基于ISSR的8个羊肚菌菌株遗传多样性分析

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Genetic diversity analysis of 8 Morchella esculents strains based on ISSR

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doi:10.12190/j.issn.2096-1197.2024.05.03

Abstract: 【Objective】To clarify the phylogenetic relationship and diversity of Morchella esculents germplasm resources,and to provide reference for the selection and breeding of new varieties adapted to the cultivation in the western region of Inner Mongolia.【Methods】8 Morchella esculents strains（A3,Zhao′erliu,Yangguifei,Jinqiu,DY008,A Lu,001 and Neishilin）were used as experimental materials,ISSR molecular marker technology was used to perform cluster analysis and genetic diversity comparison on 8 strains of Morchella esculents.【Results】Eight Morchella esculents strains were detected by PCR using 100 ISSR primers,12 ISSR primers were selected,and 76 DNA bands were amplified. There were 59 effective bands,and the polymorphism ratio was 75.83%. The gene diversity index （H） was 0.206 6,and Shannon′s information index（I） was 0.330 0. The maximal variation of genetic distance was 1.072 6. When the GS value was about 0.38,the strains were divided into 5 groups.【Conclusion】There was significant genetic variability among the tested Morchella esculents strains.

Key words: Morchella esculents, ISSR, Genetic diversity, Cluster analysis

CLC Number:

S646.7

YU Haibin, BAO Hongchun, LI Xiaolei, REN Yifang, ZONG Hailong, PANG Jie, YU Chuanzong, WANG Yanchun, ZHANG Wenguang. Genetic diversity analysis of 8 Morchella esculents strains based on ISSR[J].Journal of Northern Agriculture, 2024, 52(5): 21-28.

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