关键词: 里岔黑猪, 益生菌, 酶制剂, 肠道菌群, 菌酶协同

Abstract:

[Objective] This study was conducted to investigate the synergistic effects of feeding bacteria and enzymes on growth performance, serum immune indexes and intestinal microflora of weaned piglets of Licha black pig. [Method] A total of 80 healthy 28-day-old weaned piglets of Licha black pig with body weights of （6.72±0.12） kg were randomly divided into the following four groups: a control group fed with basal diet, a probiotic group fed with basal diet supplemented with 1‰ of probiotic, an enzyme preparation group fed with basal diet supplemented with 1‰ enzyme preparation, and a bacteria and enzymes synergistic group fed with basal diet supplemented with 1‰ probiotic and 1‰ enzyme preparation. The experiment lasted for 35 d, including a 7 d pre-feeding period and a 28 d formal feeding period. The average daily gain, average daily feed intake, ratio of feed to weight gain and diarrhea index were calculated, and the serum levels of IgM and IgG were determined. The composition and relative abundance of intestinal micoflora were examined by 16S rDNA high-throughput sequencing. [Result] Compared with the control group, the average daily feed intake of the probiotic group, enzyme preparation group, and bacteria and enzymes synergistic group increased significantly （P<0.05）; the enzyme preparation group and bacteria and enzymes synergistic group had significantly （P<0.05） increased average daily gain and significantly （P<0.05） decreased ratio of feed to weight gain; the diarrhea index of probiotic group and bacteria and enzymes synergistic group extremely significantly （P<0.01） reduced. The probiotic group, enzyme preparation group, and bacteria and enzymes synergistic group had significantly （P<0.05） higher serum levels of IgM and IgG than control group. Significantly （P<0.05） higher Chao1 index of intestinal microflora was observed in probiotic group, enzyme preparation group, and bacteria and enzymes synergistic group compared with control group. At the phylum level, the relative abundance of Bacteroides in probiotic group as well as bacteria and enzymes synergistic group was significantly （P<0.05） higher than that in control group. [Conclusion] The bacteria and enzymes synergistic feeding mode increased the average daily feed intake and average daily gain, reduced the diarrhea index, enhanced the body immunity, and improved the intestinal microbial community structure of weaned piglets of Licha black pig.

Key words: Licha black pig, probiotic, enzyme preparation, intestinal microflora, bacteria and enzymes synergism