关键词: 马, 阴道, 格氏乳杆菌, 微生态制剂, 制备工艺

Abstract: [Objective] To determine the process parameters of production technology of a vaginal probiotic preparation using lactic acid bacteria of horse origin. [Method] A Lactobacillus gasseri strain isolated from Mongolian horse vagina, was used as a starter to product the probiotic preparation. Fermentation technology and response surface method were employed to optimize the strain′s fermentation medium and process parameters as well as the probiotic preparation′s formulation and process parameters. [Result] Lactose and soybean peptone had significant impacts on growth performance of the bacterial powder. When the initial pH value of the fermentation broth was set to 6.6, the temperature was set to 40 ℃, and the inoculation amount was set to 6%, the fermentation performance of the bacterial powder was largely influenced. Using pharmaceutical preparation technology and response surface method, the probiotic preparation′s formulation and process parameters were then adjusted. When the mixing ratio of bacterial powder to starch, micro-powder silica gel, and micro-crystalline cellulose was 167.05∶1.61∶1 and the rotating speed of multi-functional medicine machine was 20 r/min at 60 ° angle, the number of live bacteria in the obtained probiotic preparation was higher. [Conclusion] The critical process parameters for production of vaginal probiotic preparation with horse origined Lactobacillus gasseri were determined, providing a foundation for further explorations on the use of probiotic preparations made by live bacteria to prevent and treat horse vaginal infection.

Key words: horse, vagina, Lactobacillus gasseri, probiotic preparation, production technology