内蒙古呼伦贝尔地区本土牛牛肉黄脂现象的相关基因和代谢组学研究

doi:10.12160/j.issn.1672-5190.2023.06.001

畜牧与饲料科学 ›› 2023, Vol. 44 ›› Issue (6): 1-12.doi: 10.12160/j.issn.1672-5190.2023.06.001

• 科技创新支撑畜牧业高质量发展专栏——新理论新技术在畜牧业中的应用专题 • 下一篇

内蒙古呼伦贝尔地区本土牛牛肉黄脂现象的相关基因和代谢组学研究

王潇¹, 李慧¹, 苏少锋¹, 赵濛¹, 田菁¹, 赵鸿雁², 左兰明³, 梁智杰⁴, 王金环⁵, 田如刚¹

1.内蒙古自治区农牧业科学院,内蒙古呼和浩特 010031;
2.鄂托克旗农牧技术推广中心,内蒙古鄂托克旗 016199;
3.赤峰市敖汉旗农牧局,内蒙古敖汉旗 024300;
4.赤峰市农牧技术推广中心,内蒙古赤峰 024031;
5.赤峰市农牧局,内蒙古赤峰 024050

收稿日期:2023-03-28 出版日期:2023-11-30 发布日期:2024-01-11
通讯作者: 田如刚（1978—）,男,研究员,博士,主要研究方向为肉牛遗传育种。
作者简介:王潇（1987—）,男,助理研究员,硕士,主要研究方向为肉牛遗传育种。
基金资助:
内蒙古农牧业青年创新基金项目（2021QNJJM07）; 国家自然科学基金项目（32060731）

Genetic Variant Analysis and Metabolomics Reveal the Possible Molecular Mechanisms Underlying Yellow Beef Fat of Local Cattle in Hulun Buir City of Inner Mongolia

WANG Xiao¹, LI Hui¹, SU Shaofeng¹, ZHAO Meng¹, TIAN Jing¹, ZHAO Hongyan², ZUO Lanming³, LIANG Zhijie⁴, WANG Jinhuan⁵, TIAN Rugang¹

1. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;
2. Agricultural and Animal Husbandry Technology Extension Center of Otog Banner,Otog Banner 016199,China;
3. Agricultural and Animal Husbandry Bureau of Aohan Banner of Chifeng City,Aohan Banner 024300,China;
4. Agricultural and Animal Husbandry Technology Extension Center of Chifeng City,Chifeng 024031,China;
5. Agricultural and Animal Husbandry Bureau of Chifeng City,Chifeng 024050,China

Received:2023-03-28 Online:2023-11-30 Published:2024-01-11

摘要/Abstract

摘要： [目的]初步探索内蒙古呼伦贝尔地区本土牛牛肉黄脂现象可能的调控机制。[方法]从呼伦贝尔市选取72头本土牛,每头牛均采集皮下脂肪及肝脏组织样本。采用5分制主观评分方法,对皮下脂肪样本进行颜色评分。以肝脏组织为试验材料,利用五引物扩增受阻突变体系（penta-primer amplification refractory mutation system,PARMS）分析参与β-胡萝卜素代谢过程的相关通路中关键基因BCO2 SNPW80X、BCMO1 SNP4、RDHE2 SNP2、ALDH8A1 SNP16和PPARCG1A SNP12的基因型及基因频率分布情况。运用液相色谱-质谱联用（LC-MS）技术对脂肪样本进行非靶向代谢组学检测,挖掘不同颜色脂肪样本的代谢差异物,并将差异代谢物进行代谢通路富集分析。[结果]72份皮下脂肪组织以白色为主,黄色脂肪（评分3分及以上）比例占到30.55%,BCO2、BCMO1、RDHE2和PPARCG1A等基因在不同样本中存在SNP差异,但这些差异与脂肪颜色无显著（P>0.05）相关性。黄色脂肪样本与白色脂肪样本相比,共鉴定到235个差异代谢物,这些差异代谢物可富集到不饱和脂肪酸生物合成、初级胆汁酸生物合成和胆汁分泌等信号通路。[结论]呼伦贝尔地区本土牛牛肉黄色脂肪组织样本与白色脂肪组织样本相比,有多个代谢物存在差异,涉及的信号通路与脂肪形成、体内β-胡萝卜素转运和β-胡萝卜素跨膜运输之间存在密切关系,且与BCO2、BCMO1、PPARCG1A等β-胡萝卜素代谢相关基因直接或间接相关。后续可对相关分子调控机制开展进一步研究,为含β-胡萝卜素饲料的制备和定向调节肉牛脂肪颜色提供理论基础。

关键词: 呼伦贝尔, 肉牛, 黄脂现象, SNP差异, 代谢组学, β-胡萝卜素

Abstract: [Objective] This study was conducted to preliminarily explore the possible regulative mechanisms underlying yellow beef fat of local cattle in Hulun Buir City of Inner Mongolia. [Method] A total of 72 local cattle were selected from Hulun Buir City, and their subcutaneous fat and liver tissues were sampled. The subcutaneous fat samples were subjected to color scoring with a 5-point subjective scoring scheme. Using liver tissues as experimental materials, the penta-primer amplification refractory mutation system （PARMS） was employed to assess the genotype and gene frequency distribution of BCO2 SNPW80X, BCMO1 SNP4, RDHE2 SNP2, ALDH8A1 SNP16 and PPARCG1A SNP12, which were considered as the crucial genes in the signaling pathways involved in the metabolic process of β-carotene. The non-targeted metabolomics analysis of the fat samples were performed by liquid chromatography-mass spectrometry （LC-MS） technology to identify the differential metabolites in different colored fat samples, and the differential metabolites were subsequently subjected to metabolic pathway enrichment analysis. [Result] The 72 subcutaneous fat tissue samples were predominantly white colored, with yellow colored ones （scoring 3 or above） accounting for 30.55%. The SNPs in BCO2, BCMO1, RDHE2 and PPARCG1A genes were observed in different samples, while these SNPs had no significant （P>0.05） correlation with fat color. Compared with the white colored fat samples, a total of 235 differential metabolites were identified in the yellow colored fat samples, which were enriched in the signaling pathways such as unsaturated fatty acid biosynthesis, primary bile acid biosynthesis, and bile secretion. [Conclusion] Comparing the yellow and white colored fat samples collected from the local cattle in Hulun Buir City, there were multiple differential metabolites involving in the signaling pathways closely related to fat formation, in vivo β-carotene transport and trans-membrane transport of β-carotene. In addition, they also directly or indirectly correlated with the β-carotene metabolism associated genes such as BCO2, BCMO1 and PPARCG1A. Further research may focus on the relevant molecular regulatory mechanisms, so as to provide a theoretical basis for the preparation of β-carotene containing feed and the targeted regulation of beef fat color.

Key words: Hulun Buir City, beef cattle, yellow colored fat, SNP, metabolomics, β-carotene

中图分类号:

S823.922

王潇, 李慧, 苏少锋, 赵濛, 田菁, 赵鸿雁, 左兰明, 梁智杰, 王金环, 田如刚. 内蒙古呼伦贝尔地区本土牛牛肉黄脂现象的相关基因和代谢组学研究[J]. 畜牧与饲料科学, 2023, 44(6): 1-12.

WANG Xiao, LI Hui, SU Shaofeng, ZHAO Meng, TIAN Jing, ZHAO Hongyan, ZUO Lanming, LIANG Zhijie, WANG Jinhuan, TIAN Rugang. Genetic Variant Analysis and Metabolomics Reveal the Possible Molecular Mechanisms Underlying Yellow Beef Fat of Local Cattle in Hulun Buir City of Inner Mongolia[J]. Animal Husbandry and Feed Science, 2023, 44(6): 1-12.

参考文献

[1] 木其尔. 呼伦贝尔蒙古牛黄色脂肪成因物质研究[D].呼和浩特:内蒙古农业大学,2016.
[2] 刘晓牧,吴乃科,宋恩亮,等.牛肉黄脂研究进展[J].中国牛业科学,2006,32(2):31-33.
[3] SIMONNE A H,GREEN N R,BRANSBY I D.Consumer acceptability and β-carotene content of beef as related to cattle finishing diets[J].Journal of Food Science,1996,61(6):1254-1257.
[4] JIN Q,CHENG H J,WAN F C,et al.Effects of feeding β-carotene on levels of β-carotene and vitamin A in blood and tissues of beef cattle and the effects on beef quality[J].Meat Science,2015,110:293-301.
[5] 梅步俊,李亚珍,吉日嘎拉,等.反刍动物黄脂研究进展[J].草食家畜,2013(4):1-5.
[6] RHRLE F T,MOLONEY A P,OSORIO M T,et al.Carotenoid,colour and reflectance measurements in bovine adipose tissue to discriminate between beef from different feeding systems[J].Meat Science,2011,88(3):347-353.
[7] SHEMEIS A R,LIBORIUSSEN T,ANDERSEN B B,et al.Changes in carcass and meat quality traits of Danish friesian cull cows with the increase of their age and body condition[J].Meat Science,1994,37(2):161-167.
[8] WALSH K,KIELY P O,MOLONEY A P,et al.Intake,digestibility,rumen fermentation and performance of beef cattle fed diets based on whole-crop wheat or barley harvested at two cutting heights relative to maize silage or ad libitum concentrates[J].Animal Feed Science and Technology,2008,144(3/4):257-278.
[9] WANG Y M,CONNOR S L,WANG W,et al.The selective retention of lutein,meso-zeaxanthin and zeaxanthin in the retina of chicks fed a xanthophyll-free diet[J].Experimental Eye Research,2007,84(3):591-598.
[10] JOHANNES V L,KLAUS V.Vitamin A formation in animals:Molecular identification and functional characterization of carotene cleaving enzymes[J].Journal of Nutrition,2004,134(1):251S-256S.
[11] LIETZ G,LANGE J,RIMBACH G.Molecular and dietary regulation of β,β-carotene 15,15′-monooxygenase 1(BCMO1)[J].Archives of Biochemistry and Biophysics,2010,502(1):8-16.
[12] KIEFER C,HESSEL S,LAMPERT J M,et al.Identification and characterization of a mammalian enzyme catalyzing the asymmetric oxidative cleavage of provitamin A[J].Journal of Biological Chemistry,2001,276(17):14110-14116.
[13] TIAN R,PITCHFORD W S,MORRIS C A,et al.Genetic variation in the β,β-carotene-9′,10′-dioxygenase gene and association with fat colour in bovine adipose tissue and milk[J].Animal Genetics,2010,41(3):253-259.
[14] TIAN R,CULLEN N G,MORRIS C A,et al.Major effect of retinal short-chain dehydrogenase reductase(RDHE2)on bovine fat colour[J].Mammalian Genome,2012,23(5/6):378-386.
[15] 魏巧丽,成海建,游伟,等.β-胡萝卜素-15,15′-加氧酶在动物营养及遗传领域的研究进展[J].动物营养学报,2013,25(12):2838-2843.
[16] 任晓莹,王树林,曹得霞.日粮中添加β-胡萝卜素对牦牛皮下脂肪颜色及组织中维生素A含量的影响[J].中国畜牧兽医,2020,47(9):2833-2840.
[17] KNIGHT T W,DEATH A F.Effect of dose and frequency of vitamin A supplements,and carryover effects on plasma carotenoid concentration in steers[J].New Zealand Journal of Agricultural Research,1999,42(4):385-391.
[18] 魏巧丽. β-胡萝卜素对肉牛脂肪合成的影响研究[D].泰安:山东农业大学,2014.
[19] JOHANNES V L.Provitamin A metabolism and functions in mammalian biology[J].The American Journal of Clinical Nutrition,2012,96(5):1234S-1244S.
[20] BERTOLINI F,CHINCHILLA-VARGAS J,KHADSE J R,et al.Marker discovery and associations with beta-carotene content in Indian dairy cattle and buffalo breeds[J].Journal of Dairy Science,2019,102(11):10039-10055.
[21] 徐磊,贾玉堂,赵拴平,等.西门塔尔牛BCO2基因多态性及与脂肪颜色性状的相关性研究[J].中国牛业科学,2015,41(3):22-26.
[22] LIETZ G,OXLEY A,BOESCH‐SAADATMANDI C,et al. Importance of β,β‐carotene 15,15′‐monooxygenase 1(BCMO1)and β,β‐carotene 9′,10′‐dioxygenase 2(BCDO2)in nutrition and health[J].Molecular Nutrition and Food Research,2012,56(2):241-250.
[23] 樊月圆,昝林森,王洪宝,等.牛过氧化物酶体增殖激活受体α(PPARα)基因第5外显子多态性与胴体、肉质性状的相关性[J].农业生物技术学报,2010,18(4):713-718.
[24] AL-WADEI H A N,SCHULLER H M. Non-genomic inhibitory signaling of β-carotene in squamous cell carcinoma of the lungs[J].International Journal of Oncology,2009,34(4):1093-1098.
[25] KNIGHT T W,DEATH A F.Is beef with yellow fat potentially healthier for you than beef with white fat[J].Proceedings of the New Zealand Society of Animal Production,1997,57:134-136.
[26] BACHMANN H,DESBARATS A,PATTISON P,et al.Feedback regulation of β,β-carotene 15,15′-monooxygenase by retinoic acid in rats and chickens[J].Journal of Nutrition,2003,132(12):3616-3622.
[27] 韩瑞丽. β-胡萝卜素和VE对富含ω-3多不饱和脂肪酸鸡肉的保护作用和肉质的影响[D].泰安:山东农业大学,2003.
[28] SHAMA S,LIU W Q.Omega-3 fatty acids and gut microbiota:A reciprocal interaction in nonalcoholic fatty liver disease[J].Digestive Diseases and Sciences,2020,65(3):906-910.
[29] HESSEL S,EICHINGER A,ISKEN A,et al.CMO1 deficiency abolishes vitamin A production from β-carotene and alters lipid metabolism in mice[J].Journal of Biological Chemistry,2007,282(46):33553-33561.
[30] SAEED A,DULLAART R P F,SCHREUDER T C M A,et al. Disturbed vitamin A metabolism in non-alcoholic fatty liver disease(NAFLD)[J].Nutrients,2017,10(1):29.
[31] SEO J S,LEE K S,JANG J H,et al.The effect of dietary supplementation of β-carotene on lipid metabolism in streptozotocin-induced diabetic rats[J].Nutrition Research,2004,24(12):1011-1021.
[32] HOEKE M O.The interrelationship between bile acid and vitamin A homeostasis[D]. Groningen:University of Groningen,2017.
[33] BOHLOULI M,HALLI K,YIN T,et al.Genome-wide associations for heat stress response suggest potential candidate genes underlying milk fatty acid composition in dairy cattle[J].Journal of Dairy Science,2022,105(4):3323-3340.
[34] KASSAM A,MIAO B,YOUNG P R,et al.Retinoid X receptor(RXR)agonist-induced antagonism of farnesoid X receptor(FXR)activity due to absence of coactivator recruitment and decreased DNA binding[J].Journal of Biological Chemistry,2003,278(12):10028-10032.
[35] JEMIOA-RZEMINSKA M,PASENKIEWICZ-GIERULA M,STRZAKA K.The behaviour of beta-carotene in the phosphatidylcholine bilayer as revealed by a molecular simulation study[J].Chemistry and Physics of Lipids,2005,135(1):27-37.
[36] DOPIERA K,SKRZYPIEC M.Morphology,compressibility and viscoelasticity of the mixed lipid monolayers in the presence of β-carotene[J].Chemistry and Physics of Lipids,2018,213:88-95.
[37] 武陶,张柏林,毕昌昊.细胞膜合成途径模块化调控与形态改造提高大肠杆菌β-胡萝卜素的积累与产量[J].生物工程学报,2018,34(5):79-87.

内蒙古呼伦贝尔地区本土牛牛肉黄脂现象的相关基因和代谢组学研究

Genetic Variant Analysis and Metabolomics Reveal the Possible Molecular Mechanisms Underlying Yellow Beef Fat of Local Cattle in Hulun Buir City of Inner Mongolia

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	张俊丽, 侯鹏霞, 梁小军. 南瓜籽壳对不同生长阶段西杂肉牛生长性能、血清生化和免疫指标及激素水平的影响[J]. 畜牧与饲料科学, 2024, 45(1): 20-24.
[2]	陈炯阳, 程艳, 伍志武, 王标, 杨燕燕, 杨青, 贺志雄, 谭支良. 澳洲白羊与呼伦贝尔羊杂交对母羊繁殖性能及妊娠期生殖激素分泌的影响[J]. 畜牧与饲料科学, 2024, 45(1): 42-47.
[3]	张俊丽, 摆世林, 侯鹏霞, 梁小军. 藜麦秸秆微贮替代全株青贮玉米对西杂育肥牛生产性能、营养物质表观消化率及血清指标的影响[J]. 畜牧与饲料科学, 2023, 44(4): 41-46.
[4]	张利妹,郭军,叶乐,刘雨佳,刘晓齐,郭灵芝. 内蒙古呼伦贝尔草原短尾羊内脏Se含量检测评价[J]. 畜牧与饲料科学, 2023, 44(4): 101-108.
[5]	马贵, 安彦昊, 马莉花, 沙萍, 马燕芬. 代谢组学技术在筛选奶牛脂肪肝生物标志物中的应用研究进展[J]. 畜牧与饲料科学, 2023, 44(1): 38-43.
[6]	戴雪, 崔金磊, 吴彤, 王立东, 于树海, 李旭光, 李婷, 张志宏. 内蒙古通辽市肉牛种业发展进程、存在问题及对策[J]. 畜牧与饲料科学, 2022, 43(1): 88-92.
[7]	王尚扬, 栗文钰, 次旦曲尼, 仁增旺姆, 王彦芦, 冯景利, 何昌伟, 莫放. 肉牛不同生长阶段日粮营养物质表观消化率的比较[J]. 畜牧与饲料科学, 2021, 42(6): 9-12.
[8]	木其儿, 杨苏日娜, 宝希吉日, 吴金虎, 根锁. 散养肉牛饲养成本收益及影响因素分析——以我国五大散养肉牛主产省（区）为例[J]. 畜牧与饲料科学, 2021, 42(6): 92-101.
[9]	杨苏日娜, 木其儿, 吴金虎, 杜春玲, 根锁. 肉牛产业领域研究热点与前沿 ——基于CNKI数据库的文献计量[J]. 畜牧与饲料科学, 2021, 42(5): 98-107.
[10]	赵濛, 苏日娜, 王潇, 李慧, 杨鼎, 何云梅, 王峰, 栾庆江, 赛音, 郭文华, 田春英, 田如刚. 内蒙古鄂尔多斯市鄂托克旗肉牛产业现状及发展对策[J]. 畜牧与饲料科学, 2021, 42(2): 80-84.
[11]	郭晨阳, 王丽芳, 宋洁. 2019年呼伦贝尔地区奶牛产奶量及质量指标不同月份变化分析[J]. 畜牧与饲料科学, 2021, 42(1): 60-62.
[12]	郑海英, 李九月, 薛树媛, 王晓鹏, 贾伟星. 微生物制剂处理秸秆日粮对肉牛生产性能和养殖效益的影响[J]. 畜牧与饲料科学, 2020, 41(5): 37-40.
[13]	肖燕子, 黄学文, 乌仁其其格, 孙林, 丁霞, 侯美玲, 张培青. 不同温度处理对呼伦贝尔天然草地3种野生豆科牧草种子萌发的影响[J]. 畜牧与饲料科学, 2020, 41(4): 40-44.
[14]	韦人月, 郑家三. 代谢组学技术在奶牛生产性疾病研究中的应用[J]. 畜牧与饲料科学, 2020, 41(1): 18-22.
[15]	赵俊利, 侯智惠, 侯安宏, 高晓霞, 王秀文. 内蒙古呼伦贝尔市肉牛产业现状及发展对策分析[J]. 畜牧与饲料科学, 2019, 40(9): 65-69.