不同酶谱的复合酶对肉鸡生产性能、血清生化指标及肠道菌群数量的影响

doi:10.12160/j.issn.1672-5190.2021.01.005

摘要/Abstract

摘要： 旨在研究不同酶谱的复合酶对肉鸡生产性能、血清生化指标以及肠道菌群数量的影响。选取480只28日龄的白羽肉鸡,随机分为4组,分别为对照组和试验组Ⅰ、Ⅱ、Ⅲ,每组12个重复,每个重复10只（公、母各半）,对照组饲喂玉米-豆粕型日粮,试验组Ⅰ、Ⅱ、Ⅲ在相同营养水平的日粮中添加不同酶谱的复合酶,添加量均为200 g/t配合料。试验日龄为28~42日龄,试验周期为15 d,分别统计料肉比、测定血清生化指标和肠道菌群数量。结果表明,与对照组相比,试验组Ⅰ、Ⅱ、Ⅲ的料肉比分别降低了1.01%、2.20%和0.83%,差异极显著（P<0.01）;试验组Ⅱ与试验组Ⅰ、Ⅲ相比差异极显著（P<0.01）。与对照组相比,试验组Ⅰ、Ⅱ、Ⅲ的总蛋白、白蛋白的含量分别提高了10.08%~11.41%、12.00%~12.93%,差异极显著（P<0.01）。与对照组相比,试验组Ⅰ、Ⅱ、Ⅲ肠道大肠杆菌的数量分别降低了17.87%、26.43%、21.67%,差异极显著（P<0.01）;试验组Ⅰ与试验组Ⅱ差异极显著（P<0.01）,试验组Ⅲ与试验组Ⅰ、Ⅱ差异显著（P<0.05）。与对照组相比,试验组Ⅰ、Ⅱ、Ⅲ肠道乳酸杆菌的数量分别提高了13.99%、26.10%和24.06%,差异极显著（P<0.01）;试验组Ⅰ与试验组Ⅱ、Ⅲ相比差异极显著（P<0.01）。由此可见,饲料中添加不同酶谱的复合酶对肉鸡生长性能、血清生化指标、肠道菌群数量都有一定的改善作用,同时,不同酶谱的复合酶在改善肉鸡生产性能指标方面幅度有所不同,为生产实践复配高效的复合酶提供依据。

关键词: 复合酶, 生产性能, 生化指标, 菌群数量, 肉鸡

Abstract: The purpose of this study was to assess the effects of compound enzymes with different zymograms on growth performance, serum biochemical indexes and number of intestinal flora of broilers. A total of 480 28-day-old white-feathered broilers were selected and randomly divided into 4 groups with 12 replicates in each group and 10 broilers （half males and half females） in each replicate. The broilers in control group were fed with a corn-soybean meal diet, and those in experimental groups Ⅰ, Ⅱ and Ⅲ fed the same basal diet as control group were supplemented with 200 g/t compound enzymes with different zymograms. The experimental period was 15 days (28 to 42 days of age of the broilers). The ratio of feed to gain, serum biochemical indexes and number of intestinal flora of broilers in different groups were determined and statistically compared. The results showed that compared with the control group, the ratio of feed to gain of experimental groups Ⅰ, Ⅱ and Ⅲ was extremely significantly decreased by 1.01% （P<0.01）, 2.20% （P<0.01） and 0.83% （P<0.01）, respectively; there was extremely significant （P<0.01） difference in ratio of feed to gain among experimental group Ⅱ and experimental groups Ⅰ and Ⅲ. Compared with the control group, the total protein and albumin contents of experimental groups Ⅰ, Ⅱ and Ⅲ were extremely significantly increased by 10.08%-11.41% （P<0.01） and 12.00%-12.93% （P<0.01）, respectively. Compared with the control group, the number of intestinal Escherichia coli in experimental groups Ⅰ, Ⅱ and Ⅲ was extremely significantly decreased by 17.87% （P<0.01）, 26.43% （P<0.01） and 21.67% （P<0.01）, respectively; extremely significant （P<0.01） difference in number of intestinal Escherichia coli between experimental group Ⅰ and experimental group Ⅱ was observed, and significant （P<0.05） difference was also found among experimental group Ⅲ and experimental groups Ⅰ and Ⅱ. Compared with the control group, the number of intestinal Lactobacillus in experimental groups Ⅰ, Ⅱ and Ⅲ was extremely significantly increased by 13.99% （P<0.01）, 26.10% （P<0.01） and 24.06% （P<0.01）, respectively; there was extremely significant （P<0.01） difference in number of intestinal Lactobacillus among experimental group Ⅰ and experimental groups Ⅱ and Ⅲ. In summary, the dietary supplementation of compound enzymes with different zymograms had certain improving effects on growth performance, serum biochemical indexes and number of intestinal flora of broilers. Furthermore, the compound enzymes with different zymograms have varied performance in improving the production performance associated indexes of broilers. The results obtained in this study provide references for collocation of high-efficient compound enzymes in practical production.

Key words: compound enzymes, production performance, biochemical indexes, number of flora, broilers

中图分类号:

S831.5

姜丹, 王丽娟, 丁毅, 黄竹, 唐日益, 解桂香, 徐淼, 张淑枝. 不同酶谱的复合酶对肉鸡生产性能、血清生化指标及肠道菌群数量的影响[J]. 畜牧与饲料科学, 2021, 42(1): 24-28.

JIANG Dan, WANG Li-juan, DING Yi, HUANG Zhu, TANG Ri-yi, XIE Gui-xiang, XU Miao, ZHANG Shu-zhi. Effects of Compound Enzymes with Different Zymograms on Production Performance,Serum Biochemical Indexes and Number of Intestinal Flora of Broilers[J]. Animal Husbandry and Feed Science, 2021, 42(1): 24-28.

参考文献

[1] 邹佳伶. 酶制剂种类与能量水平对肉鸡生产性能和消化生理的影响研究[D].雅安:四川农业大学,2012.
[2] 张芹. 复合酶制剂对肉鸡生产性能及血液生化指标的影响[J].安徽农业科学,2012,40(17):9285-9287.
[3] 杜红方,郭玉光,王敏,等.日粮中添加复合酶对肉鸡生长性能、血液生化指标、肠道形态和屠宰性能的影响[J].中国畜牧杂志,2017(11):96-100.
[4] KACZMAREK S A,ROGIEWICZ A,MOGIELNICKA M,et al.The effect of protease, amylase, and nonstarch polysaccharide-degrading enzyme supplementation on nutrient utilization and growth performance of broiler chickens fed corn-soybean meal-based diets[J].Poultry Science,2014,93(7):1745-1753.
[5] 杜德伟,孙肖明.饲用复合酶制剂对肉鸡的饲养试验[J].吉林工商学院学报,2013,29(2):94-99.
[6] DYMKOV,MALTSEV,张伟,等.饲用复合酶制剂对肉仔鸡生产性能的影响[J].饲料工业,2012,33(10):42-44.
[7] 吕秋凤,董欣,王聪,等.饲粮添加复合酶和制粒对肉仔鸡生产性能、养分代谢率及血液指标的影响[J].饲料工业,2015,36(6):19-24.
[8] 龚俊勇,李芳林,单安山,等.日粮中添加脂肪酶对白羽肉鸡生长性能、血液生化指标、腹脂率的影响[J].饲料工业,2015,36(14):41-45.
[9] BALAMURUGAN R,CHANDRASEKARAN D.Effect of multienzyme supplementation on weight gain, feed intake, feed efficiency and blood glucose in broiler chickens[J].Indian Journal of Science and Technology,2010,3(2):193-195.
[10] 汤海鸥,高秀华,姚斌,等.低能日粮中添加高剂量复合酶对肉鸡血清激素含量和微生物菌群的影响[J].饲料工业,2013,34(18):25-29.
[11] 周小娟,常江,张志帅,等.复合酶对肉鸡生产性能和肠道微生物的影响[J].中国饲料,2013(14):19-22.
[12] 程志斌,苏子峰,张红兵,等.复合非淀粉多糖酶制剂对肉鸡生长性能及肠道酶活性的影响[J].饲料工业,2011,32(16):26-30.
[13] 王冬群,丁雪梅,白世平,等.不同非淀粉多糖复合酶在肉鸡小麦型饲粮中的应用效果[J].动物营养学报,2013,25(10):2371-2385.

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