反枝苋种群有2个,主要采自海江镇。经靶标基因检测后发现26个抗性反枝苋种群均在PPX2基因上发生了Arg-128Gly突变;选择1个抗性反枝苋种群R利用P450和GST抑制剂处理后发现,其RI明显降低,说明存在非靶标抗性。在氟磺胺草醚不同剂量处理后,采集的26个藜种群的GR₅₀为51.54 ~ 497.10 g a.i./hm²,RI为1.0~9.5。处于低水平抗性的藜种群有10个,中等水平抗性的藜种群有6个,尚未发现对氟磺胺草醚产生高水平抗性的藜种群;对氟磺胺草醚产生抗性的藜种群主要来自临江乡、长福镇、科洛镇。【结论】当前黑龙江省嫩江市反枝苋和藜已经对氟磺胺草醚产生了不同水平的抗性,其中反枝苋对氟磺胺草醚同时存在靶标与非靶标抗性。

关键词: 嫩江市, 大豆田, 反枝苋, 藜, 氟磺胺草醚, 抗性

Abstract: 【Objective】To determine the resistance levels and distribution of the broadleaf weeds Amaranthus retroflexus L. and Chenopodium album L. to fomesafen in soybean fields of Nenjiang City,Heilongjiang Province.【Methods】Seeds of A. retroflexus L. and C. album L. were collected from 10 towns,including Nenjiang Town,Haijiang Town,Qianjin Town etc. The resistance levels of weeds of A. retroflexus L. and C. album L. to fomesafen were determined using a whole-plant bioassay. The mechanisms underlying resistance in A. retroflexus L. were investigated through sequence amplification and alignment of the target gene PPX2 and other related methods.【Results】After treatment with different doses of fomesafen,the GR₅₀ values for 30 collected A. retroflexus L. populations ranged from 8.70 to 814.77 g a.i./hm²,with resistance indices（RI）of 17.8 to 93.6. Fourteen populations exhibited low-level resistance,primarily collected from Lianxing Township,Yilaha Town,and Taxi Township; ten populations showed moderate-level resistance,mainly from Shuangshan Town,Changfu Town,and Keluo Town; and two populations displayed high-level resistance,primarily from Haijiang Town. Target gene detection revealed that all 26 resistant A. retroflexus L. populations had an Arg-128Gly mutation in the PPX2 gene. A single resistant population of A. retroflexus L. R was selected and treated with P450 and GST inhibitors,resulting in a significant reduction of RI,indicating the presence of non-target-site resistance. After treatment with varying doses of fomesafen,the GR₅₀ values of 26 collected C. album L. populations ranged from 51.54 to 497.10 g a.i./hm²,with RI of 1.0 to 9.5. Ten populations exhibited low-level resistance,six showed moderate-level resistance,and no high-level resistant populations to fomesafen were identified. Resistant C. album L. populations to fomesafen were primarily collected from Linjiang Township,Changfu Town,and Keluo Town.【Conclusion】A. retroflexus L. and C. album L. in Nenjiang City,Heilongjiang Province,have developed different levels of resistance to fomesafen,with A. retroflexus L. exhibiting both target-site resistance and non-target-site resistance to fomesafen.

Key words: Nenjiang City, Soybean field, Amaranthus retroflexus L., Chenopodium album L., Fomesafen, Resistance

中图分类号:

S482.4

郭小桐, 郭玉莲, 王宇, 罗婵, 丛克强. 嫩江市大豆田反枝苋和藜对氟磺胺草醚的抗性[J]. 北方农业学报, 2025, 53(3): 60-75.

GUO Xiaotong, GUO Yulian, WANG Yu, LUO Chan, CONG Keqiang. Resistance of Amaranthus retroflexus L. and Chenopodium album L. to fomesafen in soybean fields of Nenjiang City[J]. Journal of Northern Agriculture, 2025, 53(3): 60-75.

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