北方农业学报 ›› 2025, Vol. 53 ›› Issue (5): 36-48.doi: 10.12190/j.issn.2096-1197.2025.05.04

• 种质资源·植物保护 • 上一篇    下一篇

154份荞麦荧光特性评价及高光效种质筛选

胡昊驰1,2, 吴叶宁1, 谢锐1, 刘锦川1, 温蕊1, 王志超3, 王春勇4, 于传宗1, 金晓蕾1   

  1. 1.内蒙古自治区农牧业科学院,内蒙古 呼和浩特 010031;
    2.内蒙古农业大学 农学院,内蒙古 呼和浩特 010019;
    3.敖汉旗农牧局,内蒙古 敖汉旗 024300;
    4.唐山市农业科学研究院,河北 唐山 063001
  • 收稿日期:2025-07-09 出版日期:2025-10-20 发布日期:2026-01-20
  • 通讯作者: 金晓蕾(1988—),女,副研究员,博士,主要从事荞麦育种与栽培方面的研究工作。 于传宗(1977—),男,研究员,博士,硕士生导师,主要从事作物育种与栽培方面的研究工作。
  • 作者简介:胡昊驰(1996—),男,研究实习员,硕士,主要从事荞麦育种与栽培方面的研究工作。
  • 基金资助:
    国家重点研发计划项目(2023YFD1600700); 内蒙古农牧业科研保障经费项目(2023CXJJN05); 2022年度引进高层次人才科研支持(RZ2400001486)

Evaluation of fluorescence characteristics and screening of high light-use efficiency germplasm in 154 buckwheat varieties

HU Haochi1,2, WU Yening1, XIE Rui1, LIU Jinchuan1, WEN Rui1, WANG Zhichao3, WANG Chunyong4, YU Chuanzong1, JIN Xiaolei1   

  1. 1. Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences,Hohhot 010031,China;
    2. College of Agriculture,Inner Mongolia Agricultural University,Hohhot 010019,China;
    3. Aohan Banner Agriculture and Animal Husbandry Bureau,Aohan Banner 024300,China;
    4. Tangshan Academy of Agricultural Sciences,Tangshan 063001,China
  • Received:2025-07-09 Online:2025-10-20 Published:2026-01-20

摘要: 【目的】 通过荧光特性评价,探索关键荧光参数对高光效荞麦表征价值的影响,筛选高光效荞麦种质,为其高光效育种提供科学依据和材料支撑。【方法】 采用随机区组大田试验,以154份荞麦品种为试验材料,测定25个叶绿素荧光参数,利用相关性分析、主成分分析和系统聚类-最短距离分析综合评价荞麦荧光特性,并筛选荞麦高光效优良种质资源。【结果】 不同荞麦种质间叶绿素荧光参数均存在极显著差异(P<0.01),其中,PI abs、PI total、ETo/CSm、DIo/RC、REo/CSm、Fv/Fo、TRo/CSm、Fv和Fo/Fm的变异系数较大,CV值均大于25.00%;DF total、DF abs、TRo/RC、DIo/CSm和Fo的变异系数较小,CV值为0.71%~7.97%。除TRo/RC、REo/RC、REo/CSo和PI total外,其余指标的广义遗传力均大于60.00%。主成分分析表明,前4个主成分的累计贡献率达到96.758%,并建立综合评价函数模型A=0.612A1+0.190A2+0.130A3+0.068A4,154份荞麦品种的A值为-2.68~1.20;对A值进行聚类分析,可以将参试品种聚为5类,其中,2个荞麦品种(NQ148和NQ150)被划分为高光效种质资源类型、105个荞麦品种被划分为较高光效种质资源类型、44个荞麦品种被划分为中光效种质资源类型、2个荞麦品种被划分为较低光效种质资源类型、1个荞麦品种被划分为低光效种质资源类型。【结论】 成功筛选出两个具有高光效的荞麦种质NQ148和NQ150,为荞麦种质资源的高光效培育提供了选育材料。

关键词: 荞麦, 叶绿素, 荧光特性, 高光效种质, 综合评价

Abstract: 【Objective】 To explore the effect of key fluorescence parameters on the characterizing value of high light-use efficiency in buckwheat and to screen high light-use efficiency buckwheat germplasm through fluorescence characteristic evaluation,providing a scientific basis and material support for high light-use efficiency breeding.【Methods】 A randomized block field experiment was conducted,using 154 buckwheat varieties as experimental materials. Twenty-five chlorophyll fluorescence parameters were measured. Correlation analysis,principal component analysis (PCA),and systematic clustering-shortest distance analysis were employed to comprehensively evaluate the fluorescence characteristics of buckwheat and identify superior germplasm resources with high light-use efficiency.【Results】 Highly significant differences were observed in chlorophyll fluorescence parameters among different buckwheat varieties(P<0.01). Specifically,parameters PI abs、PI total,ETo/CSm,DIo/RC,REo/CSm,Fv/Fo,TRo/CSm,Fv and Fo/Fm showed larger coefficients of variation(CV) values,all exceeding 25.00%. Parameters DF total,DF abs,TRo/RC,DIo/CSm and Fo showed smaller CV values,ranging from 0.71% to 7.97%. Broad-sense heritability of all other parameters,except TRo/RC,REo/RC,REo/CSo and PI total,was greater than 60.00%. Principal component analysis revealed that the cumulative contribution rate of the top four principal components reached 96.758%,and a comprehensive evaluation function model was established as A=0.612A1+0.190A2+0.130A3+0.068A4,with A values for the 154 buckwheat varieties ranging from -2.68 to 1.20. Clustering analysis of the A values classified the tested varieties into five categories:2 buckwheat varieties(NQ148 and NQ150) were classified as the high light-use efficiency germplasm type,105 varieties were classified as the relatively high light-use efficiency germplasm type,44 varieties were classified as the moderate light-use efficiency germplasm type,2 varieties were classified as the relatively low light-use efficiency germplasm type,and 1 variety was classified as the low light-use efficiency germplasm type.【Conclusion】 Two buckwheat varieties(NQ148 and NQ150) with high light-use efficiency were successfully screened,providing materials for the breeding of high light-use efficiency germplasm resources in buckwheat.

Key words: Buckwheat, Chlorophyll, Fluorescence characteristics, High light-use efficiency germplasm, Comprehensive evaluation

中图分类号: 

  • S517