北方农业学报 ›› 2022, Vol. 50 ›› Issue (5): 67-72.doi: 10.12190/j.issn.2096-1197.2022.05.10

• 植物保护 · 园艺 • 上一篇    下一篇

辣椒种质资源对疫霉病的抗病性评价及杀菌剂敏感性分析

孙平平1, 李正男1, 刘欣1, 张磊1, 王永2, 付崇毅2   

  1. 1.内蒙古农业大学 园艺与植物保护学院,内蒙古 呼和浩特 010011
    2.内蒙古自治区农牧业科学院 蔬菜花卉研究所,内蒙古 呼和浩特 010031
  • 收稿日期:2022-06-24 出版日期:2022-10-20 发布日期:2023-01-10
  • 通讯作者: 付崇毅
  • 作者简介:付崇毅(1986—),男,助理研究员,博士,主要从事园艺作物抗病性育种的研究工作。
    孙平平(1985—),女,讲师,博士,主要从事园艺作物病害病原生物学及综合防控的研究工作。
  • 基金资助:
    内蒙古自治区高等学校科学技术研究项目(NJZY21458);内蒙古自治区科技计划项目(2020GG0137);内蒙古自治区科技重大专项(2021SZD0040);“科技兴蒙”行动重点专项(211)

Resistance evaluation of pepper germplasm resources against pepper Phytophthora and fungicide sensitivity analysis on Phytophthora capsici

SUN Pingping1, LI Zhengnan1, LIU Xin1, ZHANG Lei1, WANG Yong2, FU Chongyi2   

  1. 1. College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010011,China
    2. Institute of Vegetable and Flower,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China
  • Received:2022-06-24 Online:2022-10-20 Published:2023-01-10
  • Contact: FU Chongyi

摘要:

【目的】评价辣椒种质资源对疫霉病的抗性水平,分析常见化学药剂对辣椒疫霉病的室内毒力,为该病的防控提供参考。【方法】采用灌根接种法接种,并对28份辣椒种质资源进行辣椒疫霉菌抗病性评价,然后以Phytophthora capsici S90为供试病原菌,利用菌丝生长速率法分析其对市场上16种常用杀菌剂的敏感性。【结果】不同辣椒种质资源的抗疫霉病能力差异显著,病情指数在0~100,其中,C164、C186、C167、C189、北星1号和北星6号的病情指数为0,表现为高抗,占资源总数的21.43%;中抗和高度感病的材料分别为1份和21份,分别占资源总数的3.57%和75.00%。16种杀菌剂中,32%吡唑醚菌酯·氟吡菌胺悬浮剂对P. capsici S90的抑制活性最高,其EC50值为6.935 0 mg/L;异菌脲、多菌灵、腈菌唑、乙蒜素和吡唑醚菌酯的EC50值为21.504 7~93.587 8 mg/L;其余杀菌剂的EC50值均大于100 mg/L,其中,氟菌·肟菌酯和氯溴异氰尿酸对P. capsici S90无抑制效果。【结论】供试辣椒种质资源中共有6份辣椒疫霉病的高抗品种;供试16份杀菌剂中,32%吡唑醚菌酯·氟吡菌胺悬浮剂对P. capsici S90的抑制活性最高,异菌脲、多菌灵、腈菌唑、乙蒜素和吡唑醚菌酯也具有一定的抑制活性,可用于辣椒疫霉病的防治。

关键词: 辣椒, 种质资源, 辣椒疫霉菌, 抗性评价, 药剂敏感性

Abstract:

【Objective】To assess the degree of Phytophthora resistance of pepper germplasm resources and examine the indoor antifungal activity of common chemical agents against Phytophthora capsici,in order to provide guidance for the prevention and control of Phytophthora.【Methods】The resistance of 28 pepper germplasm resources to Phytophthora capsici was evaluated by root inoculation. The sensitivity of Phytophthora capsici S90 to 16 common fungicides in the market was analyzed by mycelial growth rate method.【Results】The disease resistance of different pepper germplasm resources was significantly different,and the disease index was 0-100. Among them,the disease index of C164,C186,C167,C189,Beixing No.1 and Beixing No. 6 was 0,showing high resistance,accounting for 21.43% of the total resources. There were 1 medium resistant and 21 highly susceptible materials,accounting for 3.57% and 75.00% of the total resources,respectively. Among the 16 fungicides,the suspension of 32% pyraclostrobin·fluopicolide had the highest inhibitory activity of P. capsici S90,with EC50 value at 6.935 0 mg/L. The EC50 values of iprodione,carbendazim,myclobutanil,ethylicin and pyraclostrobin were 21.504 7 to 93.587 8 mg/L. The EC50 values of other fungicides were all greater than 100 mg/L,among which,fluopyramotri·floxystrobin and chlorobromoisocy anuric acid had no inhibitory effect on P. capsici S90.【Conclusion】In the tested pepper germplasm resources,there were six varieties with high Phytophthora capsici resistance. Among the 16 fungicides tested,the suspension of 32% pyraclostrobin·fluopicolide had the highest inhibitory effect on P. capsici S90. Iprodione,carbendazim,myclobutanil,ethylicin and pyraclostrobin also had some inhibitory activities that could be used as effective fungicides for controlling Phytophthora.

Key words: Pepper, Germplasm resources, Phytophthora capsici, Resistance evaluation, Fungicide susceptibility

中图分类号: 

  • S436