Abstract: 【Objective】 To determine the effects of two high-quality subunits,7^OE+8^* and 13+16,on wheat processing quality by utilizing near-isogenic lines under a winter wheat background. 【Methods】 Strong-gluten spring wheat variety Jinqiang No. 1 （containing 7^OE+8^* subunit）and strong-gluten winter wheat variety Gaoyou 2018（containing 13+16 subunit）were crossed. The F₄ and F₅ generation near-isogenic lines materials,in which 7^OE+8^* and 13+16 subunits segregated,were selected and planted in fields and glass greenhouses,respectively. After harvesting,the seeds were ground into wholemeal flour to determine the swelling index of glutenin（SIG）,lactic acid-SDS solvent retention capacity（LA-SDS SRC）,and micro-SDS sedimentation value. 【Results】 Under the genetic backgrounds of subunits 1/5+10 and 1/4+12,the SIG of near-isogenic lines containing the 7^OE+8^* subunit in the F₄ generation was significantly higher than that of the 13+16 subunit（P<0.05）. In the F₅ generation near-isogenic lines,those containing the 7^OE+8^* subunit exhibited higher LA-SDS SRC and micro-SDS sedimentation value. 【Conclusion】 Compared with the 13+16 subunit,the 7^OE+8^* subunit has a greater effect on gluten strength and is a more suitable high-quality subunit for promotion and application in the breeding of strong-gluten winter wheat in China.

Key words: Wheat, 7^OE+8^* subunit, 13+16 subunit, Near-isogenic lines, Processing quality